Synthesis, cytotoxicity and HQSAR study of amides-fused isosteviol derivatives as potential anti-colorectal cancer agents.

A series of novel amide-fused isosteviol derivatives were designed and synthesized. Their cytotoxicities in vitro against HCT-116 cells were screened. The preliminary bioassays indicated that most of the title compounds exhibited noteworthy cytotoxicity.

Integrative metabolomics and proteomics reveal the effect and mechanism of Zi Qi decoction on alleviating liver fibrosis.

Liver fibrosis is a common progressive liver disease that can cause liver dysfunction and lead to serious complications. Zi Qi decoction (ZQ) is a traditional formulation that exerts pharmacological effects on the treatment of liver fibrosis. However, precise intervention mechanisms remain unclear.

Normalization of WISP1 circulating level and tissue expression following metabolic and bariatric surgery using rat model.

Wingless-type inducible signaling pathway protein-1 (WISP1) is a newly recognized adipokine, associated with obesity and type 2 diabetes (T2DM). This study aimed to investigate the effect of metabolic and bariatric surgery (MBS) on WISP1 circulating (serum) levels and tissue expression using rat models. We initially investigated whether WISP1 circulating levels were altered between the T2DM and normal rats.

Characteristic and stability changes of peanut oil body emulsion during the process of demulsification using heptanoic acid.

In this paper, the changes in oil body emulsion (OBE) during heptanoic acid demulsification (HD) were investigated from the macro and microscopic points of view. Specifically, the OBE particle size increased from 3.04 to 8.

Content-Aware Human Mobility Pattern Extraction.

Extracting meaningful patterns of human mobility from accumulating trajectories is essential for understanding human behavior. However, previous works identify human mobility patterns based on the spatial co-occurrence of trajectories, which ignores the effect of activity content, leaving challenges in effectively extracting and understanding patterns. To bridge this gap, this study incorporates the activity content of trajectories to extract human mobility patterns, and proposes acontent-aware mobility pattern model.

A Review on Traditional Processes and Laser Powder Bed Fusion of Aluminum Alloy Microstructures, Mechanical Properties, Costs, and Applications.

Due to its lightweight, high strength, good machinability, and low cost, aluminum alloy has been widely used in fields such as aerospace, automotive, electronics, and construction. Traditional manufacturing processes for aluminum alloys often suffer from low material utilization, complex procedures, and long manufacturing cycles. Therefore, more and more scholars are turning their attention to the laser powder bed fusion (LPBF) process for aluminum alloys, which has the advantages of high material utilization, good formability for complex structures, and short manufacturing cycles.

CARD11 regulates the thymic Treg development in an NF-κB-independent manner.

Introduction: CARD11 is a lymphoid lineage-specific scaffold protein regulating the NF-κB activation downstream of the antigen receptor signal pathway. Defective CARD11 function results in abnormal development and differentiation of lymphocytes, especially thymic regulatory T cells (Treg).

Method: In this study, we used patients' samples together with transgenic mouse models carrying pathogenic mutations from patients to explore their effects on Treg development.

Impact of tocilizumab on anti-CD19 chimeric antigen receptor T-cell therapy in B-cell acute lymphoblastic leukemia.

Background: Tocilizumab is commonly used for the management of chimeric antigen receptor (CAR) T-cell therapy-associated cytokine release syndrome (CRS). However, it remains unknown whether tocilizumab or its dosage affects the efficacy and safety of CAR T-cell therapy. The objective of this multicenter retrospective study was to explore the impact of tocilizumab on CAR T-cell therapy.

Demulsification of Emulsion Using Heptanoic Acid during Aqueous Enzymatic Extraction and the Characterization of Peanut Oil and Proteins Extracted.

Peanut oil body emulsion occurs during the process of aqueous enzymatic extraction (AEE). The free oil is difficult to release and extract because its structure is stable and not easily destroyed. Demulsification can release free oil in an oil body emulsion, so various fatty acids were selected for the demulsification.

Stretchable and recyclable gelatin Ionogel based ionic skin with extensive temperature tolerant, self-healing, UV-shielding, and sensing capabilities.

