Differential expression of circular RNAs in interleukin 6-promoted osteogenic differentiation of human stem cells from apical papilla.

Introduction: Studies have shown that interleukin 6 (IL-6) can regulate stem cell osteogenic differentiation; however, the exact mechanism is not clear. Circular RNAs (circRNAs) are closed circular non-coding RNAs that are involved in the process of stem cell osteogenic differentiation. Therefore, the purpose of this present study was to investigate the effect of IL-6 treatment on osteogenic differentiation of human apical tooth papillae stem cells (hSCAPs), and to detect the difference in circRNA expression using gene microarray technology.

The bioceramic sealer iRoot SP promotes osteogenic differentiation of human stem cells from apical papilla via miR-141-3p/SPAG9/MAPK signalling pathway.

Aim: The premixed bioceramic sealer iRoot SP that is widely used clinically has been reported to kill bacterial biofilms and promote osteogenic differentiation of human stem cells from the apical papilla (hSCAPs). Although miR-141-3p has been substantiated to be involved in the osteogenic process, the underlying mechanisms remain unclear. The aim of this study was to investigate the role of miR-141-3p in osteogenic differentiation and underlying mechanisms of iRoot SP-treated hSCAPs.

Effects of Atmospheric Pressure Plasma in the Treatment of Experimental Periodontitis in Beagle Dogs.

Objective: The specific objective of this study was to evaluate the effects of atmospheric pressure plasma (APP) in the treatment of experimental periodontitis in Beagle dogs.

Methods: The APP jet was diagnosed using optical emission spectroscopy and laser-induced fluorescence spectroscopy. Six Beagles received stainless steel ligatures to establish experimental periodontitis model.

Transcatheter arterial chemoembolization is safe and effective for patients with late-stage or recurrent oral carcinoma.

Objective: We reported the long-term outcomes of transcatheter chemoembolization (TACE) for patients with late-stage or recurrent oral carcinoma.

Methods: This retrospective study enrolled 18 patients with late-stage or recurrent oral carcinoma between December 2015 and April 2021. The tumor-feeding artery was catheterized, and cisplatin/oxaliplatin and 5-FU/raltitrexed were infused with embolization using polyvinyl alcohol or gelatin sponge.

Effects of iRoot SP on osteogenic differentiation of human stem cells from apical papilla.

Background: Research shows that nano-bioceramics can modulate the differentiation of dental stem cells. The novel ready-to-use calcium-silicate-based root-canal sealer iRoot SP is widely used in root filling. Accordingly, the aim of this study was to evaluate the effects of iRoot SP on proliferation and osteogenic differentiation in human stem cells from the apical papilla (hSCAPs).

Chondrocyte Co-cultures with the Stromal Vascular Fraction of Adipose Tissue in Polyhydroxybutyrate/Poly-(hydroxybutyrate-co-hydroxyhexanoate) Scaffolds: Evaluation of Cartilage Repair in Rabbit.

Chondral defects are challenging to repair because of the poor self-healing capacity of articular cartilage. The aim of this study was to compare and investigate the cartilage regeneration of stromal vascular fraction (SVF) cells and adipose-derived stem cells (ASCs) co-cultured with chondrocytes seeding on scaffolds composed of polyhydroxybutyrate (PHB)/poly-(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx). In this study, the cellular morphologies and proliferation capabilities on scaffolds were evaluated.

Effects of miRNA-342-3p in modulating Hedgehog signaling pathway of human umbilical cord mesenchymal stem cells by down-regulating Sufu.

Objective: Previously, we have shown that miRNA-342-3p was increased during osteogenic differentiation of human umbilical mesenchymal stem cells (hUCMSCs) via regulating the sonic hedgehog (Shh) pathway. In this study, our objective is to further investigate the role of miRNA-342-3p in activation of Shh pathway by targeting suppressor of fused protein (Sufu), a suppressor of transcriptional factor Gli, as well as the potential interaction with transforming growth factor beta (TGF-β) signaling pathway during osteogenic induction of hUCMSCs.

Materials And Methods: HUCMSCs that stable overexpression or knockdown of miRNA-342-3p were established by infection with lentiviral vectors.

Tanshinone Suppresses Arecoline-Induced Epithelial-Mesenchymal Transition in Oral Submucous Fibrosis by Epigenetically Reactivating the p53 Pathway.

Oral submucous fibrosis (OSF) induced by chewing of the areca nut has been considered to be a precancerous lesion with a high probability of developing oral squamous cell carcinoma. Tanshinone (TSN) is the main component extracted from , a traditional Chinese medicine, which was found to have diverse pharmacological effects, such as anti-inflammatory and antitumor. In the current study, we aimed to identify the inhibitory effects and the underlying mechanism of TSN on OSF progress.

Physical properties and hydration behavior of a fast-setting bioceramic endodontic material.

Background: To investigate the physical properties and the hydration behaviour of the fast-setting bioceramic iRoot FS Fast Set Root Repair Material (iRoot FS) and three other endodontic cements.

Methods: iRoot FS, Endosequence Root Repair Material Putty (ERRM Putty), gray and white mineral trioxide aggregate (G-MTA & W-MTA), and intermediate restorative material (IRM) were evaluated. The setting time was measured using ANSI/ADA standards.

Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals.

Introduction: The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model.

Methods: Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks.

