Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
September 2004
Objective: To develop a method for detection of coxsackie B virus type 1-6 by RT-PCR.
Methods: A pair of primers were designed to amplify all types of coxsackie B virus 1-6 efficiently. The PCR product was hybridized in micro-wells in which 6 type specific oligonucleotide probes had been coated respectively, colorimetric detection was performed to discriminate the types of coxsackie B virus.