Investigation and study on the epidemiology of gestational diabetes mellitus in Guizhou.

Background: Gestational diabetes mellitus (GDM) is a growing public health concern, particularly in regions with diverse ethnic populations. Understanding the incidence and risk factors of GDM is crucial for early prevention and management, especially in underrepresented areas like Guizhou Province, China, where geographic and ethnic diversity may influence the disease's prevalence and risk profiles.

Aim: To investigate the incidence of GDM and identify its associated risk and protective factors among different ethnic groups in Guizhou Province, providing essential data for early prevention strategies.

Revealing the core suppression effects of various Di (2-ethylhexyl) phthalate exposure on early meiosis progression in postnatal male mice via single-cell RNA sequencing.

The male reproductive system has been the subject of considerable attention in recent years due to the adverse effects of Di (2-ethylhexyl) phthalate (DEHP). Although previous research has suggested that DEHP exposure hinders the early meiotic progression of male germ cells, the underlying mechanisms are still not well understood. The transcriptomic changes in testicular cells of postnatal male rodents following DEHP exposure were meticulously analyzed using 10X Genomics single-cell RNA sequencing in this study.

Association between hyperuricemia and dietary retinol intake in Southwest China: a cross-sectional study based on CHNS database.

Background: Hyperuricemia is increasingly common in Southwestern China and poses significant health risks, including gout and cardiovascular disease. Retinol intake has been hypothesized to affect uric acid levels, but this relationship remains unclear.

Methods: Data from 4,658 participants in the China Health and Nutrition Survey (CHNS) from 1991 to 2018 were analyzed.

Spatiotemporal dynamics of early oogenesis in pigs.

Background: In humans and other mammals, the process of oogenesis initiates asynchronously in specific ovarian regions, leading to the localization of dormant and growing follicles in the cortex and medulla, respectively; however, the current understanding of this process remains insufficient.

Results: Here, we integrate single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) to comprehend spatial-temporal gene expression profiles and explore the spatial organization of ovarian microenvironments during early oogenesis in pigs. Projection of the germ cell clusters at different stages of oogenesis into the spatial atlas unveils a "cortical to medullary (C-M)" distribution of germ cells in the developing porcine ovaries.

Clinical study on the safety and efficacy of remimazolam in hysteroscopic surgery under general anesthesia in elderly patients.

Objective: To evaluate the safety and efficacy of remimazolam in hysteroscopic surgery in elderly patients.

Methods: Following hysteroscopic surgery under selected general anesthesia, 60 elderly patients ASA (American Society of Anesthesiologists) class II-III, >65 years old were randomly assigned to one of two groups: the R group (remimazolam) or the C group (propofol), each with 30 patients. Sufentanil 0.

Evaluating annual soil carbon emissions under biochar-added farmland subjecting from freeze-thaw cycle.

Straw biochar is a commonly recognized agricultural amendment that can improve soil quality and reduce carbon emissions while sequestering soil carbon. However, the mechanisms underlying biochar's effects on annual soil carbon emissions in seasonally frozen soil areas and intrinsic drivers have not been clarified. Here, a 2-y field experiment was conducted to investigate the effects of different biochar dosages (0, 15, and 30, t ha; B0 (CK), B15, and B30, respectively) on carbon emissions (CO and CH) microbial colony count, and soil-environment factors.

Assessing the reproducibility and validity of a food frequency questionnaire for pregnant women from the Chinese Miao ethnic group.

Background: Currently, no food frequency questionnaire is available to be administered exclusively to ethnic minorities in China. This study aimed to evaluate the reproducibility and validity of a culturally tailored semi-quantitative food frequency questionnaire (FFQ) designed for pregnant women belonging to the Miao ethnic group in China.

Methods: A total of 74 questions in the FFQ were administered to collect dietary information from Miao women in China during their pregnancy.

A review of global wilderness area identification since the 21st century.

Wilderness areas are natural landscape elements that are relatively undisrupted by human activity and play a critical role in maintaining ecological equilibrium, preserving naturalness, and ensuring ecosystem resilience. Since 2000, monitoring of global wilderness areas has increased owing to the availability of spatial map data and remote sensing imagery related to human activity and/or human footprint. Progress has been made in the remote sensing of wilderness areas by relying on available historical literature (e.

Tuning the Chiral Growth of Plasmonic Bipyramids via the Wavelength and Polarization of Light.

Circularly polarized light (CPL) is a versatile tool to prepare chiral nanostructures, but the mechanism for inducing enantioselectivity is not well understood. This work shows that the energy and polarization of visible photons can initiate photodeposition at different sites on plasmonic nanocrystals. Here, CPL on achiral gold bipyramids (AuBPs) creates hot holes that oxidatively deposit PbO asymmetrically.

Cognitive Impairment Is Associated with New-Onset Stroke: Evidence from a Nationwide Prospective Cohort Study.

