G-protein-coupled receptor 41 in cardiomyocytes: Effect of butyrate on intracellular Ca and sarcomere shortening.

G-protein-coupled receptor 41 (GPR41) is a Gα-coupled receptor activated by short-chain fatty acids (SCFAs). Here, we tested that GPR41 is also expressed in cardiomyocytes and exerts a direct negative inotropic effect when activated by SCFA butyrate. Primary cardiomyocytes were isolated from wild-type (WT) and GPR41 knockout (GPR41) adult mice and intracellular Ca concentration and cell shortening were measured using the IonOptix system.

Improving outcomes in intracerebral hemorrhage through microglia/macrophage-targeted IL-10 delivery with phosphatidylserine liposomes.

Intracerebral hemorrhage (ICH) remains the most lethal type of stroke, and effective clinical therapies that can speed up hematoma resolution after ICH are still lacking. While the beneficial effects of IL-10 on ICH recovery have been demonstrated, the clinical translation of IL-10 requires effective delivery methods by which sufficient IL-10 can be delivered to ICH-affected regions in the brain. Here we report the use of a phosphatidylserine (PS) liposome (PSL)-based nanoparticle system for microglia/macrophage-targeted delivery of IL-10 in ICH.

High expression of FABP4 in colorectal cancer and its clinical significance.

Objectives: To investigate the relationship between the fatty acid-binding protein 4 (FABP4) and colorectal cancer (CRC).

Methods: Using an enzyme-linked immunosorbent assay (ELISA), we measured the expression of FABP4 in plasma of 50 patients who underwent surgery for CRC from October 2017 to May 2018 and 50 healthy controls. The content of the visceral fat area (VFA) as seen with abdominal computed tomography (CT) scanning was measured by ImageJ software.

FABP4 promotes invasion and metastasis of colon cancer by regulating fatty acid transport.

Background: The prognosis of colon cancer is poor for metastasis, while the mechanism, especially adipocytes related, is not yet clear. The purpose of this study is to determine the effects of fatty acid binding protein 4 (FABP4), a transporter for lipids, on colon cancer progression.

Methods: The distribution of lipids and FABP4 was tested in the colon cancer tissues and adjacent normal tissues, and their relationship was also verified in vitro.

Intraarticular Injection of Allogenic Mesenchymal Stem Cells has a Protective Role for the Osteoarthritis.

Background: Researchers initially proposed the substitution of apoptotic chondrocytes in the superficial cartilage by injecting mesenchymal stem cells (MSCs) intraarticularly. This effect was termed as bio-resurfacing. Little evidence supporting the treatment of osteoarthritis (OA) by the delivery of a MSC suspension exists.

[Imaging reasons for periprosthetic femoral fractures during the operation of total hip arthroplasty with anatomic prosthesis].

Objective: To explore the imaging reasons for periprosthetic femoral fractures during the operation of total hip arthroplasty with anatomic prosthesis.

Methods: The fracture group consisted of 7 cases with periprosthetic femoral fracture (PPFF) and the non-fracture group 21 cases without PPFF during the operation of total hip arthroplasty (THA) among the 144 cases of primary THA with anatomic prosthesis. The preoperative plain films of hip joint were taken to calibrate the Sharp's angle, centre edge (CE) angle, femoral neck shaft angle, femoral anteversion angle of neck, bowing angle of proximal femoral shaft part, width of narrowest part in proximal femoral shaft.

Improvement of intertrochanteric bone quality in osteoporotic female rats after injection of polylactic acid-polyglycolic acid copolymer/collagen type I microspheres combined with bone mesenchymal stem cells.

Purpose: Osteoporosis mainly involves cancellous bone, and the spine and hip, with their relatively high cancellous bone to cortical bone ratio, are severely affected. Studies of bone mesenchymal stem cells (BMSCs) from osteoporotic patients and animal models have revealed that osteoporosis is often associated with reduction of BMSCs' proliferation and osteogenic differentiation. Our aim was to test whether polylactic acid-polyglycolic acid copolymer(PLGA)/collagen type I(CoI) microspheres combined with BMSCs could be used as injectable scaffolds to improve bone quality in osteoporotic female rats.

[Surgical treatment for degenerative lumbar scoliosis associated with spinal stenosis].

Objective: To study surgical techniques for degenerative lumbar scoliosis associated with lumbar stenosis and evaluate their clinical significane.

Methods: Thirty-two patients with degenerative lumbar scoliosis associated with spinal stenosis were treated by techniques of posterior lumbar interbody fusion or posterolateral fusion and pedicle screws. There were 18 male and 14 female with 56.

[The etiologies and prevention of patellar instability after un-resurfaced total knee arthroplasty].

Objective: To investigate the etiologies and prevention of patellar instability after total knee arthroplasty (TKA).

Methods: From September 1997 to April 2005, 94 patients (105 knees) underwent TKA without patellar resurfacing. All of them were osteoarthritis.

Augmentation of pedicle screw fixation strength using an injectable calcium sulfate cement: an in vivo study.

Study Design: An in vivo landrace model of cement augmentation of pedicle screw was established, and axial pull-out tests and histological analysis were performed.

Objective: To investigate the long-term in vivo biomechanical performance of pedicle screws augmented with calcium sulfate cement.

Summary Of Background Data: Little information is available on the long-term biomechanical performance of pedicle screws augmented with calcium sulfate cement in vivo.

[Change in the joint line after total knee arthroplasty].

Objective: To investigate the change in joint line after knee arthroplasty.

Methods: A total of 43 patients had 25 primary cruciate-retaining total knee replacements (Link Gemini MKII) and 25 rotational knee replacements (Link Endo-Model Rotational Knee System) sacrificing collateral ligaments and cruciate ligaments. Lateral radiographs were made both preoperatively and postoperatively.

[The manufacture of pelvic mirror physical model using rapid prototyping].

Objective: To study the manufacture of pelvic mirror physical model using rapid prototyping and the accuracy of the models.

Methods: The pelvic CT images were acquired by spiral CT thin slice scanning, and the slicing data of pelvis were created from these CT images by computer image-processing. Then the pelvic mirror physical model was manufactured using rapid prototyping.