Development of a novel cross-axis countercurrent chromatographic instrument with six separation columns: Design, dynamics, optimization, prototyping, and experiment.

This paper presents the development of a novel cross-axis countercurrent chromatographic instrument (CCCI) with six separation columns, including design, dynamics, optimization, prototype construction, and experimental validation. The conceptual design and the structural design of the cross-axis CCCI are performed while considering the requirements for the separation operation and design. The dynamic analysis is carried out in order to guarantee the local balance and the global balance for the CCCI.