[Effect of cisplatin on the expression of Pokemon gene: experiment with different human lung cancer cells].

Objective: To investigate the correlation between Pokemon gene and cisplatin mechanism.

Methods: Human lung adenocarcinoma cells of the lines A549 and AGZY83-a, human lung squamous carcinoma cells of the line HE-99, and human giant cell lung cancer cells of the line 95D were cultured and cisplatin was added into the medium. Other lung cancer cells of the above mentioned lines were cultured in the medium without cisplatin and were used as control groups.

[Suppression of RASSF1A gene on human esophageal carcinoma cells: experiments in vitro and in vivo].

Objective: To investigate the effect of Ras association domain family 1A (RASSF1A) gene, a new tumor suppressor gene (TSG), on tumorigenesis of human esophageal carcinoma cells.

Methods: pcDNA3.1 (+)-RASSF1A, a plasmid containing RASSF1A gene, and the blank plasmid pcDNA3.

[mRNA expression of RASSF1A in esophageal squamous cell carcinoma and clinical significance thereof].

Objective: To investigate the mRNA expression of the new tumor suppressor gene, RASSF1A, in esophageal squamous cell carcinoma and its biological value.

Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of RASSF1A in the tumor tissues, tissues near tumor, and normal tissues, all obtained during operation, from 66 esophageal squamous cell carcinoma patients.

Results: The deletion rates of RASSF1A mRNA expression were 42.