Chitosan induced cold tolerance in by regulating photosynthesis, antioxidant performance, and chloroplast ultrastructure.

Introduction: Cold stress is the primary factor that limits the growth and development of in the Tibetan Plateau, China. Chitosan (CTS) has been recognized for its ability to enhance agricultural production and tolerance to stress.

Methods: This study examined the effect of treating seedlings under cold stress with chitosan.

Genome-Wide Identification and Characterization of the Gene Family in , and Expression Patterns under Abiotic Stress and Hormone Treatments.

is a key model plant for studying legume plants, particularly alfalfa (), due to its well-defined genetic background. Plant-specific () genes play various roles in plant growth and development, abiotic stress, and hormone responses. However, limited information is available on research in .

Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.

Background: Kobreisa littledalei, belonging to the Cyperaceae family is the first Kobresia species with a reference genome and the most dominant species in Qinghai-Tibet Plateau alpine meadows. It has several resistance genes which could be used to breed improved crop varieties. Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) is a popular and accurate gene expression analysis method.

Insights into the Impact of Trans-Zeatin Overproduction-Engineered on Alfalfa ( L.) Tolerance to Drought Stress.

Plant growth-promoting rhizobacteria have been shown to enhance plant tolerance to drought stress through various mechanisms. However, there is limited research on improving drought resistance in alfalfa by genetically modifying PGPR to produce increased levels of cytokinins. Herein, we employed synthetic biology approaches to engineer two novel strains of capable of overproducing trans-Zeatin and investigated their potential in enhancing drought tolerance in alfalfa.

Genome assembly and multi-omic analyses reveal the mechanisms underlying flower color formation in Torenia fournieri.

Torenia fournieri Lind. is an ornamental plant that is popular for its numerous flowers and variety of colors. However, its genomic evolutionary history and the genetic and metabolic bases of flower color formation remain poorly understood.

A 3-Ketoacyl-CoA Synthase 10 () Homologue from Alfalfa Enhances Drought Tolerance by Regulating Cuticular Wax Biosynthesis.

Cuticular wax, forming the first line of defense against adverse environmental stresses, comprises very long-chain fatty acids (VLCFAs) and their derivatives. 3-Ketoacyl-CoA synthase (KCS) is a rate-limiting enzyme for VLCFA biosynthesis. In this study, we isolated , a gene from alfalfa, and analyzed the effect of gene expression on wax production and drought stress in transgenic plants.

Photosynthesis, Water Status and K/Na Homeostasis of Responding to Salinity.

Soil salinization is one of the most serious abiotic stresses restricting plant growth. Buffalograss is a C perennial turfgrass and forage with an excellent resistance to harsh environments. To clarify the adaptative mechanisms of buffalograss in response to salinity, we investigated the effects of NaCl treatments on photosynthesis, water status and K/Na homeostasis of this species, then analyzed the expression of key genes involved in these processes using the qRT-PCR method.

Osmotic stress-induced lignin synthesis is regulated at multiple levels in alfalfa (Medicago sativa L.).

Alfalfa is an important forage crop. Yield and quality are frequently threatened by extreme environments such as drought and salt stress. As a component of the cell wall, lignin plays an important role in the abiotic stress response, the mechanisms of which have not been well clarified.

Identification of Competing Endogenous RNAs (ceRNAs) Network Associated with Drought Tolerance in with Rhizobium Symbiosis.

Drought, bringing the risks of agricultural production losses, is becoming a globally environmental stress. Previous results suggested that legumes with nodules exhibited superior drought tolerance compared with the non-nodule group. To investigate the molecular mechanism of rhizobium symbiosis impacting drought tolerance, transcriptome and sRNAome sequencing were performed to identify the potential mRNA-miRNA-ncRNA dynamic network.

Iron nanoparticles induced the growth and physio-chemical changes in Kobresia capillifolia seedlings.

Iron nanoparticles (NPs) priming is known to affect the seed germination and seedling growth in many plants. However, whether it has an important role in stimulating the growth of perennial Qinghai-Tibet Plateau plants remains unclear. In this study, the effects of seed priming with different concentrations of nFeO and FeCl (10, 50, 100, 500, and 1000 mg L) on seed germination, plant growth, photosystem, antioxidant enzyme activities, root morphology, and biomass distribution of Kobresia capillifolia were evaluated under laboratory conditions.

Proteomic Analysis of Alfalfa ( L.) Roots in Response to Nodulation and Salt Stress.

(1) Background: Alfalfa is an important legume forage throughout the world. Although alfalfa is considered moderately tolerant to salinity, its production and nitrogen-fixing activity are greatly limited by salt stress. (2) Methods: We examined the physiological changes and proteomic profiles of alfalfa with active nodules (NA) and without nodules (NN) under NaCl treatment.

Distinct rhizosphere soil responses to nitrogen in relation to microbial biomass and community composition at initial flowering stages of alfalfa cultivars.

In the long-term growth process, alfalfa rhizosphere forms specific microbiome to provide nutrition for its growth and development. However, the effects of different perennial alfalfa cultivars on changes in the rhizosphere soil characteristics and microbiome are not well understood. In this study, 12 perennial alfalfa cultivars were grown continuously for eight years.

Effects of MiR-214-3p Regulation of SERCA2a Expression on Contractility of Cardiomyocytes in Heart Failure Model.

This study aimed to explore the targeted regulation of microRNA-214-3p (miR-214-3p) on sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase 2a (SERCA2a) and its mechanism on heart failure (HF). In this study, a rat model of HF was established by injecting isoproterenol to detect the changes in heart function. Then the primary rat cardiomyocytes were extracted and cultured.

