Living Cell-Mediated Self-Assembly: From Monomer Design and Morphology Regulation to Biomedical Applications.

The self-assembly of molecules into highly ordered architectures is a ubiquitous and natural process, wherein molecules spontaneously organize into large structures to perform diverse functions. Drawing inspiration from the formation of natural nanostructures, cell-mediated self-assembly has been developed to create functional assemblies both inside and outside living cells. These techniques have been employed to regulate the cellular world by leveraging the dynamic intracellular and extracellular microenvironment.

Inhibition of Abdominal Aortic Aneurysm Progression Through the CXCL12/CXCR4 Axis via MiR206-3p Sponge.

Notably, the C-X-C Motif Chemokine Ligand 12/C-X-C Chemokine Receptor Type 4 (CXCL12/CXCR4) signalling pathway's activation is markedly increased in a mouse model of abdominal aortic aneurysms (AAA). Nonetheless, the precise contribution of this pathway to AAA development remains to be elucidated. The AAA mouse model was induced by local incubation with elastase and oral administration of β-aminopropionitrile.

Targeting Synthesis of Diatomic Catalysts by Selective Etching and Sequential Adsorption of Metal Atom.

Diatomic catalysts featuring a tunable structure and synergetic effects hold great promise for various reactions. However, their precise construction with specific configurations and diverse metal combinations is still challenging. Here, a selective etching and metal ion adsorption strategy is proposed to accurately assign a second metal atom (M) geminal to the single atom site (M-N) for constructing diatomic sites (e.

Partially observable Markov models inferred using statistical tests reveal context-dependent syllable transitions in Bengalese finch songs.

Generative models have diverse applications, including language processing and birdsong analysis. In this study, we demonstrate how a statistical test, designed to prevent overgeneralization in sequence generation, can be used to infer minimal models for the syllable sequences in Bengalese finch songs. We focus on the partially observable Markov model (POMM), which consists of states and the probabilistic transitions between them.

Effects of stir-frying on chemical profile, sensory quality and antioxidant activity of Chrysanthemi Flos: A metabolomics and sensory study.

Chrysanthemi Flos has been consumed as floral tea for centuries, but the effects of stir-frying on its chemical profile, sensory characteristics, and bioactivity remain unclear. This study used untargeted metabolomics, sensory assessment (E-eye, E-nose, E-tongue), and antioxidant activity evaluation to investigate compositional changes and their effects. In the metabolomics analysis, a total of 101 non-volatile and 306 volatile differential metabolites were identified.

Characterization, adsorption kinetic and in vitro release behavior of curcumin loaded with porous mannitol and porous lactose: Template agent method vs. Pore-forming agent method.

Polyvinylpyrrolidone K30 was used as the templating agent, and ammonium bicarbonate was used as the pore-forming agent to make porous mannitol and porous lactose by the template and pore-forming agent method, respectively. Compared with the template method, the porous particles prepared by the pore-forming agent method have larger pore diameter (320.276 nm and 250.

Unlocking dual-mode enzyme activities on bacterial surface: Directional recognition and swift capture of Alicyclobacillus acidoterrestris from fruit juices.

The acidophilic and heat-resistant characteristics of Alicyclobacillus acidoterrestris (A. acidoterrestris) pose significant challenges to fruit juice production. Traditional thermal removal methods are often ineffective against this resilient bacterium.

Consumer evaluations of commercially available plant-based cheddar cheese alternatives reveal inferior taste, nutrition and emotional appeal.

Many people in the Western world wish to reduce dietary reliance on animal-based and animal-derived foods. Plant-based (PB) meat and dairy alternatives can aid in this transition, but in the dairy category, only the milk market is well developed. Attention in the present research is, therefore, directed to PB cheese alternatives (PBCA), which were studied relative to dairy cheeses in a consumer taste test (central location setting) with 157 New Zealand (NZ) consumers conducted in 2023.

Adhesive conductive wood-based hydrogel with high tensile strength as a flexible sensor.

Conductive hydrogels have promising applications for flexible strain sensors. However, most hydrogels have poor tensile strength and are susceptible to damage, significantly impeding their potential for further application. Wood has been used to reinforce hydrogels, significantly enhancing their strength and dimensional stability.

In situ growth of defective ZIF-8 on TEMPO-oxidized cellulose nanofibrils for rapid response release of curcumin in food preservation.

Uncontrolled release of active agents in active packaging reduces antimicrobial efficacy, hindering the effective protection of perishable products from microbial infection. Herein, a novel defective engineering was proposed to design defective and hollow ZIF-8 structures grown on TEMPO oxidized cellulose nanofibrils (TOCNFs) and use them as fast-reacting nanocarriers for loading and controlled release curcumin (Cur) in sodium alginate (SA) active packaging systems (CZT-Cur-SA). By employing stable chelation between tannic acid (TA) and ZIF-8 zinc ions, the connections between zinc ions and imidazole ligands were severed to form a loose and hollow structure, which facilitates the rapid reaction and release of active ingredients triggered by pH changes in the microenvironment.

Biocompatible dually reinforced gellan gum hydrogels with selective antibacterial activity.

The poor mechanics and functionality of natural-polymer hydrogels from gellan gum (GG) prohibit their practical application, despite the intrinsic thermo-reversible gelation nature, structural and quality consistency, biocompatibility, biodegradability and sustainability of microbial fermentation-produced GG. Herein, a dual-reinforcing strategy, i.e.

Preparation of agar polysaccharides and biological activities and relationships of agar-derived oligosaccharides and monosaccharides: A review.

