Identification of C-geranylated flavonoids from Paulownia catalpifolia Gong Tong fruits by HPLC-DAD-ESI-MS/MS and their anti-aging effects on 2BS cells induced by HO.

The fruits of Paulownia catalpifolia Gong Tong are used as a Chinese folk herbal medicine for the treatment of enteritis, tonsillitis, bronchitis, and dysentery, etc. Our previous study has identified new C-geranylated flavanones with obvious anti-proliferative effects in lung cancer A549 cells. In the present study, a new C-geranylated flavone, paucatalinone C (1) and five known C-geranylated flavanones (2-6) were isolated.

[Chemical Constituents of Paulownia tomentosa Fruits].

Objective: To investigate the chemical constituents from the fruits of Paulownia tomentosa.

Methods: The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analyses.

Results: Seven compounds were isolated and their structures were identified as ursolic acid (1), sesamin(2),2α,3α, 19a-trihydroxyurs-12-en-28-oic acid(3), luteolin(4), tricin-7-O-β-D-glucoside(5),3',4',5,7-tetrahydroxy-6-[7-hydroxy-3,7-dimethyl-2(E)-octenyl] flavanone(6) and stigmasterol(7).

New geranylated flavanones from the fruits of Paulownia catalpifolia Gong Tong with their anti-proliferative activity on lung cancer cells A549.

Three new geranylated flavanones, named as paucatalinone A (1), B (2), and isopaucatalinone B (3), were isolated from the fruits of Paulownia catalpifolia Gong Tong (Scrophulariaceae). Their structures were well determined by means of IR, MS, 1D and 2D NMR, and CD techniques. Paucatalinone A (1) is the first sample as a dimeric geranylated flavanone derivative isolated from natural products.

[Expression of TM4SF9 in human trophoblasts].

Objective: To investigate the expression of TM4SF9 in the villi of early pregnancy, hydatidiform mole, invasive hydatidiform mole and chorionic carcinoma tissue.

Methods: Immunohistochemistry was used to detect the expression of TM4SF9 in normal villi of early pregnancy, hydatidiform mole, invasive hydatidiform mole and chorionic carcinoma tissues.

Results: TM4SF9 was expressed in the cytotroblasts but not in the syncytiotrophoblast of normal villi.