Distribution-based maximum likelihood estimation methods are preferred for estimating Salmonella concentration in chicken when contamination data are highly left-censored.

Salmonella is a common chicken-borne pathogen that causes human infections. Data below the detection limit, referred to as left-censored data, are frequently encountered in the detection of pathogens. The approach of handling the censored data was regarded to affect the estimation accuracy of microbial concentration.

A Meta-Analysis and Systematic Review of Response to Sanitizer Treatments.

Listeria monocytogenes is a ubiquitous organism that can be found in food-related environments, and sanitizers commonly prevent and control it. The aim of this study is to perform a meta-analysis of L. monocytogenes response to sanitizer treatments.

Machine learning approach for predicting single cell lag time of Salmonella Enteritidis after heat and chlorine treatment.

The importance of single-cell variability is increasingly prominent with the developments in foodborne pathogens modeling. Traditional predictive microbiology model cannot accurately describe the growth behavior of small numbers of cells due to individual cell heterogeneity. The objective of the present study was to develop predictive models for single cell lag times of Salmonella Enteritidis after heat and chlorine treatment.

Antibiotic Resistance of Typhimurium Monophasic Variant 1,4,[5],12:i:-in China: A Systematic Review and Meta-Analysis.

Antibiotic resistance in is a global public health problem. serovar 1,4,[5],12:i:- (. 1,4,[5],12:i:-), a monophasic variant of Typhmurium, is one of the leading serovars in several countries.

The Prevalence and Epidemiology of in Retail Raw Poultry Meat in China: A Systematic Review and Meta-Analysis.

Foodborne disease caused by is an important public health concern worldwide. Animal-based food, especially poultry meat, is the main source of human salmonellosis. The objective of this study was to evaluate the prevalence and epidemiology of contamination in raw poultry meat commercialized in China.

Probabilistic model for estimating Listeria monocytogenes concentration in cooked meat products from presence/absence data.

A quantitative probabilistic model was developed to estimate the concentration of Listeria monocytogenes in cooked meat products based on presence/absence data and an assumed zero-inflated distribution, i.e. zero-inflated Poisson (ZIP) or zero-inflated Poisson lognormal (ZIPL) distribution.

The prevalence of Listeria monocytogenes in meat products in China: A systematic literature review and novel meta-analysis approach.

Meat products are commonly regarded as one of the main sources of human listeriosis caused by Listeria monocytogenes. The objective of this study was to estimate the prevalence of L. monocytogenes in a range of meat products from 24 different Chinese regions by using meta-analysis of literature data and a novel sensitivity analysis approach.

[Progress in detection and modeling of quorum sensing molecules of foodborne pathogens].

Quorum sensing (QS) plays a major role in the outbreak mechanism of foodborne diseases caused by food poisoning and food spoilage. QS affects the formation of cell membrane and pathogenicity ofpathogenic bacteria. Through the in-depth understanding of QS molecules of food-borne pathogens, we describe here the types of signal molecules produced by Gram-negative and Gram-positive bacteria, and the differences in QS molecules.

[Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney lines].

Objective: To investigate the expression and function of PKD1 and PKD2 in different kidney tissues and cell lines.

Methods: Immunoprecipitation, Western blotting, In situ hybridization and immunohistochemical staining methods were used to observe the expression of PKD1 mRNA and PKD2 mRNA and their protein abundance in different kidney tissues and cell lines.

Results: Coordinate expressions of PKD1 and PKD2 were found in all kidney tissues and cell lines.

Role of keratinocyte growth factor in the pathogenesis of autosomal dominant polycystic kidney disease.

Background: Previous studies have shown that the expression and distribution of keratinocyte growth factor (KGF), also known as FGF-7 (fibroblast growth factor-7) or HBGF-7 (heparin-binding growth factor-7), may be implicated in kidney cyst formation and expansion. However, there are no data on KGF expression in human autosomal dominant polycystic kidney disease (ADPKD) tissue, and it is unknown whether it affects ADPKD cyst-lining epithelial cell epithelial cell proliferation.

Methods: The expression and distribution of KGF and KGF receptor (KGFR) mRNA in ADPKD cystic and normal kidney tissues were examined using quantitative real-time polymerase chain reaction (PCR) and in situ hybridization.

Mutation analysis of autosomal dominant polycystic kidney disease genes in Han Chinese.

Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in two genes, PKD1 and PKD2. The complexity of these genes, particularly PKD1, has complicated genetic screening, though recent advances have provided new opportunities for amplifying these genes. In the Han Chinese population, no complete mutational analysis has previously been conducted across the entire span of PKD1 and PKD2.

[Gene diagnosis of autosomal dominant polycystic kidney disease type 2 using microsatellite DNA tightly linked to polycystic kidney disease gene 2].

Objective: To use microsatellite DNA tightly linked to polycystic kidney disease gene 2 in the gene diagnosis of autosomal dominant polycystic kidney disease type 2.

Methods: Microsatellite DNA of D4S1534, D4S1542, D4S1563,D4S2460 and D4S423 were amplified with PCR and the fragments of products were analyzed by capillary electrophoresis and Genescan and Genotyper software, and then gene diagnosis of the pedigrees was made by linkage analysis.

Results: Three families were found to be linked to PKD2 in 20 families.

[Mutation detection of PKD2 gene in Chinese by denaturing high-performance liquid chromatograph].

Objective: To detect the mutations of autosomal dominant polycystic kidney disease gene 2(PKD2)in Chinese.

Methods: The white blood cell genomic DNA from patients of 94 Chinese autosomal dominant polycystic kidney disease(ADPKD) pedigrees was isolated and amplified by polymerase chain reaction(PCR). The PCR products were analyzed by denaturing high-performance liquid chromatography(DHPLC).

[Preparation and characterization of monoclonal antibody against N-terminal domain of polycystin 1].

Aim: To prepare and identify monoclonal antibody (mAb) against N-terminal domain of polycystin 1.

Methods: Total RNA was extracted from kidney tissue of a healthy man. Gene sequence encoding polycystin 1 N-terminal domain was amplified by one-step RT-PCR.