Enhanced treatment of low C/N ratio rural sewage by a modified multi-stage tidal flow constructed wetland at low temperature: Quantitative contributions of key functional genera.

Rural sewage treatment was traditionally faced contradiction between low-treatment rates and the need for low-cost development. To address this challenge, we explored the coupling of effluent circulation and step-feeding strategies in a multi-stage tidal flow constructed wetland (TFCW) to achieve stable nitrogen (N) removal performance under conditions of low carbon-to-nitrogen (C/N) ratios and low temperatures. The modified multi-stage TFCW demonstrated the ability to significantly reduce the concentrations of effluent NH-N and NO-N by 33.