The Genomic Characterization of Enterovirus D68 from 2011 to 2015 in Beijing, China.

A retrospective surveillance study on enterovirus D68 was performed in Beijing, China, following the largest and most widespread EV-D68 infection, which occurred in the USA. From January 2011 to July 2015, EV-D68 was identified in 12 individuals with respiratory infections in Beijing, China. The phylogenetic relationships based on the genomic sequence alignment showed that there were two lineages circulating in Beijing from 2011 to 2015.

[Response characteristics of soil water use patterns by different plants to precipitation in rocky mountainou areas.].

Water-use characteristics of plants are important for vegetation restoration in shallow earth-rock mountain area. In this study, soil and plant samples of Platycladus orientalis and corn were collected after rainfall events in Yingwugou watershed of Dan River to analyze the signatures of oxygen isotopes and the response of water use patterns to precipitation using stable isotope technology. The results showed that there were different response characteristics of the soil water utilization to precipitation between P.

Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR.

Objective: The aim of this study was to determine the etiology and prevalence of pediatric CAP in Beijing using a real-time polymerase chain reaction (PCR) technique.

Methods: Between February 15, 2011 and January 18, 2012, 371 pediatric patients with CAP were enrolled at Beijing Children's Hospital. Sixteen respiratory viruses and two bacteria were detected from tracheal aspirate specimens using commercially available multiplex real-time reverse transcription PCR (RT-PCR) kits.

[Rapid identification of Beijing measles vaccine virus and wild virus by multiplex real-time fluorescent PCR].

Objective: To identify wild measles virus and vaccine virus by detection nucleic acid of clinical samples from measles patients with immunization history circulating in Beijing through multiplex real-time fluorescent PCR technology.

Methods: From July 2011 to February 2012, 10 throat swabs and 15 urine specimens were collected from 16 suspected measles patients who were 8 - 9 months old infants with immunization history in Beijing. The specificity of multiplex real-time fluorescent PCR was firstly tested by measles vaccine virus, wild virus and other respiratory virus.

[Study on the Fast Testing Strategy for identifying the wild poliovirus I].

Objective: To explore the Fast Testing Sstrategy (FTS) for wild poliovirus I (WP1).

Methods: Epidemiological investigations were carried out on 671 students from WP1 epidemic areas in China. A set of real time RT-PCR assays, including panenterovirus testings (PE) assay, poliovirus serotypings (PS) assay and the assay distinguishing wild strain from vaccine strain of poliovirus I (DWV) were introduced into the screening program for WPV1 to replace the conventional RT-PCR, recommended by the China National Polio Laboratory (GNPL).

[Study on the prevalence of acute flaccid paralysis and hand foot mouth disease in Beijing 2006 - 2008].

Objective: To study the prevalence of acute flaccid paralysis (AFP) and hand foot mouth disease (HFMD) in Beijing, from 2006 - 2008.

Methods: Data on AFP and HFMD was analyzed epidemiologically, during 2006 - 2008 in Beijing. All the specimens from AFP cases were isolated and identified by RD and L20B cell and all of non-polio enterovirus (NPEV) cases were assayed by HFMD real-time PCR kit.

[Herd immunity against new influenza A (H1N1) in pre-vaccinated residents aged over 5 years in Beijing].

Objective: To explore the herd immunity against influenza A (H1N1) in pre-vaccinated residents aged over 5 years, and therefore to provide data for vaccination policies in high risk populations.

Methods: From October to December 2009, Beijing CDC conducted a serum survey of the novel influenza A (H1N1) in the local residents, stratified in 10 age groups between 5 years to over 60 years, without H1N1 vaccination history and disease history. Hemagglutination inhibition (HI) assays were performed at Beijing CDC.

[Comparison of genotype characteristics between the circulating mumps virus strain in Beijing area and the vaccine strain].

Objective: To compare the genetic characteristics of mumps virus strain circulating in Beijing with vaccine strain and to preliminarily analysis the reasons of vaccine ineffectiveness.

Methods: The following methods were used: Isolation and identification of the mumps virus which had been circulating in Beijing, immunization history analysis, SH gene sequence analysis and comparison genotype homology with reference strains and analysis of the key amino acid sites of HN variation.

Results: In 38 mumps cases that virus had been isolated from, another seven cases were IgM negative.

[Molecular typing of Neisseria meningitidis serogroup C strains with pulsed field gel electrophoresis in China].

Objective: To study the characteristics of epidemiology and molecular typing on Neisseria meningitidis serogroup C strains associated with outbreaks of Anhui province and sporadic cases in China, using pulsed field gel electrophoresis (PFGE).

Methods: 212 Neisseria meningitidis serogroup C strains were isolated from invasive meningococcal cases, close contacts and healthy carriers, including 48 strains from Anhui province with 38 strains associated with serogroup C outbreaks. PFGE were performed by genomic DNA digestion with Nhe I restriction enzyme.

The molecular characterization of serogroup C Neisseria meningitidis strains circulating in Beijing.

The aim of this study was to characterize the molecular features of serogroup C Neisseria meningitidis strains circulating in Beijing, China. Twenty out of 23 strains belonged to ST 4821. The causative serosubtype for meningococcal meningitis was P1.

[Surveillance on pathogens of meningococcal meningitis in Beijing, 2005].

Objective: To study the pathogens of meningococcal meningitis (MM) in Beijing, 2005.

Methods: Blood and cerebrospinal fluid specimens from MM patients were detected by polymerase chain reaction. Bacterial strains were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing.

Laboratory confirmation of a suspicious meningococcal meningitis death case.

A suspicious meningococcal meningitis death case was reported to the Beijing CDC. The blood specimen was analyzed via multi-PCR and MLST. 6 isolates from close contacts were analyzed via PFGE and MLST.