Tissue-engineered blood vessel graft produced by self-derived cells and allogenic acellular matrix: a functional performance and histologic study.

Introduction: Current prosthetic alternatives to autologous vascular grafts remain poor in terms of patency. Growing biologic blood vessels has been proposed as an alternative. In this work, the authors demonstrate a method for producing a tissue-engineered vascular graft (TEVG) with self-derived endothelial cells, smooth muscle cells, and allogenic acellular matrix in vitro.

Decellularized aorta of fetal pigs as a potential scaffold for small diameter tissue engineered vascular graft.

Background: For cardiovascular tissue engineering, acellularized biomaterials from pig have been widely investigated. Our purpose was to study mechanical properties and biocompatibility of decellularized aorta of fetal pigs (DAFP) to determine its potential as scaffold for small diameter tissue engineered vascular graft.

Methods: Descending aorta of fetal pigs was removed cells using trypsin, ribonuclease and desoxyribonuclease.

[An experimental study of small-caliber tissue engineering vessels with acellular matrix].

Objective: To observe the mechanical properties of the prefabricated connective tissue tube as blood vessel substitute and its changes after implantation at the femoral artery.

Methods: The acellular matrix tube of 8-12 cm in length with a silicone rod inside it was implanted into dog peritoneal cavity. 3 weeks later, a new formed tube around the silicone rod was transferred to the femoral artery as blood vessel substitute.

Establishing a new orthotopic composite hemiface/calvaria transplantation model in rabbits.

Background: Composite tissue allograft transplantation provides a better reconstructive option for patients who suffer from extensive craniomaxillofacial deformities. However, there is a lack of sufficient experimental data including anatomical and immunologic aspects in larger animals. The purpose of this study was to develop a new orthotopic composite hemiface/calvaria transplantation model in rabbits with which to estimate the feasibility of composite tissue allografts.

[Biocompatibility of decellularized canine carotid artery allograft cross-linked by carbodiimide].

Unlabelled: OBJECTIVE Crosslink decellularized canine carotid artery allograft by EDC [1-3-(dimethylamino)propyl-3-ethylcarbodiimide methiodide] and evaluate the biocompatibility of it.

Methods: Use the multi-step detergent-enzyme method to construct decellularized canine carotid artery allograft and cross-link it by EDC with the weight ratio of decellularized artery to EDC 1:1 and 1:2. Evaluate the biocompatibility of it by the cytotoxical MTT test and the rat subdermal bury test.

[Experimental study of compatibility between chondrocytes and allogenic cartilage microparticle acellular tissue matris in vitro].

Objective: To construct tissue engineered cartilage using cartilage microparticle acellular tissue matrix (CMACTM) as scaffold.

Methods: To determine the content of hydroxyproline, glycosaminoglycan and DNA of CMACTM prepared from sheep's articular cartilage with multistep enzymic method, and to analyze CMACTM with gross observation, histology and scanning electron microscopy. Allogenic chondrocytes were mixed with CMACTM and cultured in vitro from 0 to 35 days.

Tissue-engineered vessel strengthens quickly under physiological deformation: application of a new perfusion bioreactor with machine vision.

In order to develop a patent tissue-engineered blood vessel that grossly resembles native tissue, required culture times in most studies exceed 8 weeks. For the sake of shortening the maturation period of the constructs, we have used deformation as the basic index for mechanical environment control. A new bioreactor with a machine vision identifier was developed to accurately control the deformation of the construct during the perfusion process.

Applying informatics in tissue engineering.

Objective: To facilitate tissue engineering strategies determination with informatics tools.

Methods: Firstly, tissue engineering experimental data were standardized and integrated into a centralized database; secondly, we used data mining tools (e.g.

[Vascular anatomy and clinical applications of the distally based superficial sural artery island flap].

Objective: To document the vascular anatomy of the distally based superficial sural artery flap and to study the vascular anastomoses between the superficial sural artery and the septocutaneous perforators of the peroneal artery.

Methods: Ten fresh human cadavers were injected with lead oxide, gelatin and water. Twenty lags were then dissected and an overall map of the cutaneous vasculature was constructed.

[Transferring neurovascular rectus femoris muscle segment for treatment of facial paralysis].

Objective: To investigate a new technique for functional treatment of chronic facial paralysis.

Methods: Based on anatomy of intramuscular neurovascular structure in the rectus femoris muscle, 7 consecutive patients with facial paralysis were treated by using a technique of microsurgically free-transferring neurovascular rectus femoris muscle segment to the face in one-stage. Follow-ups were 10 to 24 months.

[The neurovascular anatomy and its clinical implication of the rectus femoris muscle].

Objective: The objective of this anatomic study was to investigate the intramuscular neurovascular configuration and to evaluate whether the muscle could be split into two functional units in transplantation.

Methods: Ten fresh cadavers and ten preserved cadavers were used in the study. A mixture of lead oxide, gelatin and water was injected to the femoral artery of the fresh cadaver.

[Effect of triton X-100 on preparing porcine thoracic aortas acellular matrix].

Objectives: To investigate the method of preparing porcine thoracic aortas acellular tissue matrix (ACTM) by trypsin, EDTA and Triton X-100 and to find the best concentration of X-100.

Methods: A total of 56 roots of fresh thoracic aortas (without adventitial tissue) from 80 kg-100 kg tame pigs were divided randomly into > groups, each containing 8 roots. Every vessel was put into a 50 ml centrifugal tube with a solution of 0.