(+)-rumphiin and polyalthurea, new compounds from the stems of Polyalthia rumphii.

Two new compounds, (+)-rumphiin (3) and polyalthurea (7), together with seven known ones, 3,4,5-trimethoxy benzoic acid (1), (-)-seselinone (2), cannabisin D (4), allantoin (5), oxostephanine (6) and a mixture of beta-sitosterol (8) and stigmasterol (9) were isolated from the stems of Polyalthia rumphii. The chemical structures of 3 and 7 were elucidated by the combination of spectroscopic data, and the absolute configuration of 3 at C-2 was determined by the matrix method to be R. All compounds were evaluated for their cytotoxicity on four human cancer cell lines, which demonstrated that 3 was a moderate bioactive lignan, and 6 showed significant anticancer activity against SPC-A-1 and BEL-7402 cell lines with IC50 values of 1.

Three new metabolites from Botrytis cinerea.

Three new metabolites, gamma-abscisolactone (1), botrytisic acids A (3) and B (4) were isolated from the fermentation broth of Botrytis cinerea TB-3-H8. Their structures were elucidated on the basis of MS, IR, UV, and NMR spectroscopic data. Compound 2 was isolated from natural resource for the first time.

[Study on the chemical constituents of liposoluble steroidal and triterpenoid compounds from the stem and bark of Macaranga hemsleyana].

Objective: to research the chemical constituents of the liposoluble compounds from the stem and bark of Macaranga hemsleyana Pax & Hoffm.

Methods: Five compounds were obtained by silica chromatography and preparative TLC from the stem and bark of Macaranga hemsleyana Pax & Hoffm., and the chemical structures of these compounds were identified by modern spectroscopy as well as physico-chemical analysis.

[Study on process of removing impurity from water-extraction of gutianquan-capsule].

Objective: To study the result of removing impurity from water-extraction of Gutianquan-capsule with macro-reticular absorbing resin, chitosans clarification, water-extraction and alcohol-precipitation methods.

Method: Coefficient of Unguent of macro-reticular absorbing resin, chitosans clarification method, water-extraction and alcohol-precipitation methods were compared, and qualitative assay of Ginsenoside Rg1, Re, and snide measurement of Ginsenoside Rg1, Ferulic acid, and stability experiment were made.

Result: Coefficient of Unguent of water-extraction and alcohol-precipitation method was 17.

[Pharmacokinetics of the alkaloids from the processed seeds of Strychnos nux-vomica in rats].

Aim: To study the pharmacokinetic process about the concentration in rat plasma of the alkaloids from processed seeds of Strychnos nux-vomica with RP-HPLC method.

Methods: Hypersil BDS C18 column was used and the mobile phase consisted of acetonitrile-water at the flow rate of 0.8 mL.

Analgesic and anti-inflammatory properties of brucine and brucine N-oxide extracted from seeds of Strychnos nux-vomica.

To further understand the purpose of the traditional processing method of the seeds of Strychnos nux-vomica L. (Loganiaceae) as well as analgesic and anti-inflammatory activities of brucine and brucine N-oxide extracted from this medicinal plant, various pain and inflammatory models were employed in the present study to investigate their pharmacological profiles. Both brucine and brucine N-oxide revealed significant protective effects against thermic and chemical stimuli in hot-plate test and writhing test.