Emission Onset Time-Adjustable Chemiluminescent Gold Nanoparticles with Ultrastrong Emission for Smartphone-Based Immunoassay of Severe Acute Respiratory Syndrome Coronavirus 2 Antigen.

Recently, our group reported a chemical timer approach to manipulate the onset time of chemiluminescence (CL) emission. However, it is still in the proof-of-concept stage, and its analytical applications have not been explored yet. Nanomaterials have merits of good catalytic effect, large specific surface area, good biocompatibility, and ease of self-assembly, which are ideal for constructing analytical-interfaces for bioassays.

A chemical timer approach to delayed chemiluminescence.

Although the onset time of chemical reactions can be manipulated by mechanical, electrical, and optical methods, its chemical control remains highly challenging. Herein, we report a chemical timer approach for manipulating the emission onset time of chemiluminescence (CL) reactions. A mixture of Mn, NaHCO, and a luminol analog with HO produced reactive oxygen species (ROS) radicals and other superoxo species (superoxide containing complex) with high efficiency, accompanied by strong and immediate CL emission.

Imaging the oxygen wave with a single bioluminescent bacterium.

We developed a capability of a monolayer of bioluminescent (BL) bacteria for spatiotemporally visualizing the heterogeneous distribution and dynamic evolution of interfacial oxygen concentration, resulting in the discovery of spontaneous and stochastic oxygen waves at the interface between the substrate and an undisturbed, apparently still solution. Wild type bacteria, , spontaneously emit light during the native metabolism processes, , bioluminescence. The emission intensity is sensitively regulated by oxygen concentration.

Rational design of functional materials guided by single particle chemiluminescence imaging.

Chemiluminescence (CL) functionalized materials have found tremendous value in developing CL assays for clinical assays and point-of-care tests. To date, the design and optimization of these materials have mainly relied on conventional trial-and-error procedures in which the ensemble performance is evaluated using conditional experiments. Here we have built an optical microscope to acquire the CL emission from single magnetic-polymer hybrid microbeads functionalized with luminol analogues, and to access the CL kinetics of each individual particle.

Potential-Resolved Multicolor Electrochemiluminescence of N-(4-Aminobutyl)-N-ethylisoluminol/tetra(4-carboxyphenyl)porphyrin/TiO Nanoluminophores.

Most electrochemiluminescence (ECL) studies involve single luminophore with a unique emission process, which severely limits its applications. Recently, multicolor ECL has attracted considerable interests. Herein, we report a novel nanoluminophore prepared by coating 5,10,15,20-tetrakis(4-carboxyphenyl)-porphyrin (TCPP) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) on the surface of TiO nanoparticles (TiO-TCPP-ABEI), which exhibited unique potential-resolved multicolor ECL emissions using HO and KSO as coreactants in an aqueous solution.