Fabricating sustainable ionic skin with multi-functional outstanding performances using biocompatible natural polymer-based ionogel is highly desired but remains a great challenge up to now. Herein, a green and recyclable ionogel has been fabricated by in-situ cross-linking of gelatin with a green bio-based multifunctional cross-linker of Triglycidyl Naringenin in ionic liquid. Benefiting from the unique multifunctional chemical crosslinking networks along with multiple reversible non-covalent interactions, the as-prepared ionogels exhibit high stretchability (>1000 %), excellent elasticity, fast room-temperature self-healability (>98 % healing efficiency at 6 min), and good recyclability.

CaMKIV mediates spine growth deficiency of hippocampal neurons by regulation of EGR3/BDNF signal axis in congenital hypothyroidism.

Congenital hypothyroidism (CH) will cause cognitive impairment in the condition of delayed treatment. The hippocampus is one of the most affected tissues by CH, in which the functional structures of hippocampal neurons manifest deficiency due to aberrant expression of effector molecules. The Ca/Calmodulin-dependent protein kinase, CaMKIV, is downregulated in the hippocampal neurons, influencing the growth of dendritic spines in response to CH.

Visible-light-mediated aerobic Ritter-type C-H amination of diarylmethanes using DDQ/-butyl nitrite.

A metal-free photocatalytic Ritter-type C-H amination of unactivated sp carbons using molecular oxygen as a terminal oxidant has been developed. By employing a co-catalytic system of 3-dichloro-4,5-dicyano-1,4-benzoquinone (DDQ) and -butyl nitrite (TBN), this novel strategy provides a low cost, sustainable and scalable way to synthesise a broad range of secondary amides in moderate to excellent yields under mild conditions.

Value of the prognostic nutritional index (PNI) in patients with newly diagnosed, CD5-positive diffuse large B-cell lymphoma: A multicenter retrospective study of the Huaihai Lymphoma Working Group.

Background: CD5-positive diffuse large B-cell lymphoma (DLBCL) is a clinically rare subtype of DLBCL with aggressive clinical manifestations and a poor prognosis. It has been demonstrated that the prognostic nutritional index (PNI), an indicator of nutritional status and systemic inflammation, is a significant prognostic factor for several types of lymphoma. The objective of this multicenter retrospective study was to explore the prognostic value of the PNI in patients with CD5-positive DLBCL.

Muscle-Mimetic Highly Tough, Conductive, and Stretchable Poly(ionic liquid) Liquid Crystalline Ionogels with Ultrafast Self-Healing, Super Adhesive, and Remarkable Shape Memory Properties.

Here, we report a simple method for preparing muscle-mimetic highly tough, conductive, and stretchable liquid crystalline ionogels which contains only one poly(ionic liquid) (PIL) in an ionic liquid via in situ free radical photohomopolymerization by using nitrogen gas instead of air atmosphere. Due to eliminating the inhibition caused by dissolved oxygen, the polymerization under nitrogen gas has much higher molecular weight, lower critical sol-gel concentration, and stronger mechanical properties. More importantly, benefiting from the unique loofah-like microstructures along with the strong internal ionic interactions, entanglements of long PIL chains and liquid crystalline domains, the ionogels show special optical anisotropic, superstretchability (>8000%), high fracture strength (up to 16.

Cold Atmospheric Plasma Boosts Virus Multiplication via EGFR(Tyr1068) Phosphorylation-Mediated Control on Cell Mitophagy.

Vaccination still remains as the most effective approach for preventing infectious diseases such as those caused by virus infection, with cell-based vaccine manufacturing being one flexible solution regarding the spectrum of infectious disorders it can prevent. Rapid cell-based virus propagation can enable high yield of vaccines against viral diseases that may offer critical values in the industry when handling emergent situations such as the ongoing viral disease pandemic. Through investigating the phenomenon and biological mechanism underlying redox-triggered cell survival towards enhanced viral particle production, this study explores novel strategies for improved yield of viral particles at a reduced cost to meet the increasing demand on cell-based vaccine manufacturing against viral diseases.

Experimental investigations and the modeling approach for CO solubility in aqueous blended amine systems of monoethanolamine, 2-amino-2-methyl-1-propanol, and 2-(butylamino)ethanol.

In this work, new CO solubility data on three types of aqueous amine blends were reported to complement existing databases. The experiments were conducted at temperatures of 313 K (absorption condition) and 363 K (desorption condition). The effect of the MEA concentration on the CO solubility in several amine blends at low CO partial pressure (8 to 50.

