Optical computing metasurfaces: applications and advances.

Integrated photonic devices and artificial intelligence have presented a significant opportunity for the advancement of optical computing in practical applications. Optical computing technology is a unique computing system based on optical devices and computing functions, which significantly differs from the traditional electronic computing technology. On the other hand, optical computing technology offers the advantages such as fast speed, low energy consumption, and high parallelism.

Annular multi-focal-phase mask multiplexing based large depth of field imaging by interferenceless coded aperture correlation holography.

Extending depth-of-field (DOF) of the imaging system without modifying the structure and sacrificing imaging performances of the optical system is of great significance to broaden the capability and application of the imaging system. In this paper, the interferenceless coded aperture correlation holography(I-COACH) is developed to be a large-depth incoherent imaging system by employing an annular multi-focal coded phase mask (AM-CPM). Based on the analyses of axial defocus characteristics in I-COACH, the defocus compensation function is defined, the AM-CPM is designed and multiplexed on the system optical pupil, which plays the role of a gradual lens.

Computational adaptive holographic fluorescence microscopy based on the stochastic parallel gradient descent algorithm.

Optical aberrations introduced by sample or system elements usually degrade the image quality of a microscopic imaging system. Computational adaptive optics has unique advantages for 3D biological imaging since neither bulky wavefront sensors nor complicated indirect wavefront sensing procedures are required. In this paper, a stochastic parallel gradient descent computational adaptive optics method is proposed for high-efficiency aberration correction in the fluorescent incoherent digital holographic microscope.

One-Dimensional STED Microscopy in Optical Tweezers.

Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems.

Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System.

Recent advances in the design and measurement capabilities of optical tweezers instruments, and especially the combination with multi-color fluorescence detection, have accommodated a dramatic increase in the versatility of optical trapping. Quadruple (Q)-trap optical tweezers are an excellent example of such an advance, by providing three-dimensional control over two constructs and thereby enabling for example DNA-DNA braiding. However, the implementation of fluorescence detection in such a Q-trapping system poses several challenges: (1) since typical samples span a distance in the order of tens of micrometers, it requires imaging of a large field of view, (2) in order to capture fast molecular dynamics, fast imaging with single-molecule sensitivity is desired, (3) in order to study three-dimensional objects, it could be needed to detect emission light at different axial heights while keeping the objective lens and thus the optically trapped microspheres in a fixed position.

High-quality interferenceless coded aperture correlation holography with optimized high SNR holograms.

Motivated by the key role of point spread function in an imaging system, we propose an interferenceless coded aperture correlation holographic (I-COACH) technology with low speckle and high energy efficiency annular sparse coded phase mask (CPM) as system pupil to improve imaging performance. In the proposed method, a modified Gerchberg-Saxton (GS) algorithm is proposed to obtain a low speckle and high energy efficiency annular sparse CPM and to suppress speckle and increase the intensity of the holograms. Therefore, the randomly distributed amplitude in the bandwidth of the GS algorithm is replaced by the annular amplitude to determine the spatial position, and the band-limited random phase and quadratic phase are used as the initial phase to approximately meet band-limited conditions; meanwhile, in the iterative process of the algorithm, appropriate constraints are imposed on the information within and outside the band limit.

Optimized reconstruction with noise suppression for interferenceless coded aperture correlation holography.

A modified nonlinear reconstruction technique with a noise modulation parameter is proposed for interferenceless coded aperture correlation holography (I-COACH), and thus the signal-to-noise ratio of a reconstructed image is improved without sacrifice of the field of view and temporal resolution of the system. In order to obtain the optimal reconstructed image, no-reference structural sharpness (NRSS) is introduced as the evaluation metric of reconstructed image quality during nonlinear reconstruction. On the other hand, the noise modulation function is built in order to analyze the effect of phase on noise when the amplitude of the point spread hologram and object hologram is unity of 1.

Adaptive optics via self-interference digital holography for non-scanning three-dimensional imaging in biological samples.

Three-dimensional imaging in biological samples usually suffers from performance degradation caused by optical inhomogeneities. Here we proposed an approach to adaptive optics in fluorescence microscopy where the aberrations are measured by self-interference holographic recording and then corrected by a post-processing optimization procedure. In our approach, only one complex-value hologram is sufficient to measure and then correct the aberrations, which results in fast acquisition speed, lower exposure time, and the ability to image in three-dimensions without the need to scan the sample or any other element in the system.

Self-interference compressive digital holography with improved axial resolution and signal-to-noise ratio.

Fresnel incoherent correlation holography (FINCH) was proposed to break the barrier of spatial incoherent digital holographic imaging and show the potential of super-resolution imaging preferences. We developed FINCH as a compressive sensing modality and reconstruction procedure as an inverse problem in order to realize 3D tomographic imaging. Improved axial resolution is obtained via compressive reconstruction.

Real-time extended interface automata for software testing cases generation.

Testing and verification of the interface between software components are particularly important due to the large number of complex interactions, which requires the traditional modeling languages to overcome the existing shortcomings in the aspects of temporal information description and software testing input controlling. This paper presents the real-time extended interface automata (RTEIA) which adds clearer and more detailed temporal information description by the application of time words. We also establish the input interface automaton for every input in order to solve the problems of input controlling and interface covering nimbly when applied in the software testing field.

Improved ant algorithms for software testing cases generation.

Existing ant colony optimization (ACO) for software testing cases generation is a very popular domain in software testing engineering. However, the traditional ACO has flaws, as early search pheromone is relatively scarce, search efficiency is low, search model is too simple, positive feedback mechanism is easy to produce the phenomenon of stagnation and precocity. This paper introduces improved ACO for software testing cases generation: improved local pheromone update strategy for ant colony optimization, improved pheromone volatilization coefficient for ant colony optimization (IPVACO), and improved the global path pheromone update strategy for ant colony optimization (IGPACO).

Incoherent off-axis Fourier triangular color holography.

We present a new method for recording off-axis digital Fourier holograms of three-dimensional objects under spatially incoherent illumination. The method is implemented by modifying the optical configuration of triangular interferometer. The recording properties and 3D reconstruction ability of the proposed method are investigated theoretically and experimentally.