Changes in the dynamic characteristics of G-protein can alter the immune-protection efficacy of rabies virus vaccine.

Unlabelled: The efficacy of the G-protein is influenced by N-linked glycosylation, which serves as the sole immunogen of the rabies virus vaccine. However, achieving satisfactory immune-protection efficacy remains challenging, owing to the heterogeneous glycosylation of G-proteins. Within molecular dynamics, examining the impact of N-glycan heterogeneity on the structural characteristics of G-proteins provides insights into the relationship between antigens and the efficacy of rabies virus vaccines.

Engineered Living Memory Microspheroid-Based Archival File System for Random Accessible In Vivo DNA Storage.

Given its exceptional durability and high information density, deoxyribonucleic acid (DNA) has the potential to meet the escalating global demand for data storage if it can be stored efficiently and accessed randomly in exabyte-to-yottabyte-scale databases. Here, this work introduces the Engineered Living Memory Microspheroid (ELMM) as a novel material for DNA data storage, retrieval, and management. This work engineers a plasmid library and devises a random access strategy pairing plasmid function with DNA data in a key-value format.

3D-QSAR, design, molecular docking and dynamics simulation studies of novel 6-hydroxybenzothiazole-2-carboxamides as potentially potent and selective monoamine oxidase B inhibitors.

Background: 6-hydroxybenzothiazole-2-carboxamide is a novel, potent and specific inhibitor of monoamine oxidase B (MAO-B), which can be used to study the molecular structure and develop new neuroprotective strategies.

Objective: The aim of this study was to create an effective predictive model from 6-hydroxybenzothiazole-2-carboxamide derivatives to provide a reliable predictive basis for the development of neuroprotective MAO-B inhibitors for the treatment of neurodegenerative diseases.

Methods: First, the compounds were constructed and optimized using ChemDraw and Sybyl-X software.

Development and validation of a deep learning-enhanced prediction model for the likelihood of pulmonary embolism.

Background: Pulmonary embolism (PE) is a common and potentially fatal condition. Timely and accurate risk assessment in patients with acute deep vein thrombosis (DVT) is crucial. This study aims to develop a deep learning-based, precise, and efficient PE risk prediction model (PE-Mind) to overcome the limitations of current clinical tools and provide a more targeted risk evaluation solution.

CircITSN2-miR-17-5p/20a-5p/20b-5p- regulatory network is a potential molecular mechanism of gene involving in immune response to IBDV.

gene is correlated with IBDV immune response in chickens. is a key gene regulating the immune functions of the heart, lung, and proventriculus.CircITSN2-miR-17-5p/20a-5p/20b-5p- is a potential mechanism in IBDV immunity.

From defensive reasoning to innovation: how digital tools foster positive emotions in organizations.

Researchers of organizational learning and innovation have frequently claimed that defensive reasoning is an inducer of negative emotions present in most organizations, inhibiting continuing innovation and development and individuals' effective learning. Although a significant amount of research has been conducted on the factors that reduce defensive reasoning in an organization, the breakthrough process and its outcomes have not yet been studied. This study uses narrative network analysis on longitudinal data from a Chinese tourist resort to explore how organizational factors-such as institutional environments, social media platforms, and virtual-reality role transformations-help employees overcome defensive reasoning and foster innovation.

Diallyl disulfide knee joint injection protects against the early pathologic changes of articular cartilage and subchondral bone in ovariectomized rats.

Background: Osteoarthritis (OA) of the knee joint is a prevalent joint disease that affects most aging individuals worldwide. An extract of garlic oil, diallyl disulfide (DADS), reportedly protects chondrocytes in vitro, with limited data available on its in vivo OA effects. We explored the efficacy of DADS in ovariectomy (OVX)-induced OA rats.

The application of reinforced endotracheal tubes with pressure indicators in preventing postoperative airway-related complications in neurosurgical patients: a randomized controlled study.

Background: Excessive cuff pressure can lead to complications associated with endotracheal intubation. This study aims to compare the effects of cuff inflation guided by a pressure indicator versus the tactile estimation method on postoperative airway-related complications in neurosurgical patients.

Methods: This study employed a prospective, randomized, double-blind, controlled design.

Allograft inflammatory Factor-1 induces the dedifferentiation of Vascular Smooth Muscle cells into a macrophage-like phenotype both in vivo and in vitro.

