Alloanti-D induction in a rare RhD variant (DBT-2) case: Insights from serological and molecular biological testing.

Background: The RhD antigen is the most immunogenic within the Rh blood group system， playing a pivotal role in clinic. The D variant phenotype is a rare occurrence, characterized by low expression of the D antigen or partial deletion of the RhD antigen on the surface of red blood cells (RBCs). For individuals with the D variant, transfusion with RhD-negative blood is crucial for ensuring transfusion safety.

2-Mercaptoethanol (2-ME)-based IATs or Polybrene method mitigates the interference of daratumumab on blood compatibility tests.

Objectives: Treating red blood cells (RBCs) with dithiothreitol (DTT) is a wildly-recommended to overcome the interference of the daratumumab (DARA) with blood compatibility testing. Nevertheless, DTT can be hard to obtain in the clinical laboratory, while its use in routine practice may be time-consuming. In the following study, we explored the feasibility of using a commercial 2-mercaptoethanol (2-ME) working solution or the time-saving Polybrene method to mitigate DARA interference.

The serological and genetic characterization of CisAB blood group in a Chinese family.

Background: The rare CisAB blood genotype results in the inheritance of both the A and B blood types from a single parent. Several CisAB blood type phenotypes have been characterized that differ in their serological reactions and in the activities of the gene-encoded blood group A and B transferases. In this study, we conducted serological and genetic analyses of a Chinese family with four CisAB-carrying members.

[Application of microfluidic chip analytical systems in ABO genotyping].

Limitations of polyacrylamide gel or agarose gel electrophoretic methods in genotyping research affect the interpreting of detection results. In order to develop a simple and reliable method for appraising results of ABO genotyping detection, the microfluidic chip analysis system was established by using microfluidic chip to replace the gel electrophoresis and combining with multiplex-PCR-RFLP technique. 150 blood samples were tested by this microfluidic chip analysis system with multiplex-PCR-RFLP technique to evaluate its stability and accuracy.

[ABO genotyping of Han population in Beijing].

The aim of this study was to establish a diagnostic method for ABO genotyping and to investigate the distribution of ABO genotype in Beijing Han population so as to understand the distribution characteristics and regularity of ABO genotype. An ABO genotyping method was established by using multiplex-PCR-RFLP and PCR-SSP techniques, and the ABO allele frequency in Beijing Han population was investigated. The results showed that A102, O1 and B allele were more common genes in Beijing Han individuals.