This study aimed to explore the relationship between land use landscape pattern and water quality in the upstream of the Gansu water conservation, water and soil erosion, and ecological fragile areas. Based on the land use data and water quality monitoring section in 2020 in the 200 m, 500 m, 1 km, 2 km, 50 km, and 10 km riparian buffer area, the single-factor index evaluation method, random forest regression model, and BP neural network were used to quantify the response relationship between land use and landscape pattern of the upper Yellow River in Gansu province and water quality index and to carry out the basin water quality prediction based on land use landscape index data. The results showed that: ① through the single-factor index method, the major indicators of the total nitrogen (TN) in July and September, dissolved oxygen (DO), permanganate index, ammonia nitrogen (NH -N), total phosphorus (TP), and other surface indexes met the surface water environment class Ⅲ water quality standard.
View Article and Find Full Text PDFNanomaterials (Basel)
August 2024
Anisotropic bulk magnets of ThMn-type SmFeV with a high coercivity () were successfully fabricated. Powders with varying particle sizes were prepared using the ball milling process, where the particle size was controlled with milling time. A decrease in occurred in the heat-treated bulk pressed from large-sized powders, while heavy oxidation excessively occurred in small powders, leading to the decomposition of the SmFeV (1-12) phase.
View Article and Find Full Text PDFZhongguo Shi Yan Xue Ye Xue Za Zhi
October 2018
Objective: To investigate the oxidative stress status and its effects on hepcidin in patients with hemoglobin H Constant Spring disease (HbH-CS).
Methods: A total of 35 patients were enrolled in the study, including 15 splenectomized cases and 20 non-splenectomized cases. 20 healthy volunteers were selected as controls.
Hepatitis B virus (HBV) is a major cause of chronic liver disease, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. HBV polymerase (Pol) is an essential viral protein that is important for HBV replication and might be involved in the development of hepatocellular carcinoma. Protein-protein interactions appears to be crucial for its role.
View Article and Find Full Text PDFZhongguo Dang Dai Er Ke Za Zhi
January 2015
Objectrive: To compare the differences in risk factors for low birth weight (LBW) between Han and Uygur full-term infants and to provide a basis for the prevention of LBW in newborn infants.
Methods: Eighty-seven full-term LBW infants (38 Hans and 49 Uygurs) between March 2013 and June 2014 were selected as the case group, and 186 full-term normal birth weight infants (92 Hans and 94 Uygurs) were selected as the control group. A questionnaire survey was performed to investigate the related factors for LBW.
A total of 10-20% of the population remains unresponsive or weakly responsive to hepatitis B vaccine, which is composed of hepatitis B surface antigen HBsAg (S protein). Therefore, it is necessary to develop a hepatitis B vaccine with a better penetrating and responsive rate. In the present study, a plasmid pVAX1-L-GM was constructed and its immunomodulatory effect of as hepatitis B virus (HBV) DNA vaccine was analyzed through the immunization of BALB/c mice.
View Article and Find Full Text PDFPulmonary hypertension (PHT) is a common complication for patients with β thalassemia intermediate (TI), especially splenectomized patients. However, the frequency and risk factors of PHT in patients with hemoglobin H (HbH) disease is unknown. The purpose of this study was to identify the prevalence of PHT risk manifested as tricuspid regurgitant jet velocity (TRV) ≥2.
View Article and Find Full Text PDFIn order to identify a novel transcript of BRPF1 (BRPF2), a clone separated from mouse cDNA library was sequenced and submitted to GenBank. The expressions of BRPF1 and BRPF2 in different mice tissues were detected using RT-PCR and Northern blotting assays. The preliminary protein functions and conservative domains were analyzed by bioinformatic methods.
View Article and Find Full Text PDFZhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2011
Objective: To explore relevant between human cytomegalovirus (HCMV) infection and college students' neurobehaviors.
Methods: 87 college students were enlisted. They were tested with Bole.
