Down-regulation of poly (ADP-ribose) polymerase 1 leads to change of hydroquinone cytotoxicity in TK6 cells.

Hydroquinone (HQ), one of the most important metabolites derived from benzene, is known to be associated with acute myelogenous leukemia risk; however, its carcinogenic mechanism remains unclear. In a previous study, we found that low-level of benzene exposure down-regulated the expression of poly(ADP-ribose)polymerase 1 (PARP1). Here, we employed RNA interference to knock down PARP1 expression in TK6 cells and explored the potential role of PARP1 in HQ-induced cytotoxicity.

[Detecting the isoflurane in the air of workplaces with chromatographic method].

Objective: To establish a solvent desorption Gas chromatographic method for detecting the isoflurane in air of workplaces.

Methods: This method is based on "Standardization of methods for determination of toxic substances in workplace air".

Results: This method presents the linear relation with the minimum detectable limit 1.

[Simultaneous determination of seven chemicals of halogenated alkanes and aromatic hydrocarbons in the air of workplace by gas chromatography].

Objective: To establish a gas chromatographic method for determination of halogenated alkanes and aromatic hydrocarbons including trichloromethane, 1,2-dichloroethane, carbon tetrachloride, benzene, toluene, ethylbenzene and xylene in the air of workplace.

Methods: After the air samples collected with activated carbon tubes and desorbed with CS(2), the target toxicants were separated with FFAP capillary columns and detected with flame ionization detector.

Results: The coefficient of correlation was above 0.