[Effects of TRAM-34 on Proliferation and Invasion of Leukemia Cell Line HL-60].

Objective: To investigate the effects of KCa3.1 channel inhibitor TRAM-34 on the proliferation and invasion of leukemia cell line HL-60.

Methods: HL-60 cells at logarithmic growth phase exposed to TRAM-34 at the final concentration of 25, 50, 75 and 100 nmol/L were used as experimental group.

[Effects of nitrogen fertilization on the nitrogen uptake, accumulation, and seed quality of oil peony].

Field experiments, including four levels of N application 0, 18, 24, 30 g N·m, were carried out to clarify the effects of nitrogen fertilization on N accumulation and translocation in lea-ves as well as the seed quality of oil peony (Paeonia ostii 'Fengdan Group'). The results showed that the nitrogen application significantly increased the height, canopy, flower diameter and flower dry mass. The heights under the treatments 24 and 30 g N·m increased by 14.

[The effect of the period of cryopreservative storage on the cryosurvival of human spermatozoa].

Objectives: To study the effect of cryopreservative period on the cryosurvival of human spermatozoa and find out the optimal recovery time of cryopreservation.

Methods: Eighty-eight semen samples were collected from normal donors and divided randomly into 5 groups according to the period of cryopreservative storage (1 d, 7 d, 30 d, 180 d, 300 d) in liquid nitrogen after being frozen by the programized, three-step freezing method. Fresh and frozen-thawed semen were examined by the routine analysis of semen and then the sperm recovery rate were calculated.