Candidatus Liberibacter asiaticus influences the emergence of the Asian citrus psyllid Diaphorina citri by regulating key cuticular proteins.

The Asian citrus psyllid, Diaphorina citri, is the primary vector of the HLB pathogen, Candidatus Liberibacter asiaticus (CLas). The acquisition of CLas shortens the developmental period of nymphs, accelerating the emergence into adulthood and thereby facilitating the spread of CLas. Cuticular proteins (CPs) are involved in insect emergence.

Overexpression of an Integument Esterase Gene Infers the Malathion Detoxification in (Psocoptera: Liposcelididae).

, commonly known as booklouse, is an important stored-product pest worldwide. Studies have demonstrated that booklices have developed resistance to several insecticides. In this study, an integument esterase gene, , with upregulated expression, was characterized in .

Life Table Construction under Different Temperatures and Insecticide Susceptibility Analysis of (Hemiptera: Aphididae).

is an important aphid pest of lettuce, but basic information on its biology is scarce. In this study, effects of three constant temperatures (17, 21, and 25 °C, simulating the mean temperature range in greenhouses) on the development and fecundity of were analyzed by constructing a life table. could develop and reproduce under all three temperatures, but the survival rate, development, and fecundity of were affected by temperature.

CYP4G100 contributes to desiccation resistance by mediating cuticular hydrocarbon synthesis in Bactrocera dorsalis.

The oriental fruit fly Bactrocera dorsalis (Hendel) is expanding its distribution to higher latitudes. Our goal in this study was to understand how B. dorsalis adapts to higher latitude environments that are more arid than tropical regions.

Genome-wide identification of long non-coding RNAs (lncRNAs) associated with malathion resistance in Bactrocera dorsalis.

Background: Long non-coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified in many species including insects. However, the association between lncRNAs and pesticide resistance in insect species such as Bactrocera dorsalis is unknown.

Results: RNA-seq was performed on malathion resistant (MR1) and susceptible (MS) strains of B.

Antioxidant Enzymes and Heat Shock Protein Genes from Are Involved in Stress Defense upon Heat Shock.

Psocids are a new risk for global food security and safety because they are significant worldwide pests of stored products. Among these psocids, has developed high levels of resistance or tolerance to heat treatment in grain storage systems, and thus has led to investigation of molecular mechanisms underlying heat tolerance in this pest. In this study, the time-related effects of thermal stress treatments at relatively high temperatures on the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidases (POD), glutathione-S-transferases (GST) and malondialdehyde (MDA), of were determined.

A Transcriptomic and Proteomic Analysis of the Salivary Glands Reveals Genes Responding to Liberibacter asiaticus.

The Asian citrus psyllid (ACP), Kuwayama, is the principal vector of the Liberibacter asiaticus (CLas) bacterium that causes Huanglongbing (HLB) disease. The salivary glands (SG) is an important barrier to the transmission of CLas. Despite its importance, the transcriptome and proteome of SG defense against CLas are unstudied in .

NADPH-cytochrome P450 reductase mediates the susceptibility of Asian citrus psyllid Diaphorina citri to imidacloprid and thiamethoxam.

Background: The Asian citrus psyllid Diaphorina citri has developed high levels of resistance to many insecticides, and understanding its resistance mechanism will aid in the chemical control of this species. Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is crucial in cytochrome P450 function, and in some insects CPR knockdown has increased their susceptibility to insecticides. However, the CPR from D.

Genome-wide and expression-profiling analyses of the cytochrome P450 genes in Bactrocera dorsalis (Hendel) and screening of candidate P450 genes associated with malathion resistance.

Background: Bactrocera dorsalis (Hendel) is a notorious agricultural pest worldwide, and its resistance to insecticides is a major obstacle in successful control. Cytochrome P450s (P450s) are major metabolic enzymes associated with insecticide resistance. The genome of B.

First Insights into the Intrapuparial Development of (Hendel): Application in Predicting Emergence Time for Tephritid Fly Control.

Intrapuparial development is a special pattern of metamorphosis in cyclorrhaphous flies, in which the pupa forms in an opaque, barrel-like puparium. This has been well studied in forensic insects for age estimations. In this study, the intrapuparial development of a quarantine agricultural pest, (Hendel), was studied under a constant temperature of 27 ± 1 °C and 70 ± 5% relative humidity.

Two delta class glutathione S-transferases involved in the detoxification of malathion in Bactrocera dorsalis (Hendel).

Background: The oriental fruit fly Bactrocera dorsalis (Hendel), a widespread agricultural pest, has evolved resistance to many insecticides, including organophosphorus compounds. Glutathione S-transferases (GSTs) are involved in xenobiotic detoxification and insecticide resistance in many insects. However, the role of delta class GSTs in detoxifying malathion in B.

NADPH-Cytochrome P450 Reductase Mediates the Resistance of () (Kirkaldy) to Abamectin.

NADPH-cytochrome P450 reductase (CPR) plays an essential role in the cytochrome P450 enzyme system, which aids in the metabolism of endogenous and exogenous compounds including the detoxification of insecticides. In this study, the transcript in () (Kirkaldy) was cloned, and the deduced amino acid sequence contained an N-terminal membrane anchor, three conserved binding domains (flavin mononucleotide, flavin adeninedinucleotide, and nicotinamide adenine dinucleotide phosphate), a flavin adeninedinucleotide-binding motif, and catalytic residues. Based on phylogenetic analysis, was grouped in the hemipteran branch.

Identification and expression profiles of fifteen delta-class glutathione S-transferase genes from a stored-product pest, Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae).

Glutathione S-transferases (GSTs) comprise a diverse family of enzymes found ubiquitously in aerobic organisms and they play important roles in insecticide resistance. In this study, we tested the sensitivities of Liposcelis entomophila, collected from four different field populations, to three insecticides. The results showed that the insects from Tongliang population had a relatively higher tolerance to malathion and propuxor than insects from other field populations.