Naphpyrones A-H, Antibacterial Aromatic Polyketides Isolated from the A3(2)/ ΔH3.

, a common foodborne pathogen, has a close association with agriculture and food. With the rapid emergence and widespread dissemination of antimicrobial resistance, efforts have been directed toward developing and studying new antimicrobial compounds to inhibit the growth of and other foodborne pathogens, thereby preventing contamination and ensuring food safety. Herein, we reported eight new aromatic polyketides, naphpyrones A-H (-), from the heterologous expression strain A3(2)/ ΔH3.

Heterologous Expression of Type II PKS Gene Cluster Leads to Diversified Angucyclines in J1074.

Heterologous expression has emerged as an effective strategy in activating cryptic gene clusters or improving yield. Eight compounds were successfully obtained by heterologous expression of the type II PKS gene cluster derived from marine sp. HDN155000 in the chassis host J1074.

Novel antifungal talaroenamine L with unusual leptosphaeronyl moiety from marine-derived sp. HDN17-428 via HDACi elicitation.

Vorinostat elicitation of the deep-sea fungus sp. HDN17-428 resulted in the isolation of one new compound, talaroenamine L () with unusual leptosphaeronyl moiety and three known compounds (-). Structures of - were confirmed by extensive NMR, HRESIMS and OR calculations.

Mitigating Capacity and Voltage Decay in Li-Rich Cathode Via Dual-Phase Design.

High-capacity O3-type lithium-rich manganese-based (LRM) materials exhibit significant structural instability and severe voltage decay, which limit their practical applications. In contrast, the O2-type LRM materials demonstrate remarkable structural stability despite offering lower capacity. In this study, a composite material, O3@O2-LRM is designed, by coating the main structure of O3-type LRM with a minor amount of O2-type LRM to combine the high capacity of the O3 phase with the superior stability of the O2 phase.

Polyketide Derivatives from the Mangrove-Derived Fungus sp. HDN15-312.

Four new polyketides, namely furantides A-B (-), talamin E () and arugosinacid A (), and two known polyketides were obtained from the mangrove-derived fungus sp. HDN15-312 using the One Strain Many Compounds (OSMAC) strategy. Their chemical structures, including configurations, were elucidated by detailed analysis of extensive NMR spectra, HRESIMS and ECD.

Biosynthesis of Atypical Angucyclines Unveils New Ring Rearrangement Reactions Catalyzed by Flavoprotein Monooxygenases.

Six new angucycline structures, including spirocyclione A (), which contains an unusual oxaspiro[5.5]undecane architecture, and its ring-A-cleaved product spirocyclione B (), were discovered by heterologous expression of a type II polyketide biosynthetic gene cluster captured from a marine actinomycete strain sp. HDN155000.

Multifunctional Cross-Linking Composite Binder Enables the Stable Performance of Si-Based Anodes for High-Energy-Density Lithium-Ion Batteries.

The intrinsic volumetric stress during cycling is the main obstacle for developing Si-based materials as high-energy-density lithium-ion battery anodes. Elastic binders have been demonstrated as an efficient approach to alleviate the stress of Si. Herein, we design a tough 3D hard/soft polymeric network (LPTS) using lithiated poly(acrylic acid), silk sericin, and highly branched tannic acid.

Sorbremnoids A and B: NLRP3 Inflammasome Inhibitors Discovered from Spatially Restricted Crosstalk of Biosynthetic Pathways.

Crosstalk-oriented chemical evolution of natural products (NPs) is an efficacious strategy for generating novel skeletons through coupling reactions between NP fragments. In this study, two NOD-like receptor protein 3 (NLRP3) inflammasome inhibitors, sorbremnoids A and B ( and ), with unprecedented chemical architectures were identified from a fungus . Compounds and exemplify rare instances of hybrid NPs formed via a major facilitator superfamily (MFS)-like enzyme by coupling reactive intermediates from two separate biosynthetic gene clusters (BGCs), and .

