Deep metagenomic characterization of the gut virome in pregnant women with preeclampsia.

Preeclampsia (PE), a pregnancy-specific syndrome, has been associated with the gut bacteriome. Here, to investigate the impact of the gut virome on the development of PE, we identified over 8,000 nonredundant viruses from the fecal metagenomes of 40 early-onset PE and 37 healthy pregnant women and profiled their abundances. Comparison and correlation analysis showed that PE-enriched viruses frequently connected to species enriched in PE.

Deep metagenomic characterization of gut microbial community and function in preeclampsia.

Preeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on the fecal samples from 40 early-onset PE and 37 healthy pregnant women.

[Preimplantation Genetic Diagnosis of α/β Complex Thalassemia by Next Generation Sequencing].

Objective: To explore the application value of next generation sequencing (NGS) in preimplantation genetic diagnosis of α/β complex thalassemia couple.

Methods: The coding regions of α-globin genes (HBA1, HBA2) and β-globin gene (HBB) were selected as the target regions. The high-density and closely linked single nucleotide polymorphism (SNP) sites were selected as the genetic linkage markers in the upstream and downstream 2M regions of the gene.

Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women.

Imbalances in gut microbiota composition are linked to hypertension, host metabolic abnormalities, systemic inflammation, and other conditions. In the present study, we examined the changes of gut microbiota in women with early-onset preeclampsia (PE) and in normotensive, uncomplicated pregnant women during late pregnancy and at 1 and 6 weeks postpartum. Gut microbiota profiles of women with PE and healthy pregnant women in the third trimester and at 1 and 6 weeks postpartum were assessed by 16S rRNA gene amplicon sequencing.

[Value of Analyzing Hemoglobin A by ROC Curve for Screening Thalassemia].

Objective: To investigate the value of hemoglobin A(HbA) for screening thalassemia.

Methods: A total of 2 000 adults' peripheral blood samples from Guangdong Women and Children Hospital from June 2013 to January 2014 were collected. The hemoglobin A (HbA) level was analyzed by the full automatic capillary electrophoresis technique, and the genotypes of thalassemia were detected.

[Prokaryotic expression of the extracellular region of human CD1d and preparation of its polyclonal antibody].

Aim: To explore the prokaryotic expression of the extracellular region of human CD1d (hCD1d) and prepare its polyclonal antibody.

Methods: The gene encoding the extracellular region of hCD1d was amplified by PCR and cloned into prokaryotic expression vector pET28, then expressed in E.coli BL21 (DE3) with IPTG induction.

[Preparation and characterization of the rabbit polyclonal antibody against the alpha3 domain of human CD1d].

Aim: To prepare the rabbit antibody against the alpha3 domain of the human CD1d (hCD1d-alpha3).

Methods: The gene fragment coding for hCD1d-alpha3 was amplified by PCR and cloned into prokaryotic expression vector pET28, then expressed in E.coli BL21(DE3).

[Prokaryotic expression of human mast cell chymase gene and preparation of its polyclonal antibody].

Aim: To express the human mast cell chymase cDNA in E.coli and prepare the antibody against human mast cell chymase with recombinant chymase.

Methods: The human mast cell chymase cDNA was cloned by RT-PCR.

[Evaluation on effects of chromogenic medium in rapid detection of Coliform and Escherichia coli].

Objective: To evaluate the detective efficacy of Chromogenic Coliform and Escherichia Coli Agar (CCEA).

Methods: A new chromogenic medium CCEA prepared by Huankai laboratory was used to compare with a classical medium of violet red bile agar (VRBA), and other two Chromogenic media Agar I and Agar II by detecting separately 11 reference strains, thirteen sterile samples with Coliform or E.coli and other four samples, and the accordant rates of detection were observed.

[Preparation and characterization of rabbit antibody against human mast cell carboxypeptidase].

Aim: To prepare antibody against human mast cell carboxypeptidase (hMC-CP) by using recombinant hMC-CP expressed in E.coli, and to characterize the antibody.

Methods: hMC-CP was expressed in E.