[Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney lines].

Objective: To investigate the expression and function of PKD1 and PKD2 in different kidney tissues and cell lines.

Methods: Immunoprecipitation, Western blotting, In situ hybridization and immunohistochemical staining methods were used to observe the expression of PKD1 mRNA and PKD2 mRNA and their protein abundance in different kidney tissues and cell lines.

Results: Coordinate expressions of PKD1 and PKD2 were found in all kidney tissues and cell lines.

[Gene diagnosis of autosomal dominant polycystic kidney disease type 2 using microsatellite DNA tightly linked to polycystic kidney disease gene 2].

Objective: To use microsatellite DNA tightly linked to polycystic kidney disease gene 2 in the gene diagnosis of autosomal dominant polycystic kidney disease type 2.

Methods: Microsatellite DNA of D4S1534, D4S1542, D4S1563,D4S2460 and D4S423 were amplified with PCR and the fragments of products were analyzed by capillary electrophoresis and Genescan and Genotyper software, and then gene diagnosis of the pedigrees was made by linkage analysis.

Results: Three families were found to be linked to PKD2 in 20 families.

[Mutation detection of PKD2 gene in Chinese by denaturing high-performance liquid chromatograph].

Objective: To detect the mutations of autosomal dominant polycystic kidney disease gene 2(PKD2)in Chinese.

Methods: The white blood cell genomic DNA from patients of 94 Chinese autosomal dominant polycystic kidney disease(ADPKD) pedigrees was isolated and amplified by polymerase chain reaction(PCR). The PCR products were analyzed by denaturing high-performance liquid chromatography(DHPLC).

[Preparation and characterization of monoclonal antibody against N-terminal domain of polycystin 1].

Aim: To prepare and identify monoclonal antibody (mAb) against N-terminal domain of polycystin 1.

Methods: Total RNA was extracted from kidney tissue of a healthy man. Gene sequence encoding polycystin 1 N-terminal domain was amplified by one-step RT-PCR.