[Viable Bacteria Assay of by RT-qPCR Measurement of Gene Expression Levels: Establishment and Application of a New Method].

Objective: To establish a viable bacteria assay for () by assessing the gene expression, and to develop accordingly a rapid and novel testing method for clinical precision treatment.

Methods: Viable bacteria count was determined in bacterial cultures. The transcriptional expression level of (), the conserved gene that encodes cholesterol-α-glucosyltransferase (CGT) in , was measured by RT-PCR.