Publications by authors named "Tian Tian Wu"
[Evaluation of the efficacy of 70% ethanol in removing calcium hydroxide in curved root canals].
Shanghai Kou Qiang Yi Xue
October 2024
Purpose: To evaluate the efficacy of micro-computed tomography(Micro-CT) in removing calcium hydroxide from posterior curved root canals.
Methods: Twenty molar teeth (48 root canals) extracted at the Department of General Dentistry, Shanghai Ninth People's Hospital between December 2023 and February 2024 were collected. After preparing by Ni-TI instruments M3 according to standard root canal treatment procedures, calcium hydroxide was injected into the root canals.
Highly Selective Construction of Unique Cyclic [4]Catenanes Induced by Multiple Noncovalent Interactions.
Angew Chem Int Ed Engl
December 2024
The synthesis of high-ordered mechanically interlocked supramolecular structures is an extremely challenging topic. Only two linear [4]catenanes have been reported so far and there is no defined strategy to obtain cyclic [4]catenane. Herein, two unprecedented cyclic [4]catenanes, 1 and 2, were prepared in high yields.
View Article and Find Full Text PDFAs many of the disadvantages of traditional single therapy can be avoided with combination therapy, combination therapy has become a new treatment method. Herein, a long rod-shaped gallium composite multifunctional material (CP-Au-PEG-FA@BSA@IR780) based on chemotherapy therapy (CT), photothermal therapy (PTT) and photodynamic therapy (PDT) is constructed to increase reactive oxygen species (ROS) levels and Au NP release. CP-Au-PEG-FA@BSA@IR780 has fluorescence localization characteristics and can combine with CT-DNA to cause cancer cell apoptosis.
View Article and Find Full Text PDFPurpose: To establish molar root canal model with micro-computed tomography (Micro-CT) and evaluate the removal efficiency of calcium hydroxide by different methods.
Methods: Eight molar teeth (24 root canals) extracted from the Department of General Dentistry, Shanghai Ninth People's Hospital from October 2023 to February 2024 were collected. Root canal preparation was instrumented by M3 according to standard root canal treatment procedures, then calcium hydroxide was injected into the root canal.
Article Synopsis
- Oral chronic graft-versus-host disease (cGVHD) affects the quality of life after hematopoietic stem cell transplantation, and its exact causes are not fully understood, potentially involving microRNA (miRNA) expression and TGF-β/Smad signaling.
- This study investigates the miRNA profiles in the peripheral blood of cGVHD patients, particularly looking at miRNA-769-5p and its relationship with the Smad2 protein.
- Results showed that miRNA-769-5p was expressed differently in patients with and without cGVHD, suggesting it could be a potential diagnostic marker, with Smad2 mRNA showing an inverse relationship with miRNA-769-5p levels.
Article Synopsis
- RNF187 is an E3 enzyme that promotes cancer cell proliferation, but its role in germline cell development was previously unclear.
- Research using a mouse-derived GC-2 cell line showed that RNF187 enhances cell viability, proliferation, and migration by interacting with histone H3.
- The study identifies RNF187's involvement in the ubiquitination of histone H3 at specific lysine residues (K57 and K80), leading to increased gene transcription, suggesting its importance in germ cell development and opening avenues for further research.
Article Synopsis
- - This study highlights the significant role of the long non-coding RNA NRSN2-AS1, which is found to be over-expressed in ovarian cancer tissues and is linked to the progression of the disease in women.
- - NRSN2-AS1 promotes the growth and spread of ovarian cancer cells by stabilizing the protein PTK2 and activating the β-catenin signaling pathway while inhibiting its degradation by MG-53.
- - The findings suggest that targeting the NRSN2-AS1/PTK2/β-catenin signaling axis could have potential clinical applications for treating ovarian cancer, marking a novel discovery in understanding the disease's mechanisms.
Article Synopsis
- Wheat is a vital source of energy and protein for humans, but its production is endangered by fungal diseases like stripe rust.
- The study finds that changes in chloroplast lipid profiles and increased accumulation of jasmonate (JA) in affected areas activate plant defense mechanisms against these pathogens.
- A specific protein, WKS1.1, boosts the production of JA by enhancing the activity of an enzyme involved in its synthesis, leading to improved resistance against the fungal threat.
Article Synopsis
- - A series of silver(I) complexes with O-phenanthroline were created and analyzed using various techniques like IR, MS, NMR, and X-ray crystallography to confirm their structure and properties.
- - The cytotoxic effects of one complex, P-131, were tested on cancer cell lines (HCT-116, HeLa, MDA-MB-231) and a normal cell line (LO2), revealing that P-131 is more effective against cancer cells and safer for normal cells compared to cisplatin, with a lower IC value for HCT-116 (0.86 μM).