Discovery, Total Synthesis, and Anti-Inflammatory Evaluation of Naturally Occurring Naphthopyrone-Macrolide Hybrids as Potent NLRP3 Inflammasome Inhibitors.

Numerous clinical disorders have been linked to the etiology of dysregulated NLRP3 (NACHT, LRR, and PYD domain-containing protein 3) inflammasome activation. Despite its potential as a pharmacological target, modulation of NLRP3 activity remains challenging. Only a sparse number of compounds have been reported that can modulate NLRP3 and none of them have been developed into a commercially available drug.

Exploring the Diverse Landscape of Fungal Cytochrome P450-Catalyzed Regio- and Stereoselective Dimerization of Diketopiperazines.

Dimeric indole-containing diketopiperazines (di-DKPs) are a diverse group of natural products produced through cytochrome P450-catalyzed C-C or C-N coupling reactions. The regio- and stereoselectivity of these reactions plays a significant role in the structural diversity of di-DKPs. Despite their pivotal role, the mechanisms governing the selectivity in fungi are not fully understood.

Phenylhydrazone Alkaloids from the Deep-Sea Cold Seep Derived Fungus HDN21-0307.

Alkaloids with a phenylhydrazone architecture are rarely found in nature. Four unusual phenylhydrazone alkaloids named talarohydrazones A-D (-) were isolated from the deep-sea cold seep derived fungus HDN21-0307 using the one strain-many compounds (OSMAC) approach and MS/MS-based molecular networking (MN) combined with network annotation propagation (NAP) and the unsupervised substructure annotation method MS2LDA. Their structures were elucidated by spectroscopic data analysis, single-crystal X-ray diffraction, and quantum chemical calculations.

Antiviral and cytotoxic indole diterpenoids from the antarctic sponge-derived fungus HDN15-152.

One new indole diterpenoid, ascandinine T (), and three known analogues (-) were isolated from an Antarctic sponge-derived fungus HDN15-152. The structures, including absolute configurations, were established based on NMR, HRESIMS, and electronic circular dichroism (ECD) calculations. All isolated compounds were tested for antiviral and anticancer activity.

Leveraging machine learning for prediction of antibiotic resistance genes post thermal hydrolysis-anaerobic digestion in dairy waste.

Anaerobic digestion holds promise as a method for removing antibiotic resistance genes (ARGs) from dairy waste. However, accurately predicting the efficiency of ARG removal remains a challenge. This study introduces a novel appproach utilizing machine learning to forecast changes in ARG abundances following thermal hydrolysis-anaerobic digestion (TH-AD) treatment.

Evolution-Based Discovery of Polyketide Acylated Valine from a Cytochalasin-Like Gene Cluster in HDN13430.

Utilizing a gene evolution-oriented approach for gene cluster mining, a cryptic cytochalasin-like gene cluster () in Antarctic-derived HDN13430 was identified. Compared with the canonical cytochalasin biosynthetic gene clusters (BGCs), the gene cluster lacks the key α,β-hydrolase gene. Heterologous expression of the gene cluster led to the discovery of a new compound, slamysin (), characterized by an -acylated amino acid structure and demonstrating weak anti- activity.

Cyclo-diphenylalanine production in Aspergillus nidulans through stepwise metabolic engineering.

Cyclo-diphenylalanine (cFF) is a symmetrical aromatic diketopiperazine (DKP) found wide-spread in microbes, plants, and resulting food products. As different bioactivities continue being discovered and relevant food and pharmaceutical applications gradually emerge for cFF, there is a growing need for establishing convenient and efficient methods to access this type of compound. Here, we present a robust cFF production system which entailed stepwise engineering of the filamentous fungal strain Aspergillus nidulans A1145 as a heterologous expression host.

Antibacterial p-terphenyl and α‑pyrone derivates isolated from the marine-derived actinomycete Nocardiopsis sp. HDN154086.