Green nanopriming: responses of alfalfa (Medicago sativa L.) seedlings to alfalfa extracts capped and light-induced silver nanoparticles.

The application of nanotechnology in agriculture can remarkably improve the cultivation and growth of crop plants. Many studies showed that nanoparticles (NPs) made plants grow more vigorously. Light can make NPs aggregated, leading to the reduction of the NPs toxicity.

MDP25 mediates the fine-tuning of microtubule organization in response to salt stress.

Microtubules are dynamic cytoskeleton structures playing fundamental roles in plant responses to salt stress. The precise mechanisms by which microtubule organization is regulated under salt stress are largely unknown. Here, we report that Arabidopsis thaliana MICROTUBULE-DESTABILIZING PROTEIN 25 (MDP25; also known as PLASMA MEMBRANE-ASSOCIATED CATION-BINDING PROTEIN 1 (PCaP1)) helps regulate microtubule organization.

Ming-Mu-Di-Huang-Pill Activates SQSTM1 via AMPK-Mediated Autophagic KEAP1 Degradation and Protects RPE Cells from Oxidative Damage.

Oxidative stress and diminished autophagy in the retinal pigment epithelium (RPE) play crucial roles in the pathogenesis of age-related macular degeneration (AMD). Enhancing autophagy has recently been identified as an important strategy to protect RPE cells from oxidative damage. Ming-Mu-Di-Huang-Pill (MMDH pill) is a traditional herbal medicine used to treat AMD, and its molecular mechanism is not well understood.

Analysis of risk factors for early death in patients with acute promyelocytic leukaemia treated with arsenic trioxide.

To date, no specific studies have evaluated early death (ED) in patients with acute promyelocytic leukaemia (APL) homogeneously treated with arsenic trioxide induction therapy and investigated according to the white blood cell (WBC) count at onset. Such patients were retrospectively analysed in this study, including 314 patients with a WBC count ≤ 10 × 10/L (standard-risk (SR) group) and 144 with a WBC count > 10 × 10/L (high-risk (HR) group). The baseline clinical characteristics and risk factors for ED were compared between the two groups.

Experimental and Theoretical Study of the Effects of Rare Earth Elements on Growth and Chlorophyll of Alfalfa ( L.) Seedling.

Rare earth elements (REEs) of low concentration are usually beneficial to plant growth, while they are toxic at high concentrations. The effects of treatment with lanthanum (La) (10 and 20 μM), cerium (Ce) (10 and 20 μM), and terbium (Tb) (10 and 20 μM) on seedling growth of alfalfa ( L.), which is one of the most important perennial leguminous forages in the world, were studied.

Composition and Protein Precipitation Capacity of Condensed Tannins in Purple Prairie Clover (.).

This study aimed to determine the concentration and composition of condensed tannins (CT) in different tissues of purple prairie clover (PPC; Vent.) at different maturities and to determine their protein-precipitating capacity. The compositions of CT were elucidated after thiolysis with benzyl mercaptan followed by high-performance liquid-chromatography (HPLC) and H-C heteronuclear single quantum coherence (HSQC) NMR spectroscopy.

Addressing the challenge of cold stress resilience with the synergistic effect of Rhizobium inoculation and exogenous melatonin application in Medicago truncatula.

Cold stress is an adverse environmental condition that limits the growth and yield of leguminous plants. Thus, discovering an effective way of ameliorating cold-mediated damage is important for sustainable legume production. In this study, the combined use of Rhizobium inoculation (RI) and melatonin (MT) pretreatment was investigated in Medicago truncatula plants under cold stress.

Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath.

The rhizosheath, a layer of soil grains that adheres firmly to roots, is beneficial for plant growth and adaptation to drought environments. Switchgrass is a perennial C4 grass which can form contact rhizosheath under drought conditions. In this study, we characterized the microbiomes of four different rhizocompartments of two switchgrass ecotypes (Alamo and Kanlow) grown under drought or well-watered conditions via 16S ribosomal RNA amplicon sequencing.

Exploration of the Effect and Mechanism of , and in the Treatment of AMD Based on Network Pharmacology and in vitro Experimental Verification.

Purpose: The aim of this study was to observe the mechanism of (FL), (RRP) and (PL) in treating age-related macular degeneration (AMD) based on network pharmacology and biological experiments.

Methods: Bioactive compounds, potential targets of FL, RRP and PL, and genes related to AMD, were acquired from public databases. Functional and pathway enrichment analyses of the core targets were conducted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

LKB1/AMPKα signaling pathway and mitochondrial fission/fusion dynamics regulate apoptosis induced by 3-chlorpropane-1,2-diol in HEK293 cells.

Mitochondrial dynamics and bioenergetics are considered play pivotal roles in the maintenance of mitochondrial function and cell viability. During the widely distributed food contaminant 3-chlorpropane-1,2-diol (3-MCPD) induced nephrotoxicity, mitochondrial morphology and function were impaired, but the specific mechanism responsible for the process has not been fully elucidated. In the present study, using an in vitro human embryonic kidney 293 (HEK293) cell culture model, the role of LKB1/AMPK pathway and mitochondrial fission and fusion dynamics in 3-MCPD-induced cell apoptosis was investigated by using the AMPK inhibitor dorsomorphin and mitochondrial division inhibitor 1 (Mdivi-1), respectively.

Functional Analysis of the Phosphate Transporter Gene From .

Phosphorus is one of the essential macronutrients required by plant growth and development, but phosphate resources are finite and diminishing rapidly because of the huge need in global agriculture. In this study, 11 genes were found in the () family of . Seven genes of the family were available by qRT-PCR.