Determination of the Catalytic Activity of a Peroxidase-like Nanozyme and Differences among Layered Double Hydroxides with Different Anions and Cations.

Nanomaterials with enzyme-like activity, namely, nanozymes, have been widely used as substitutes for natural enzymes, and they show excellent potential for application in many fields, such as biotechnology, environmental chemistry, and medicine. Layered double hydroxides (LDHs) are inorganic nanomaterials with adjustable compositions, simple preparation methods, and low costs and are some of the most promising candidate materials for the preparation of nanozymes. Here, we studied the syntheses and peroxidase-like activities of LDHs with four anions and four cations.

Green synthesis and characterization of gold nanoparticles and their application for the rapid detection of glycyrrhizin with immunochromatographic strips.

In this study, a facile and environmentally friendly synthesis process was proposed without regular chemical additives. We successfully synthesized spherical gold nanoparticles (AuNPs) coated with glycyrrhizin (GL) by using GL as both a reductant and a stabilizer to reduce chloroauric acid. The obtained NPs were approximately 35 nm in size.

Effect of dietary oridonin supplementation on growth performance, gut health, and immune response of broilers infected with .

Background: The effects of dietary supplementation of oridonin (ORI) on growth performance, cecal microbiota, epithelium development and antioxidant and immune parameters of broilers infected with were studied. A total of 300 1-d-old male chicks were selected and divided into 5 trial groups (6 replicates of 10 chickens): 1) nonchallenge control chicks (), 2) chicks treated with Salmonella Challenged Control (), 3) chicks treated with challenge and 50 mg/kg ORI (O), 4) chicks treated with challenge and 80 mg/kg ORI (O), and 5) chicks treated with challenge and 100 mg/kg ORI (O).

Results: The results showed that had no effect on the feed intake (FI), body weight gain (BWG) or feed conversion ratio (FCR) of broilers compared with the values measured for the CON group ( > 0.

Effects of oridonin on immune cells, Th1/Th2 balance and the expression of BLys in the spleens of broiler chickens challenged with Salmonella pullorum.

The aim of this study was to investigate the effects of oridonin (ORI) on the immune cells, Th/Th balance and the expression of B lymphocyte stimulator (BLys) in the spleens of broilers infected with Salmonella pullorum. In a completely randomized design, 300 one-day-old AA male broilers were divided to 5 treatments. The groups included a noninfection control (CON) group receiveed a basal diet; a S.

Effects of dietary supplementation with oridonin on the growth performance, relative organ weight, lymphocyte proliferation, and cytokine concentration in broiler chickens.

Background: The present study was conducted to investigate the effects of oridonin (ORI) on growth performance, relative organ weight, lymphocyte proliferation, phagocytic function of neutrophils, and cytokine concentration in broiler chickens. A total of 240 one-day-old Arbor Acres male broilers were randomly assigned to four treatments with six replicate pens of 10 broiler chickens per pen. Broiler chickens were fed diets based on four levels of dietary ORI (0, 50, 80 and 100 mg/kg) for a 42-d feeding trial.

Natural Killer Group 2A Expressed on Both Peripheral CD3CD56NK Cells and CD3CD8T Cells Plays a Pivotal Negative Regulatory Role in the Progression of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.

To explore the role of surface receptors natural killer group 2A (NKG2A) and natural killer group 2D (NKG2D) on CD3CD8T cells and CD3CD56NK cells in the progression of hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF), we measured the expression of NKG2A and NKG2D on the surface of these 2 types of circulating cells by flow cytometry in 3 groups. One group consists of 36 patients with chronic hepatitis B (CHB), another one consists of 22 patients with HBV-related ACLF, and the last one has 12 normal controls (NC). The experimental result indicated that there was no significant difference in the proportion of CD3CD8T cells in total lymphocytes between the 3 groups.

Isolation and identification of ten metabolites of breviscapine in rat urine.

Breviscapine, is the total flavonoid components (the content of scutellarin > or =85%) extracted from the dried whole plant of Erigeron breviscapus (VANT.) HAND.-MAZZ, and its preparations are generally used in the clinic for the treatment of cerebral and cardio-vascular diseases in China.

Two major urinary metabolites of scutellarin in rats.

Urinary metabolites of scutellarin, the main effective constituent of breviscapine, a cerebrovascular and cardiovascular drug consisting of total flavonoids of Erigeron breviscapus, were investigated in rats. Two major metabolites were isolated from the urine of rats following oral administration of scutellarin and identified as scutellarein 6,7-di- O-beta-D-glucuronide (M1) and scutellarein (M2), respectively, on the basis of chemical and spectroscopic evidence. M1 was reported as a metabolite of scutellarin for the first time.

Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization.

It is often essential to focus the study on the small-size domains of large proteins in eukaryotic cells in the post-genomic era, but the low expression level, insolubility, and instability of the domains have been continuing to hinder the massive purification of domain peptides for structural and biological investigation. In this work, a highly efficient expression and purification system based on a small-size fusion partner GB1 and histidine tag was utilized to solve these problems. Two vectors, namely pGBTNH and pGBH, were constructed to improve expression and facilitate purification.

Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G.

Structural formation of segments plays pivotal roles in protein folding and stability, but how the segment influences the structural ensemble remains elusive. We engineered two hybrid proteins by replacing the central helical segment of immunoglobulin G binding domain of streptococcal protein G with an alpha-helix or beta(2)-strand element of a structural homologue, the immunoglobulin G binding domain of streptococcal protein L. The results show that substitution by the alpha-helical sequence retains a folded structure predominantly with a three-stranded beta-sheet but slightly destabilizes the compact ensemble, while substitution by the beta(2)-strand sequence completely destroys the structural formation.

[Gene mapping of a pedigree with autosomal dominant congenital cataract].

Objective: To map the gene for autosomal dominant congenital cataract (ADCC) in a Chinese family.

Methods: Blood samples were collected from 14 members of this family. Linkage analysis was carried out using short tandem repeat polymorphism (STRP) in close proximity to genes and loci previously reported involving in human cataract.

[Autosomal dominant congenital nuclear cataract caused by a deletion mutation in the beta A1-crystallin gene].

Objective: To identify the genetic defect causing automosal dominant congenital cataracts (ADCC) with nuclear opacities in a Chinese pedigree.

Methods: Linkage analysis was carried out with the short tandem repeat polymorphisms flanking the candidate genes. Mutation analysis of the candidate gene in the critical region was performed to detect the potential mutation.