Effects of non-equilibrium plasma in the treatment of ligature-induced peri-implantitis.

Aim: To evaluate the effects of non-equilibrium plasma in the treatment of ligature-induced peri-implantitis in beagle dogs.

Materials And Methods: Six beagles received 12 implants installed in the position of the fourth mandibular premolars. Ligature-induced peri-implantitis was initiated at 3 months post-implantation.

Effect of a combination of torsional and cyclic fatigue preloading on the fracture behavior of K3 and K3XF instruments.

Introduction: The purpose of this study was to evaluate the effect of various degrees of cyclic fatigue on torsional failure and torsional preloading on the cyclic fatigue life of heat-treated K3XF nickel-titanium (NiTi) instruments (SybronEndo, Orange, CA).

Methods: The mean number of cycles until failure (Nf) of K3XF and K3 NiTi instruments was examined in a 3-point bending apparatus with a 7-mm radius and 45° curve. Torque and distortion angles at failure of new instruments and instruments stressed to 25%, 50%, and 75% of the Nf were measured according to ISO 3630-1.

In vitro cytotoxicity of calcium silicate-containing endodontic sealers.

Introduction: The cytotoxicity of 2 novel calcium silicate-containing endodontic sealers to human gingival fibroblasts was studied.

Methods: EndoSequence BC (Brasseler, Savannah, GA), MTA Fillapex (Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brazil) and a control sealer (AH Plus; Dentsply DeTrey GmbH, Konstanz, Germany) were evaluated. Human gingival fibroblasts were incubated for 3 days both with the extracts from fresh and set materials in culture medium and cultured on the surface of the set materials in Dulbecco-modified Eagle medium.

Cultivation and identification of rat bone marrow-derived mesenchymal stem cells.

Bone marrow‑derived mesenchymal stem cells (BMSCs) have the potential to form a variety of mesenchymal tissue types, which are a source of cells for bone tissue engineering applications. The present study attempted to establish an effective and convenient method for culturing BMSCs. Total bone marrow cells, which were harvested from rat femurs, were cultured and BMSCs were selected and expanded through passaging in vitro.

Effect of long-term exposure to endodontic disinfecting solutions on young and old Enterococcus faecalis biofilms in dentin canals.

Introduction: The purpose of this study was to evaluate the antimicrobial activity on Enterococcus faecalis biofilms in dentin canals of short-term and long-term exposure to different endodontic disinfecting solutions by using a dentin infection model and confocal laser scanning microscopy.

Methods: Dentinal tubules in semi-cylindrical dentin blocks were filled with E. faecalis by centrifugation and incubated to form 1-day-old and 3-week-old biofilms.

Effect of modified nonequilibrium plasma with chlorhexidine digluconate against endodontic biofilms in vitro.

Introduction: Nonequilibrium plasma has been reported to effectively kill Enterococcus faecalis in endodontic biofilm compared with chlorhexidine digluconate (CHX). The purpose of this study was to evaluate the antimicrobial in vitro activity of modified nonequilibrium plasma with CHX against E. faecalis and multispecies biofilms on bovine dentin discs.

A three-dimensional finite element analysis for overdenture attachments supported by teeth and/or mini dental implants.

Purpose: The aim of this study was to establish the optimum design and attachment combination to support an overdenture with minimal stress and flexing produced in the alveolar bone surrounding any natural teeth and/or mini dental implants.

Materials And Methods: Twelve models were included in the study: the six main models (A, B, C, D, E, and F) were categorized according to the support designs of the overdenture prosthesis, and each model was further subdivided according to the attachment combinations into model 1: with Dalbo elliptic and/or O-ring attachments only and model 2: with flexible acrylic attachments. Vertical loads (35 N) and 17.

Evaluation of antibacterial effects by atmospheric pressure nonequilibrium plasmas against Enterococcus faecalis biofilms in vitro.

Introduction: The aim of this study was to evaluate the in vitro antibacterial activity by atmospheric pressure nonequilibrium plasmas (APNPs) as an effective approach against bacterial biofilms in root canal systems during endodontic therapy.

Methods: Sterile cover slips were placed into the wells of tissue culture plates to permit the formation of Enterococcus faecalis biofilms. Biofilms were treated for 5 minutes with APNPs or 2% chlorhexidine digluconate (CHX).

An in vitro investigation into retention strength and fatigue resistance of various designs of tooth/implant supported overdentures.

Previously, the choice of prosthetic implant-retained overdentures has depended on data from previous studies about the retention-fatigue strength of the attachment system selected. Little or no data have been available on the correlation between the attachment system selected and the overdenture support configuration. The purpose of the present study was to evaluate the retention force and fatigue resistance of three attachment systems and four support designs of overdenture prosthesis.

Bacterial-killing effect of atmospheric pressure non-equilibrium plasma jet and oral mucosa response.

Recently, plasma sterilization has attracted increasing attention in dental community for the atmospheric pressure non-equilibrium plasma jet (APNPs), which is driven by a kilohertz pulsed DC power, may be applied to the dental and oral diseases. However, it is still in doubt whether APNPs can effectively kill pathogenic bacteria in the oral cavity and produce no harmful effects on normal oral tissues, especially on normal mucosa. The aim of this study was to evaluate the bacterial-killing effect of APNPs in the biofilms containing a single breed of bacteria (Porphyromonas gingivalis, P.