[Expression of PDCD5 in multiple myeloma and its relation with BCL-2].

Objective: To determine the expression of apoptosis related gene PDCD5 in multiple myeloma (MM), and to analyze the relation between PDCD5 and BCL-2.

Methods: The expressions of PDCD5 and BCL-2 protein and mRNA were determined by immunohistochemical staining method, flow cytometry (FCM) and reverse transcription polymerase chain reaction (RT-PCR) method in bone marrow mononuclear cells. We also analyzed the relation between PDCD5 and BCL-2.

[Screening the effective target sequences of laryngeal carcinoma related gene LCRG1].

Objective: To screen the effective target sequences of laryngeal carcinoma related gene LCRG1 using RNAi.

Methods: PCR site mutation method was used to reconstruct pSuper vector. Five pairs of siRNA sequences designed by siRNA software were annealed and inserted into the reconstructed pSuper vector.

[3 polymorphisms of gene GP IIb and the function of GP IIb T13959 G in the platelet transfusion refractoriness].

Objective: To investigate the relationship among 3 polymorphisms of GP IIb and the function of GP IIb T13959 G in the platelet transfusion refractoriness(PTR).

Methods: The 26th exon, the 30th exon and the 21st intron of gene GP IIb in 110 individuals were amplified by polymerase chain reaction (PCR), and the PCR products were analyzed with single-strand conformation polymorphism(SSCP) and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok I enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals.

[In vitro differentiation into megakaryocytes and generation of platelets from CD34+ cells of umbilical cord blood].

Objective: To induce hematopoietic progenitor/stem cells of umbilical cord blood to differentiate into mature megakaryocytes and platelets in vitro and to investigate the mechanism of production of platelets.

Methods: The CD34+ cells were sorted from umbilical cord blood by magnetic activated cell sorting (MACS) and then cultured in vitro with optimized medium to be differentiated into mature megakaryocytes and platelets. The cultured cells and the platelet-like particles were isolated from the culture and were checked by the fluorescence-activated cell sorter (FACS), immunohistochemistry assays, light microscope,electron microscope and platelet aggregation tests.

[Detection of anti-HCV in blood recipients with hematonosis].

Objective: To observe hepatitis C virus (HCV) infection in blood recipients with hematonosis, and to investigate the significance of anti-HCV detection in the patients.

Methods: Anti-HCV was detected by enzyme-linked immunosorbent assay (ELISA) in 176 hematonosis patients before blood transfusion, the result of anti-HCV was compared with control (417 cases), and 95 blood recipients were followed up for 6-12 months after the transfusion.

Results: The positive rate of anti-HCV was 5.