Atherosclerosis, a chronic lipid-driven vascular inflammatory disease involving multiple cell types, is the primary cause of cardiovascular disease-related morbidity and mortality. Allograft inflammatory factor 1 (AIF-1) contributes to atherosclerosis development by affecting vascular smooth muscle cells (VSMCs). Increasing research indicates that VSMCs are pivotal in atherosclerosis progression, particularly in macrophage-like phenotypic switching, though the mechanism of AIF-1 VSMCs phenotypic switching is not well understood.

Rapid and slow thawing of Takifugu rubripes fillets: TMT-labeled proteomics analysis, biochemical and morphological comparison.

This study aimed to investigate how running water (rapid, R) and ice-water (slow, I) thawing methods affect the quality of Takifugu rubripes fillets. Thawing shrinkage and changes in extractable proteins quantified by tandem mass tag (TMT)-labeled quantitative proteomics were compared. The results showed that the rapidly thawed fillets were quickly underwent greater shrinkage, and the smaller gap areas were reduced by 7.

Development of Self-Aligned Top-Gate Transistor Arrays on Wafer-Scale Two-Dimensional Semiconductor.

Two-dimensional semiconductor materials (2DSM) effectively mitigate the short-channel effect due to their atomic thickness, offering significant advantages over traditional silicon-based materials, particularly in short channel length. In manufacturing 2DSM top-gate field-effect transistors (TG-FETs), simultaneous miniaturization of the gate and channel can only be achieved through a self-alignment process, enabling high-density integration of short-channel FETs. However, current self-aligned FETs based on 2DSM face challenges in attaining wafer-scale integration due to manufacturing process limitations.

1-Deoxynojirimycin Ameliorates Diabetic Liver Injury by Regulating AMPK/SIRT1 and Oxidative Stress in db/db Mice.

Background: Patients with diabetic liver injury are in the dilemma of lowering glucose and protecting liver function. This study aimed to uncover the protective effect and mechanism of 1-deoxynojirimycin (1-DNJ), an alpha-glucosidase inhibitor, against diabetic liver injury.

Methods: The db/db mice were gavaged with 25 mg/kg, 50 mg/kg, and 100 mg/kg of 1-DNJ for 8 weeks.

Variation in and Factors Associated with Youth Self-Harm in College Students - Jiangsu Province, China, 2019-2023.

What Is Already Known About This Topic?: Self-harm represents a significant public health challenge that disproportionately affects young adults. Understanding individual- and institutional-level risk factors is crucial for developing and implementing effective mental wellness intervention programs for college students.

What Is Added By This Report?: The prevalence of youth self-harm fluctuated between 2.

Regulating Integrin β1 to Restore Gonadotropin-Releasing Hormone-Tanycyte Unit Function in Polycystic Ovary Syndrome-Related Hypothalamic Dysregulation.

Excessive gonadotropin-releasing hormone (GnRH) is considered to be an initiating factor in the etiology of polycystic ovary syndrome (PCOS). GnRH neuronal axons terminate at the hypothalamic arcuate nucleus and median eminence, where tanycytes, specialized glial cells, have been proposed to modulate GnRH secretion through plasticity. However, the precise role of the "GnRH-tanycyte unit" during the pathological state of PCOS has not been thoroughly explored.

Pediatric bacterial meningitis in southern China: analysis of 838 cases.

Objective: This work aims to study the clinical features and risk factors of children with bacterial meningitis (BM) in southern China.

Methods: Clinical data of children with BM between 2012 and 2018 from one national center were analyzed retrospectively.

Results: A total of 838 patients (male/female = 1.

Stachydrine targeting tumor-associated macrophages inhibit colorectal cancer liver metastasis by regulating the JAK2/STAT3 pathway.

Introduction: Colorectal cancer (CRC) represents the third most prevalent form of cancer worldwide, with liver metastasis representing a significant contributor to mortality. The interaction between tumor-associated macrophages (TAMs) and tumor cells plays a pivotal role in the development of colorectal cancer liver metastases (CRLM) and represents a promising avenue for therapeutic intervention. Stachydrine (STA), a compound derived from the Leonurus heterophyllus plant, has been shown to effectively inhibit tumor growth through a range of mechanisms.

