A novel algorithm for the detection of microsatellite instability in endometrial cancer using next‑generation sequencing data.

The molecular-based detection of microsatellite instability (MSI) in endometrial cancer is complex, due to the low sensitivity of PCR and a lack of standardization in next-generation sequencing (NGS) methods. In the present study, sequenced data were obtained from an NGS panel following the addition of five commonly used microsatellite loci. Subsequently, a novel algorithm, namely MSIPeak, was developed for data analysis.

MiR-21-3p in extracellular vesicles from vascular fibroblasts of spontaneously hypertensive rat promotes proliferation and migration of vascular smooth muscle cells.

Enhanced proliferation and migration of vascular smooth muscle cells (VSMCs) contributes to vascular remodeling in hypertension. Adventitial fibroblasts (AFs)-derived extracellular vesicles (EVs) modulate vascular remodeling in spontaneously hypertensive rat (SHR). This study shows the important roles of EVs-mediated miR-21-3p transfer in VSMC proliferation and migration and underlying mechanisms in SHR.

Effects of EZH2 on Invasion and Migration of Endometrial Stromal Cells in Endometriosis Patients by Regulating PCDH10 Gene H3K27 Methylation.

Context: Endometriosis refers to the appearance of ectopic endometrioid tissue outside the uterus. Low PCDH10 expression has been associated with enhancer of zeste homolog 2 (EZH2), which catalyzes histone 3 (H3K27me3). H3K27me3 is an epigenetic marker associated with endometriosis.

Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells.

Protein tyrosine kinase 6 (PTK6) has shown important cancer‑promoting effects in a variety of cancer types. Nonetheless, its vital role in cervical cancer has not been completely elucidated. The present study sought to address whether PTK6 is involved in the malignant progression of cervical cancer via its interaction with GRB2‑associated binding 1 (GAB1).

The impact of ship emissions on PM and the deposition of nitrogen and sulfur in Yangtze River Delta, China.

Ship emissions contribute significantly to the deterioration of air quality, while their impacts on ambient PM and depositions have not been comprehensively evaluated. This is especially true for China because it has a long coastline, busy shipping routes and many large ports. To fill this gap, this study applied the SMOKE/WRF/CMAQ modeling system to quantifying the impacts of ships on PM compositions, annual and seasonal contribution to PM as well as the wet and dry deposition of nitrogen and sulfur compounds over the land areas in YRD region for 2014.

Mathematical modeling of nitrous oxide production in an anaerobic/oxic/anoxic process.

This study incorporates three currently known nitrous oxide (NO) production pathways: ammonium-oxidizing bacteria (AOB) denitrification, incomplete hydroxylamine (NHOH) oxidation, and heterotrophic denitrification on intracellular polymers, into a mathematical model to describe NO production in an anaerobic/oxic/anoxic (AOA) process for the first time. The developed model was calibrated and validated by four experimental cases, then evaluated by two independent anaerobic/aerobic (AO) studies from literature. The modeling results displayed good agreement with the measured data.