- - P-131 was shown to inhibit DNA synthesis, raise reactive oxygen species levels, and demonstrated
Article Synopsis
- - A series of gallium(III) complexes were created and characterized using X-ray crystallography and density functional theory, focusing mainly on their cytotoxic effects against various human cancer cell lines.
- - One specific complex, CP-, showed significant effectiveness in killing colon cancer cells (HCT116) with comparatively lower toxicity than conventional drugs like cisplatin and oxaliplatin.
- - The study revealed that CP- induces apoptosis in cancer cells by affecting DNA-related protein expression and also demonstrated potential for use in colon cancer diagnosis and treatment due to its desirable emissive properties.
Background: Gallium (III) metal-organic complexes have been shown to have the ability to inhibit tumor growth, but the poor water solubility of many of the complexes precludes further application. The use of materials with high biocompatibility as drug delivery carriers for metal-organic complexes to enhance the bioavailability of the drug is a feasible approach.
Methods: Here, we modified the ligands of gallium 8-hydroxyquinolinate complex with good clinical anticancer activity by replacing the 8-hydroxyquinoline ligands with 5-bromo-8-hydroxyquinoline (HBrQ), and the resulting Ga(III) + HBrQ complex had poor water solubility.
Article Synopsis
- Immune dysfunction plays a key role in inflammatory bowel disease (IBD), particularly involving various types of immune cells like B and T lymphocytes, and their imbalance can lead to autoimmune diseases such as IBD.
- The study aimed to determine if curcumin (Cur) could help reduce symptoms of experimental colitis by affecting memory B cells and related signaling pathways.
- The results showed that after 14 days of Cur treatment, mice with colitis experienced improvements in their physical condition and decreased pro-inflammatory cytokines, along with changes in the activation levels of memory B cell subsets.
Establishment of a predictive equation for pulmonary ventilation function in school-aged children in northeast China: a prospective study.
Zhongguo Dang Dai Er Ke Za Zhi
November 2021
Article Synopsis
- The study aimed to create a predictive equation for lung function parameters in children aged 6-16 in northeast China by measuring several airflow and capacity metrics.
- A sample of 504 healthy kids was analyzed using spirometry, focusing on factors like age, height, and weight to establish relationships influencing lung function.
- The findings indicated that boys generally had higher lung capacity measurements than girls in specific age groups, and the new predictive equation developed showed good applicability compared to previous studies.
Curcumin Alleviated Dextran Sulfate Sodium-Induced Colitis by Regulating M1/M2 Macrophage Polarization and TLRs Signaling Pathway.
Evid Based Complement Alternat Med
September 2021
Article Synopsis
- Curcumin shows potential benefits for treating colitis in mice and ulcerative colitis in patients, but its exact mechanism of action related to macrophage polarization is not fully explained.
- In a study, mice with experimentally induced colitis were given curcumin or a standard treatment for 14 days, leading to improvements in weight, colon measurements, and a reduction in colonic damage.
- The treatment with curcumin significantly altered cytokine levels and influenced macrophage types by reducing proinflammatory markers while increasing anti-inflammatory ones, indicating a therapeutic effect through modulation of macrophage polarization and related signaling pathways.
An anion double-walled metal-organic framework [CoLi(BTC)(DMF)(HO)·(CH)N] () based on heterobimetallic Li and Co ions was successfully constructed. Utilizing selective destruction and formation of Co-O/Co-N bonds in the metal chains, [CoLi(BTC)(py)(HO)·(CH)N] () and [CoLi(BTC)(pi)(HO)·(CH)N] () with the same skeleton but distinct pore structures can be surprisingly obtained. Additionally, compounds and can be transformed into [CoLi(BTC)(HO)·(CH)N] () by soaking them in an ethanol solution.
View Article and Find Full Text PDFThe association between intestinal flora and ulcerative colitis (UC) was studied in order to provide a basis and method for clinical treatment. Fresh fecal samples were collected from 30 active UC patients and 10 healthy controls. The intestinal flora DNA from each sample was extracted and 16S rRNA gene sequencing was carried out using HiSeq platform to identify the intestinal flora in fecal samples.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates the role of jasmonic acid (JA) in wheat yield, particularly focusing on the tgw1 mutant, which shows reduced grain weight (GW) and JA levels due to a mutation in the KAT-2B gene involved in JA synthesis.
- - Overexpressing KAT-2B enhances GW in normal wheat and can rescue defects in the tgw1 mutant as well as a related Arabidopsis mutant, indicating its importance in grain development.