Introduction: Stroke is closely related to cognitive function, and many patients experience cognitive impairment after stroke; however, whether cognitive impairment is associated with an increased risk of stroke remains inconclusive. This study aims to investigate whether cognitive impairment is associated with new-onset stroke (first ever nonfatal stroke) using a national prospective study.

Methods: Data from the China Health and Retirement Longitudinal Study (CHARLS) from 2011 to 2018 were used.

Induced differentiation of primordial germ cell like cells from SOX9 porcine skin derived stem cells.

Understanding the mechanisms behind porcine primordial germ cell like cells (pPGCLCs) development, differentiation, and gametogenesis is crucial in the treatment of infertility. In this study, SOX9 skin derived stem cells (SOX9 SDSCs) were isolated from fetal porcine skin and a high-purity SOX9 SDSCs population was obtained. The SOX9 SDSCs were induced to transdifferentiate into PGCLCs during 8 days of cultured.

Identification of as a candidate marker in Sertoli cell-only syndrome by chromatin immunoprecipitation-sequencing and bioinformatics analysis.

Background: Sertoli cell-only syndrome (SCOS) or germ cell aplasia is one of the most serious histopathological subtypes within the scope of non-obstructive azoospermia (NOA). Understanding the molecular mechanism of SCOS and identifying new non-invasive markers for clinical application is crucial to guide proper sperm procurement and avoid unnecessary interventions. This study sought to identify the differentially expressed genes (DEGs) of SCOS by using gene sequencing identity and verify the key marker genes to provide basic data for subsequent research on SCOS.

Chestnut polysaccharide rescues the damaged spermatogenesis process of asthenozoospermia-model mice by upregulating the level of palmitic acid.

Introduction: In recent years, the quality of male semen has been decreasing, and the number of male infertilities caused by asthenozoospermia is increasing year by year, and the diagnosis and treatment of patients with asthenozoospermia are gradually receiving the attention of the whole society. Due to the unknown etiology and complex pathogenesis, there is no specific treatment for asthenozoospermia. Our previous study found that the administration of chestnut polysaccharide could alter the intestinal microbiota and thus improve the testicular microenvironment, and rescue the impaired spermatogenesis process by enhancing the expression of reproduction-related genes, but its exact metabolome-related repairment mechanism of chestnut polysaccharide is still unclear.

Transcriptomic landscape reveals germline potential of porcine skin-derived multipotent dermal fibroblast progenitors.

According to estimations, approximately about 15% of couples worldwide suffer from infertility, in which individuals with azoospermia or oocyte abnormalities cannot be treated with assisted reproductive technology. The skin-derived stem cells (SDSCs) differentiation into primordial germ cell-like cells (PGCLCs) is one of the major breakthroughs in the field of stem cells intervention for infertility treatment in recent years. However, the cellular origin of SDSCs and their dynamic changes in transcription profile during differentiation into PGCLCs in vitro remain largely undissected.

Nitrogen-Doped Carbon Dot as a Lubricant Additive in Polar and Non-polar Oils for Superior Tribological Properties via Condensation Reaction.

Nitrogen-doped lubricating additives have been proved to be an effective strategy to improve the tribological properties of lubricating oil. However, the traditional preparation methods of nitrogen-doped lubricating additives have the defects including harsh preparation conditions and a time-consuming preparation process. Herein, we report a preparation method of nitrogen-doped carbon dot (NCD) lubricating additives in a short time by one-step aldehyde condensation reaction at room temperature.

Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro.

Background: Many laboratories have described the in vitro isolation of multipotent cells with stem cell properties from the skin of various species termed skin-derived stem cells (SDSCs). However, the cellular origin of these cells and their capability to give rise, among various cell types, to male germ cells, remain largely unexplored.

Methods: SDSCs were isolated from newborn mice skin, and then differentiated into primordial germ cell-like cells (PGCLCs) in vitro.

PINK1-Mediated Mitophagy Promotes Oxidative Phosphorylation and Redox Homeostasis to Induce Drug-Tolerant Persister Cancer Cells.

Unlabelled: The drug-tolerant persister (DTP) state enables cancer cells to evade cytotoxic stress from anticancer therapy. However, the mechanisms governing DTP generation remain poorly understood. Here, we observed that lung adenocarcinoma (LUAD) cells and organoids entered a quiescent DTP state to survive MAPK inhibitor treatment.

Photoemission of the Upconverted Hot Electrons in Mn-Doped CsPbBr Nanocrystals.

Hot electrons play a crucial role in enhancing the efficiency of photon-to-current conversion or photocatalytic reactions. In semiconductor nanocrystals, energetic hot electrons capable of photoemission can be generated via the upconversion process involving the dopant-originated intermediate state, currently known only in Mn-doped cadmium chalcogenide quantum dots. Here, we report that Mn-doped CsPbBr nanocrystals are an excellent platform for generating hot electrons via upconversion that can benefit from various desirable exciton properties and the structural diversity of metal halide perovskites (MHPs).