Agar is one of the three major colloidal linear polysaccharides obtained from marine seaweeds, specifically red macroalgae (Rhodophyta). It has garnered significant attention owing to its diverse industrial applications, potential for bioethanol production, and the physiological activities of its derived saccharides. This review delves into the preparation and degradation processes of agar, focusing on both physical and chemical pretreatments, as well as subsequent hydrolysis through acid and enzymatic methods.

Mechanism of Traditional Chinese Medicine extract in the treatment of diabetic erectile dysfunction.

Ethnopharmacological Relevance: Diabetic erectile dysfunction (DED) is a prevalent but often overlooked microvascular complication of type 2 diabetes mellitus (T2DM), with strong associations to cardiovascular disease. The pathophysiology of erectile dysfunction (ED) in T2DM patients is more intricate than in non-diabetic individuals, likely involving multiple pathogenic mechanisms such as endothelial dysfunction, vascular alterations, neuropathy, and oxidative stress. Traditional Chinese Medicine (TCM) has long been utilized in the management of DED, drawing on an extensive body of clinical experience.

Chromosome-level echidna genome illuminates evolution of multiple sex chromosome system in monotremes.

Background: A thorough analysis of genome evolution is fundamental for biodiversity understanding. The iconic monotremes (platypus and echidna) feature extraordinary biology. However, they also exhibit rearrangements in several chromosomes, especially in the sex chromosome chain.

Effects of environment regulating T4SS on virulence and adaptability of Streptococcus suis.

Streptococcus suis (S. suis) represents a significant bacterial pathogen, with its zoonotic transmission from infected or deceased pigs to humans posing a serious threat to public health. The type IV secretion system (T4SS), a critical virulence factor of S.

An electrochemical immunosensor for carbohydrate antigen CA125 based on electroactive COFs as a signal probe.

Levels of CA125 are strongly associated with cervical, pancreatic, bowel and breast cancer. However, the common CA125 detection method has the disadvantages of poor repeatability, high cost, easy to be disturbed and poor stability. In this work, a COF based electrochemical immunosensor was developed for the rapid, sensitive and stable detection of CA125.

Construction of naphthalimide-based fluorescent probe for sequential detection of Al and NOR and its application in tea and honey.

Metal aluminum ions and the antibiotic NOR have both benefited humanity and due to improper use, posed significant threats to the environment and human health. Although the correct and rational use of these substances is crucial, developing real-time, rapid, and highly sensitive detection of trace aluminum ions and NOR in the environment and organisms is more important for early diagnosis of potential related diseases. After several decades of development, fluorescence analysis has become a mainstream research method due to its ability to meet these requirements.

REBOA in patients with high-grade liver injury may be associated with worse outcomes.

Background: Although controversial, resuscitative endovascular balloon occlusion of the aorta (REBOA) has been used to manage liver injuries. This matched cohort study evaluated outcomes in severe liver injuries treated with REBOA or without REBOA.

Methods: Trauma Quality Improvement Program database study.

Engineered cell membrane vesicles loaded with lysosomophilic drug for acute myeloid leukemia therapy via organ-cell-organelle cascade-targeting.

Acute myeloid leukemia (AML) presents significant treatment challenges due to the severe toxicities and limited efficacy of conventional therapies, highlighting the urgency for innovative approaches. Organelle-targeting therapies offer a promising avenue to enhance therapeutic outcomes while minimizing adverse effects. Herein, inspired that primary AML cells are enriched with lysosomes and sensitive to lysosomophilic drugs (e.

Development and validation of an UPLC-MS/MS method with polarity switching for simultaneous determination of 14 antiepileptic drugs and 2 metabolites in human serum.

Currently, treatment with antiepileptic drugs (AEDs) is still the first choice for epileptic patients, while monitoring their blood concentrations is undoubtedly beneficial for minimizing their adverse side effects and optimizing their therapeutic effects. In this study, an ultra-high performance liquid chromatography coupled with tandem mass spectrometry with polarity switching was developed and validated for simultaneous determination of 14 AEDs and 2 active metabolites in human serum. Olanzapine was selected as the internal standard.

Anti-Inflammatory Withanolides From Physalis angulata Var. villosa Bonati.

Two new withanolide derivatives (1 and 2) and 12 known withanolide analogs (3-14) were identified from the aerial part of Physalis angulata var. villosa Bonati. Their structures were determined by various spectral data, quantum chemical calculations, and sugar derivatization methods.

A Cohort Clinical Study on the Efficacy of Topical Salicylic Acid/Piroctone Olamine Dandruff Pre-Gel and Cleanser in Improving Symptoms of Moderate to Severe Seborrheic Dermatitis of the Scalp.

Background: Scalp seborrheic dermatitis (SD) is a chronic, recurrent inflammatory skin condition associated with scalp sebum secretion and dysbiosis.

Aims: The aim of this study is to evaluate the efficacy and safety of a topical salicylic acid/piroctone olamine/zinc salt of L-pyrrolidone carboxylate (Zinc PCA) scalp pre-application gel in combination with a salicylic acid/piroctone olamine/antimicrobial peptide cleansing lotion for the treatment of moderate to severe scalp SD.

Patients/methods: In this prospective cohort study, 20 patients with moderate to severe scalp SD were treated with a combination of the scalp pre-application gel and cleansing lotion for 4 weeks (one tube of the pre-application gel per week and the cleansing lotion used every 1-3 days depending on the frequency of hair washing).

Virus Filtration Development for Adeno-Associated Virus-Based Gene Therapy Products.

Adeno-associated virus (AAV) vectors have become a leading platform for gene delivery. A major portion of gene therapy currently in clinical trials are AAV-based for a wide range of diseases. A commonly used method for AAV production is by mammalian or insect cell culture, with or without added viruses to introduce needed genetic elements for AAV production.