A new diagnostic technique for identifying Angiostrongylus spp. larvae in intermediate snail species by examining the buccal cavity.

Background: Angiostrongyliasis is a zoonotic parasitic disease caused by the rat lungworm Angiostrongylus cantonensis. The intermediate hosts of A. cantonensis are gastropods, and snail species such as Pomacea canaliculata play a key role in the transmission of human angiostrongyliasis.

Identification and coregulation pattern analysis of long noncoding RNAs in the mouse brain after Angiostrongylus cantonensis infection.

Background: Angiostrongyliasis is a highly dangerous infectious disease. Angiostrongylus cantonensis larvae migrate to the mouse brain and cause symptoms, such as brain swelling and bleeding. Noncoding RNAs (ncRNAs) are novel targets for the control of parasitic infections.

Identification of hub genes in calcific aortic valve disease.

Calcific aortic valve disease (CAVD) is a heart valve disorder characterized primarily by calcification of the aortic valve, resulting in stiffness and dysfunction of the valve. CAVD is prevalent among aging populations and is linked to factors such as hypertension, dyslipidemia, tobacco use, and genetic predisposition, and can result in becoming a growing economic and health burden. Once aortic valve calcification occurs, it will inevitably progress to aortic stenosis.

Exploration of animal models to study the life cycle of .

The parasite is an important zoonotic parasite. The development of an animal model of 's life cycle is critical for studying the biological characteristics of the parasite in snails and mammals. Eggs of of bovine origin were cultured, and metacercariae were obtained after infection of snails.

MicroRNA-140 inhibits skeletal muscle glycolysis and atrophy in endotoxin-induced sepsis in mice via the WNT signaling pathway.

Sepsis is a systemic inflammatory response syndrome resulting from infection. This study aimed at exploring the role of microRNA-140 (miR-140) in septic mice. Wnt family member 11 (WNT11) was verified to be a target gene of miR-140 after bioinformatic prediction and dual luciferase reporter gene assay.

[Formation and Characteristics of Fasciola hepatica Metacercariae].

The Fasciola hepatica miracidia were used to infect 10 Galba pervia in a random manner. Beginning from day 44 after infection on which 10 metacercariae were found and a total of 495 metacercariae found in 24 days. No signs of cercaria escape or metacercaria formation in the early morning observed during 8 : 00-24 : 00, regardless of the environmental change.

Sphingosine kinase 1 as an anticancer therapeutic target.

The development of chemotherapeutic resistance is a major challenge in oncology. Elevated sphingosine kinase 1 (SK1) levels is predictive of a poor prognosis, and SK1 overexpression may confer resistance to chemotherapeutics. The SK/sphingosine-1-phosphate (S1P)/sphingosine-1-phosphate receptor (S1PR) signaling pathway has been implicated in the progression of various cancers and in chemotherapeutic drug resistance.

[Experimental infection of Galba pervia, Radix swinhoei and Physa acuta with Fasciola hepatica in Dali, Yunnan].

Objective: To determine the intermediate host of Fasciola hepatica in Dali of Yunnan Province, and investigate its development and characteristics.

Methods: F. hepatica eggs from cattle were collected from July 2012 to July 2013, and placed in 28 degrees C water bath for incubation.

[Survey of the number of eggs in Taenia asiatica gravid proglottids in Dali Prefecture of Yunnan Province].

Objective: To observe the number of eggs in the gravid proglottids of Taenia asiatica.

Methods: Twenty gravid proglottids at each end of two adults of T. asiatica were digested by 1% pepsin.

[Molluscicidal effect of WPN and MNSC on Lymnaea].

Objective: To evaluate the molluscicidal effect of wettable powder of 50% niclosamide ethanolamide salt (WPN) and suspension concentrate of 26% metaldehyde and niclosamide (MNSC) on Lymnaea.

Methods: WPN and MNSC were prepared as a series of solutions containing the active ingredient concentrations of 0.06, 0.