- - Interestingly, even though JA synthesis is decreased in the tgw1 mutant, there is an increase in ABA synthesis along with changes in gene expression and chlorophyll content, highlighting a complex regulatory mechanism affecting grain weight.
Article Synopsis
- Injuries to primary teeth can impact a child's appearance, function, and mental health, as well as the development of their permanent teeth, but there is limited evaluation of dentists' knowledge on this topic in China.
- A survey conducted with 394 dentists revealed a 66% correct response rate regarding treatment knowledge for dental trauma, with specific areas like enamel fracture being well understood, while complicated crown-root fractures were less so.
- The study concluded that improving dental trauma education for dentists is crucial, highlighting that factors like specialist training and educational experience positively affect knowledge on the subject.
[Effect of recombinant connective tissue growth factor on proliferation and odontogenic differentiation of dental pulp cells].
Shanghai Kou Qiang Yi Xue
February 2020
Article Synopsis
- The study aimed to investigate how recombinant connective tissue growth factor (rCTGF) affects the growth and development of human dental pulp cells (hDPCs).
- Human dental pulp cells were exposed to varying concentrations of rCTGF, and their growth and mineralization were assessed using assays, with specific emphasis on gene expression related to tooth development.
- Results showed that high levels of rCTGF increased cell proliferation and promoted mineralized nodule formation, suggesting that rCTGF enhances the differentiation of hDPCs by activating the ERK1/2 signaling pathway.
Article Synopsis
- Traumatic dental injuries (TDIs) are a significant global dental health issue, and a study aimed to analyze the trends in TDI research over the last 20 years through a bibliometric approach.
- A total of 2,627 articles were examined, with a noticeable increase in publications, primarily from developed countries, especially Brazil, with "Dental Traumatology" being the leading journal.
- The research focused largely on treatment strategies for TDIs, particularly for permanent teeth, but highlighted a shortage of high-quality studies and a lack of emphasis on prevention and primary dentition.
Article Synopsis
- * Increasing MTF2 levels in HCC cells boosts their growth, migration, and invasion, while reducing its levels has the opposite effect, indicating its role in tumor behavior.
- * MTF2 likely drives these processes by promoting Snail transcription, suggesting that targeting MTF2 could be a new treatment strategy for HCC patients.
SAHH and SAMS form a methyl donor complex with CCoAOMT7 for methylation of phenolic compounds.
Biochem Biophys Res Commun
November 2019
Article Synopsis
- Dietary phenolic compounds, such as anthocyanins and flavonoids, exhibit strong antioxidant and health benefits, with ferulate being a key compound derived from caffeoyl CoA.* -
- The study identifies Arabidopsis CCoAOMT7, which plays a significant role in increasing ferulate levels in the stem cell wall through its interactions with essential proteins in the SAM synthesis cycle.* -
- Disruption of certain proteins, like SAHH1 and SAM synthases, led to a significant decrease in ferulate content, highlighting important connections between protein complexes and cellular methylation processes.*
Article Synopsis
- A meta-analysis was conducted to explore the link between GSTM1 and GSTT1 gene polymorphisms and male infertility specifically among Chinese men.
- The analysis reviewed ten studies focusing on these gene variations, involving thousands of cases and controls, showing that certain null genotypes significantly increase the risk of male infertility.
- Findings suggest that the GSTT1 null genotype is linked to both nonobstructive azoospermia and oligoasthenozoospermia, while the GSTM1 null genotype is only associated with oligoasthenozoospermia, indicating a potential genetic susceptibility in this population.
Novel Role of p53 in Septic Immunosuppression: Involvement in Loss and Dysfunction of CD4+ T Lymphocytes.
Cell Physiol Biochem
December 2018
Article Synopsis
- * The study utilized a mouse model of sepsis to investigate the role of p53 by using both knockout mice and a specific inhibitor (PFT), assessing cell proliferation, apoptosis, and immune function through various assays.
- * Results indicated that elevated p53 levels in CD4+ T lymphocytes led to reduced cell survival and immune dysfunction; inhibiting p53 helped maintain T cell function and improved survival rates in sepsis, undersc
Article Synopsis
- - Sepsis is a major cause of death in intensive care units, characterized by uncontrolled inflammation and immune system failure, leading to poor patient outcomes with limited specific treatments.
- - Autophagy, a cellular survival mechanism, shows promise in preserving immune function and reducing organ failure, which could enhance survival rates in septic conditions.
- - Various drugs and strategies that promote autophagy have been identified as beneficial in sepsis, suggesting that targeting autophagy could help counteract the immune suppression seen in septic patients.