Nan Fang Yi Ke Da Xue Xue Bao
April 2011
Objective: To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase.
Methods: The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol.
Pediatr Blood Cancer
December 2011
Background: β-Thalassemia is extremely prevalent in Guangxi province, Southern China. However, little is known about the treatment and complications of patients with thalassemia major (TM) in Guangxi. The first thalassemia center in China was opened in Guangxi in 2003.
View Article and Find Full Text PDFThe clinical characteristics of 357 patients with hemoglobin H (HbH) disease from the Guangxi province of Southern China were studied. One hundred and ninety-one (53.3%) patients were diagnosed with HbH-Constant Spring, 19 were diagnosed with HbH Westmead.
View Article and Find Full Text PDFParoxysmal nocturnal hemoglobinuria (PNH) is a rare clonal blood disorder that presents chronic intravascular hemolysis. PNH concomitant with inherited hemolytic anemia has been rarely reported. Here, we report an interesting PNH patient who was misdiagnosed with iron deficiency anemia due to concomitant heterozygous β-thalassemia.
View Article and Find Full Text PDFBackground: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that typically causes asymptomatic infections in healthy individuals but may lead to serious complications in newborns and immunodeficient individuals. The emergence of drug-resistant strains of HCMV has posed a need for the development of new drugs and treatment strategies. Antisense molecules are promising gene-targeting agents for specific regulation of gene expression.
View Article and Find Full Text PDFObjective: To observe the after-effect duration of kidney-nourishing and marrow-replenishing therapy on Mediterranean anemia.
Methods: To observe the kidney-nourishing and marrow-replenishing therapy on 58 cases of Mediterranean anemia and the influence of various relative factors on the after-effect duration.
Results: The after-effect duration on 58 cases varied from 3-6 months, about 4 months on average, and was not influenced by sex, clinical types, genetic types, types of Mediterranean anemia and other factors.
pcDNA3.1 in NIH3T3 and Psap-Myc in NIH3T3 cell strains were used as cell models in order to study the effect of prosaposin on cell proliferation, cell apoptosis and its possible molecular mechanism. MTT assay and Annexin V/PI apoptosis kit were used to detect the effect of prosaposin on cell proliferation and cell apoptosis induced by se-rum-starvation stress, respectively.
View Article and Find Full Text PDFZhonghua Yi Xue Yi Chuan Xue Za Zhi
February 2009
Objective: To identify the mutation of the methylmalonic aciduria (cobalamin deficiency) CblC type, with homocystinuria (MMACHC) gene in a pedigree with methylmalonic aciduria.
Methods: The MMACHC gene mutation was detected using polymerase chain reaction (PCR) and DNA sequencing. The MMACHC gene of 50 healthy people was also sequenced as control.
Objective: To research the structure of the unique gene UL148 of the human cytomegalovirus (HCMV) clinical strain in Guangzhou, analyze the relation between its structure polymorphism and HCMV congenital infection, investigate the function of its expression products, and try to reveal the pathogenic mechanism of the gene in HCMV infection in newborns.
Methods: Urine samples were collected from 10 newborns with HCMV infection and inoculated on human fetal lung cells. The viral DNA was isolated, and UL148 gene fragment was amplified and identified.
In order to overcome various malpractices in the traditional teaching methods, and also as part of the Guangdong province molecular biology perfect course project, some reforms were carried out to the teaching pattern of genomics. The reforms include using the foreign original teaching materials, bilingual teaching, as well as taking the constructivism-directed discussion teaching method and the multimedia computer-assisted instruction. To improve the scoring way and the laboratory course of the subject, we carried on a multiplex inspection systems and a self-designing experiments.
View Article and Find Full Text PDFMouse reproductive homeobox on the X chromosome (Rhox) is a novel homeobox gene cluster. Rhox5, also called Pem, belongs to the beta subcluster of Rhox. Codon analysis indicated that the cDNA contains 16% of codons rarely used in Escherichia coli.
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