Early lethality of shRNA-transgenic pigs due to saturation of microRNA pathways.

RNA interference (RNAi) is considered as a potential modality for clinical treatment and anti-virus animal breeding. Here, we investigate the feasibility of inhibiting classical swine fever virus (CSFV) replication by short hairpin RNA (shRNA) in vitro and in vivo. We generate four different shRNA-positive clonal cells and two types of shRNA-transgenic pigs.

Effects of betamethasone on neuropathic pain in a rat spare nerve injury model.

The aim of the present study was to examine the effect of glucocorticoids on neuropathic pain using a rat spare nerve injury (SNI) model. Eighty rats were treated divided into the following groups: (i) a sham-operated group; (ii) a group subjected to SNI (S); (iii) a group subjected to SNI and administered 4 μg betamethasone intrathecally (D1); and (iv) a group subjected to SNI and administered 1 mg betamethasone at the site of nerve injury (D2). The mechanical withdrawal threshold (MWT) and thermal withdrawal duration (TWD) were measured 1 day before and the 1, 3, 7 and 14 days after SNI.

[Monitoring the change in CO concentration in combustion with tunable diode laser absorption spectroscopy].

In the present paper, the technology of tunable diode laser absorption spectroscopy (TDLAS) in conjunction with the open path multi-pass Herriot cell and the new-style detection method of auto-balanced detection combined with wavelength modulation technology were used, and the concentration of CO produced in combustion of alcohol blowtorch was measured. It was found in the measured result that the change in CO concentration in the flame of alcohol blowtorch presented a stated periodicity in the process of combustion and the average concentration of CO was calculated to be 49.4 (10(-6) ratio by volume).

[Second-harmonic detection with tunable diode laser absorption spectroscopy of CO and CO2 at 1.58 microm].

Tunable diode laser absorption spectroscopy has been applied in the fields of atmospheric chemistry and monitoring pollutant gases as a new method of measuring trace gases. The technique of remote sensing of CO and CO2 at 760 mm Hg pressure with tunable diode laser absorption spectroscopy in the near-infrared region is introduced. And the relationship between the second-harmonic spectrum of CO2 in Lorentzian line shape and the modulation amplitude is also presented.

[Selection of recombinant fowlpox virus coexpressing HIV-1 gag-gp120 and IL-6].

Objective: To construct the recombinant fowlpox virus (rFPV) coexpressing HIV-1 gag-gp120 and hIL-6.

Methods: The recombinant expressing plasmid pUTA-GE-IL6 was successfully constructed by inserting gag-gp120 gene and hIL-6 gene into the downstream of the combined promoter ATI-p7.5 and p7.