Dual Thermo-Responsive and Strain-Responsive Ionogels for Smart Windows and Temperature/Motion Monitoring.

In this work, a stretchable, dual thermo-responsive and strain-responsive ionogel has been synthesized by one-step photopolymerization. The obtained ionogel shows an ultrahigh stretchability (∼3000%), a high ionic conductivity (up to 3.1 mS/cm), and a good temperature tolerance (-40 to 300 °C).

Anti-PD-1 Therapy Enhances the Efficacy of CD30-Directed Chimeric Antigen Receptor T Cell Therapy in Patients With Relapsed/Refractory CD30+ Lymphoma.

Anti-CD30 CAR-T is a potent candidate therapy for relapsed/refractory (r/r) CD30+ lymphomas with therapy limitations, and the efficacy needed to be further improved. Herein a multi-center phase II clinical trial (NCT03196830) of anti-CD30 CAR-T treatment combined with PD-1 inhibitor in r/r CD30+ lymphoma was conducted. After a lymphocyte-depleting chemotherapy with fludarabine and cyclophosphamide, 4 patients in cohort 1 and 3 patients in cohort 2 received 10/kg and 10/kg CAR-T cells, respectively, and 5 patients in cohort 3 received 10/kg CAR-T cells combined with anti-PD-1 antibody.

Ultrastretchable, Adhesive, Fast Self-Healable, and Three-Dimensional Printable Photoluminescent Ionic Skin Based on Hybrid Network Ionogels.

Developing multifunctional stretchable ionic skin (I-Skin) to mimic the sensations of the human skin is of great interest and shows promising potential in wearable sensors and human-machine interfaces (HMIs). However, common ionogels prepared with small-molecule cross-linkers and single networks can hardly satisfy the requirements of adjustable mechanical properties, strong adhesion, fast self-healability, and good stability in extreme environments. Herein, an ultrastretchable (>10,000%), ultrastrong adhesive (>6.

Development and Validation of a Novel Prognostic Nomogram for CD5-Positive Diffuse Large B-Cell Lymphoma: A Retrospective Multicenter Study in China.

Background: CD5-positive diffuse large B-cell lymphoma (CD5+ DLBCL) is a rare subtype of DLBCL with invasive clinical features and poor prognosis. Current clinical variables based on prognostic systems for DLBCL are inadequate to accurately stratify the prognosis of CD5+ DLBCL.

Methods: A total of 195 CD5+ DLBCL patients were retrospectively recruited from nine centers in Huaihai Lymphoma Working Group.

Design and Evaluation of a Prescription Drug Monitoring Program for Chinese Patent Medicine based on Knowledge Graph.

Background: Chinese patent medicines are increasingly used clinically, and the prescription drug monitoring program is an effective tool to promote drug safety and maintain health.

Methods: We constructed a prescription drug monitoring program for Chinese patent medicines based on knowledge graphs. First, we extracted the key information of Chinese patent medicines, diseases, and symptoms from the domain-specific corpus by the information extraction.

Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways.

BACKGROUND Hepatic stellate cells (HSCs) play a vital role in hepatic fibrogenesis. Our recent clinical study indicated that the Zi Qi decoction, a Traditional Chinese Medicine formula, exhibited good efficacy in alleviating liver fibrosis, but the underlying mechanism remains elusive. MATERIAL AND METHODS Rats repeatedly injected with CCl₄ and cells stimulated with lipopolysaccharide were used as in vivo and in vitro models for liver fibrosis, respectively.

TRPV1 feed-forward sensitisation depends on COX2 upregulation in primary sensory neurons.

Increased activity and excitability (sensitisation) of a series of molecules including the transient receptor potential ion channel, vanilloid subfamily, member 1 (TRPV1) in pain-sensing (nociceptive) primary sensory neurons are pivotal for developing pathological pain experiences in tissue injuries. TRPV1 sensitisation is induced and maintained by two major mechanisms; post-translational and transcriptional changes in TRPV1 induced by inflammatory mediators produced and accumulated in injured tissues, and TRPV1 activation-induced feed-forward signalling. The latter mechanism includes synthesis of TRPV1 agonists within minutes, and upregulation of various receptors functionally linked to TRPV1 within a few hours, in nociceptive primary sensory neurons.