Assisted by OSMAC strategy, one new p-terphenyl and two new α‑pyrone derivates, namely nocarterphenyl I (1) and nocardiopyrone D-E (2-3), were obtained and characterized from the marine sediment-derived actinomycete Nocardiopsis sp. HDN154086. The structures of these compounds were determined on the basis of MS, NMR spectroscopic data and single-crystal X-ray diffraction.

Impacts of irrigation with treated livestock wastewater on the accumulation characteristic of ARGs in the farmland soil: a case study in Hohhot, China.

Irrigation with treated livestock wastewater (TWW) is a promising strategy for reusing resources. However, TWW irrigation might introduce antibiotic resistant genes (ARGs) into the soil, posing environmental risks associated with antibiotic resistance. This study focuses on investigating the influence of irrigation amounts and duration on the fate of ARGs and identifies key factors driving their changes.

Extending the Structural Diversity of Labdane Diterpenoids from Marine-Derived Fungus sp. HDN151403 Using Heterologous Expression.

Heterologous biosynthesis has become an effective means to activate fungal silent biosynthetic gene clusters (BGCs) and efficiently utilize fungal genetic resources. Herein, thirteen labdane diterpene derivatives, including five undescribed ones named talarobicins A-E (-), were discovered via heterologous expression of a silent BGC () in . Their structures with absolute configurations were elucidated using extensive MS and NMR spectroscopic methods, as well as electronic circular dichroism (ECD) calculations.

Hollow Structure CoS/3D-TiCT MXene Composite for Separator Modification of Lithium-Sulfur Batteries.

The commercial application of lithium-sulfur (Li-S) batteries has faced obstacles, including challenges related to low sulfur utilization, structural degradation resulting from electrode volume expansion, and migration of polysulfide lithium (LiPSs). Herein, CoS/3D-TiCT composites with three-dimensional (3D) multilayered structures are used as separator modification materials for Li-S batteries to solve these problems. The multilevel layered structure of CoS/3D-TiCT establishes an efficient electron and Li transfer path, alleviates the volume change during the battery charge-discharge process, and enhances the stability of the structure.

Activation of a Silent Polyketide Synthase SlPKS4 Encoding the C-Methylated Isocoumarin in a Marine-Derived Fungus HDN13-430.

Coumarins, isocoumarins and their derivatives are polyketides abundant in fungal metabolites. Although they were first discovered over 50 years ago, the biosynthetic process is still not entirely understood. Herein, we report the activation of a silent nonreducing polyketide synthase that encodes a C-methylated isocoumarin, similanpyrone B (), in a marine-derived fungus HDN13-430 by heterologous expression.

A novel antimycin analogue antimycin A2c, derived from marine Streptomyces sp., suppresses HeLa cells via disrupting mitochondrial function and depleting HPV oncoproteins E6/E7.

Aims: Novel antimycin alkaloid antimycin A2c (AE) was isolated from the culture of a marine derived Streptomyces sp. THS-55. We elucidated its chemical structure by extensive spectra and clarified the specific mechanism in HPV infected-cervical cancer.

In situ synthesis of VS/TiCT MXene composites as modified separators for lithium-sulfur battery.

Lithium-sulfur (Li-S) batteries are regarded as highly prospective energy storage devices. However, problems such as low sulfur utilization, poor cycle performance, and insufficient rate capability hinder the commercial development of Li-S batteries. Three-dimensional (3D) structure materials have been applied to modify the separator of Li-S batteries to suppress the diffusion of lithium polysulfides (LiPSs) and inhibit the transmembrane diffusion of Li.

Design, synthesis, and biological evaluation of novel penindolone derivatives as potential inhibitors of hemagglutinin-mediated membrane fusion.

Development and design of anti-influenza drugs with novel mechanisms is of great significance to combat the ongoing threat of influenza A virus (IAV). Hemagglutinin (HA) is regarded as a potential target for the therapy of IAV. Our previous research led to the discovery of penindolone (PND), a new diclavatol indole adduct, as an HA targeting leading compound exhibited anti-IAV activity.