A fast and efficient method for isolating Schwann cells from sciatic nerves of neonatal mice.

Background: Schwann cells (SCs) isolation is one of the basic techniques for study of peripheral nervous system and peripheral neuropathy. A combined and effective method of isolating SCs from sciatic nerves of newborn mice with high yield and purity is still lacking.

New Methods: Sciatic nerves from neonatal mice aged 3-5 days serve as the source of SCs.