Activation of zona incerta gamma-aminobutyric acid-ergic neurons alleviates depression-like and anxiety-like behaviors induced by chronic restraint stress.

Background: Depression is a prevalent affective disorder, but its pathophysiology remains unclear. Dysfunction in the gamma-aminobutyric acid (GABA)-ergic system may contribute to its onset. Recently, antidepressants ( brexanolone, zuranolone) targeting the GABA-A receptor were introduced.

Construction and validation of a prognostic signature using WGCNA-identified key genes in osteosarcoma for treatment evaluation.

Background: Osteosarcoma (OS) is an aggressive and fast-growing malignant tumor associated with high mortality. Early diagnosis and prompt treatment can markedly enhance prognosis and increase survival rates. Constructing prognostic models can effectively predict OS progression, assist in patient diagnosis, and provide personalized treatment plans.

Association of memory function with COVID-19 outcomes in adults aged 50 years and older: Analysis of three prospective cohorts.

Background: Patients with Alzheimer's disease or dementia are at increased risk for COVID-19 hospitalization and mortality. However, no study has examined whether memory function is associated with COVID-19 outcomes in general older adults.

Methods: Data were obtained from SHARE (the Survey of Health, Ageing and Retirement in Europe), HRS (the Health and Retirement Study), and ELSA (the English Longitudinal Study of Ageing), three prospective and representative cohorts of non-institutionalized adults aged 50 years and older in 25 European countries plus Israel, the United States, and the United Kingdom, respectively.

Self-Assembled Triple-Targeted Radiosensitizer Enhances Hypoxic Tumor Targeting and Radio-Immunotherapy Efficacy.

Targeted delivery of radiosensitizers and real-time monitoring of hypoxia are crucial for overcoming radiotherapy resistance in hypoxic tumors. Here, we report A-Cy-Ni-RGD, a triple-targeted nitroimidazole (Ni)-linked radiosensitizer that self-assembles into nanoparticles (A-Cy-Ni-RGD NPs) for bimodal near-infrared fluorescence (NIR FL) and photoacoustic (PA) imaging-guided radio-immunotherapy. A-Cy-Ni-RGD NPs specifically accumulate in αβ-positive tumors, where they are hydrolyzed by carboxylesterase to form Cy-Ni-RGD NPs, with enhanced FL at 710 nm and dual PA signals at 680 and 730 nm.

Gαq/11 aggravates acute lung injury in mice by promoting endoplasmic reticulum stress-mediated NETosis.

Background: Acute lung injury (ALI) is distinguished by exaggerated neutrophil extracellular traps (NETs), elevated clinical mortality rates, and a paucity of targeted therapeutic interventions. The Gαq/11 protein, a member of the G protein subfamily, is an effective intervention target for a variety of diseases, but little is known about its role in ALI.

Methods: In this study, a murine model of ALI induced by lipopolysaccharide (LPS) was utilized, employing myeloid cell-specific Gna11 knockout mice.

Bidirectional cohort study protocol to construct and validate a prediction model for perioperative pulmonary complications in elderly hip fracture patients.

Elderly adults with hip fractures are particularly vulnerable to perioperative pulmonary complications (POPCs) throughout the surgical process. While most studies have focused on predicting postoperative pulmonary complications (PPCs), there has been a lack of focus on preoperative and intraoperative phases. To address this gap, this bidirectional cohort study aims to develop and validate a predictive model for POPCs across all surgical stages in elderly patients with hip fracture.

Characterizing Brain-Cardiovascular Aging Using Multiorgan Imaging and Machine Learning.

The structure and function of the brain and cardiovascular system change over the lifespan. In this study, we aim to establish the extent to which age-related changes in these two vital organs are linked. Utilizing normative models and data from the UK Biobank, we estimate biological ages for the brain and heart for 2,904 middle-aged and older healthy adults, including both males and females.

[Predictive value of different obesity indicators for colorectal cancer in different sex populations].

To investigate the predictive value of different obesity indicators for colorectal cancer (CRC) risk in different gender populations. This observational study was conducted within the Kailuan Study (Registration Number: ChiCTR-TNC-11001489). From July 2006 to October 2007, a total of 101,510 employed and retired individuals underwent health examinations, including gastrointestinal disease screening, hematological tests, and questionnaires, at Kailuan General Hospital and its 10 affiliated hospitals.