Effects of sampling strategies and DNA extraction methods on eDNA metabarcoding: A case study of estuarine fish diversity monitoring.

Environmental DNA (eDNA) integrated with metabarcoding is a promising and powerful tool for species composition and biodiversity assessment in aquatic ecosystems and is increasingly applied to evaluate fish diversity. To date, however, no standardized eDNA-based protocol has been established to monitor fish diversity. In this study, we investigated and compared two filtration methods and three DNA extraction methods using three filtration water volumes to determine a suitable approach for eDNA-based fish diversity monitoring in the Pearl River Estuary (PRE), a highly anthropogenically disturbed estuarine ecosystem.

Mitochondrial genome of provides insights into the phylogeny of Labeoninae.

is an endemic species living in the karst regions of South China. In the present study, we firstly performed the complete mitogenome sequence of (Cyprinidae: Labeoninae). Results showed that the mitogenome was 16,596 bp in length and contained 37 genes (i.

Characterization and phylogenetic analysis of the complete mitochondrial genome of (Teleost: Cyprinidae).

In this study, we assembled the complete mitochondrial genome of based on high-throughput Illumina sequencing. The complete mitochondrial genome of was 16,598 bp in size. It was made up of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region.

Characterization of the complete mitochondrial genome of (Teleost: Balitoridae) and its phylogenetic analysis.

In this study, we assembled the complete mitochondrial genome of , based on high-throughput Illumina sequencing. The complete mitochondrial genome of was 16,576 bp in size. It was made up of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region.

Stochastic determination of the spatial variation of potentially pathogenic bacteria communities in a large subtropical river.

Understanding the composition and assembly mechanism of waterborne pathogen is essential for preventing the pathogenic infection and protecting the human health. Here, based on 16S rRNA sequencing, we investigated the composition and spatial variation of potentially pathogenic bacteria from different sections of the Pearl River, the most important source of water for human in Southern China. The results showed that the potential pathogen communities consisted of 6 phyla and 64 genera, covering 11 categories of potential pathogens mainly involving animal parasites or symbionts (AniP), human pathogens all (HumPA), and intracellular parasites (IntCelP).