Complete mitochondrial genome of (Scleractinia, Lobophylliidae): Mitogenome characterization and phylogenetic positioning.

Lack of mitochondrial genome data of Scleractinia is hampering progress across genetic, systematic, phylogenetic, and evolutionary studies concerning this taxon. Therefore, in this study, the complete mitogenome sequence of the stony coral (Ellis & Solander, 1786), has been decoded for the first time by next generation sequencing and genome assembly. The assembled mitogenome is 17,697 bp in length, containing 13 protein coding genes (PCGs), two transfer RNAs and two ribosomal RNAs.

Development and evaluation of a novel in situ target-capture approach for aptamer selection of human noroviruses.

Human noroviruses (HuNoVs) is the primary non-bacterial pathogen causing acute gastroenteritis worldwide. Molecular approaches have been mainly used for detection of HuNoVs. Aptamer-based assay has been also applied for detection of HuNoVs through affinity binding of viral capsid.

Unraveling the Twin and Tunability of the Crystal Domain Sizes in the Medium-Pore Zeolite ZSM-57 by Electron Crystallography.

Tailoring the morphology of a specific crystalline material through distinct crystal growth mechanisms (classical and nonclassical) is challenging. Herein, we report the two unique morphologies of a medium-pore (10×8-ring) zeolite, ZSM-57, prepared by employing an identical organic structure-directing agent (OSDA) and different inorganic cations, namely Na and K , denoted as ZSM-57-Na (pentagonal nanoplates) and ZSM-57-K (pentagonal nanoprisms), respectively. The tunable twin domain size and twin boundaries in both samples have been unraveled at the atomic level by electron crystallography.

[Characteristics of Nitrogen Deposition in Heilongjiang Liangshui National Nature Reserve].

To investigate the nitrogen deposition status in Heilongjiang Liangshui National Nature Reserve, a G78 nitrogen deposition collector was used to monitor dry particulate nitrogen deposition and wet nitrogen deposition during the growing season in 2015, and a bucket was used to monitor bulk nitrogen deposition during non-growing season. The results showed that:①Total nitrogen deposition (wet nitrogen deposition+dry particulate nitrogen deposition during the growing season and bulk deposition during the non-growing season) was 12.93 kg·(hm·a), inorganic nitrogen deposition was 8.

Synthesis of nanosized SAPO-34 with the assistance of bifunctional amine and seeds.

Nanosized SAPO-34 with a tunable silica content (acidity) has been synthesized with the assistance of bifunctional amine 1-[2-(2-hydroxyethoxy)ethyl]piperazine (HEEP) or its analog 1-(2-hydroxyethyl)piperazine (HEP), in which the piperazinyl group acting as a co-template prompts the formation of the pure CHA phase, and the hydroxyl/ether groups help inhibit the crystal growth. The resultant nanocrystallines possess a large amount of Si(4Al) species, improve transport properties as confirmed by IGA, and thus exhibit enhanced MTO catalytic performance.

A Successful Limb Salvage of an Electrical Burned Patient With Extensive Soft Tissue and Femoral Bone Necrosis.

Severe electrical injury presents a formidable therapeutic challenge for surgeons because of the requirement for complex soft tissue and bony reconstruction to restore functionality of the injured area. The authors report on the limb salvage procedure for a patient with extensive soft tissue and femoral bone necrosis from a high-voltage electrical burn. The right thigh and knee were burned, resulting in large areas of muscle necrosis and a long segment of distal femur exposure and necrosis.

Preparation of Spherical Mordenite Zeolite Assemblies with Excellent Catalytic Performance for Dimethyl Ether Carbonylation.

Millimeter-sized spherical mordenite (MOR) zeolite with good mechanical strength was successfully prepared via vapor-assisted transformation of Na- and Al-doped SiO spheres. It is demonstrated that the precursor prepared by vacuum impregnation is essential for the achievement of well-retained morphology; the presence of organic amine (such as TEAOH, TMAOH, and HMI) in the vapor can help promote the crystallization, avoid the core-shell morphology, and improve the zeolite Si/Al ratio, although the MOR crystal morphology in the spherical products varies with the choice of organic amines. The resultant MOR-TEAOH spheres, which were synthesized under the assistance of TEAOH, possess large amount of Brønsted acid sites in the 8-MR side pockets (0.

A New Facile Route to Flexible and Semi-Transparent Electrodes Based on Water Exfoliated Graphene and their Single-Electrode Triboelectric Nanogenerator.

Wearable technologies are driving current research efforts to self-powered electronics, for which novel high-performance materials such as graphene and low-cost fabrication processes are highly sought.The integration of high-quality graphene films obtained from scalable water processing approaches in emerging applications for flexible and wearable electronics is demonstrated. A novel method for the assembly of shear exfoliated graphene in water, comprising a direct transfer process assisted by evaporation of isopropyl alcohol is developed.

Quantitative characterization of turbidity by radiative transfer based reflectance imaging.

A new and noncontact approach of multispectral reflectance imaging has been developed to inversely determine the absorption coefficient of μ , the scattering coefficient of μ and the anisotropy factor g of a turbid target from one measured reflectance image. The incident beam was profiled with a diffuse reflectance standard for deriving both measured and calculated reflectance images. A GPU implemented Monte Carlo code was developed to determine the parameters with a conjugate gradient descent algorithm and the existence of unique solutions was shown.

Role of Teriparatide in Glucocorticoid-induced Osteoporosis through Regulating Cellular Reactive Oxygen Species.

Objective: To determine the signaling pathways mediated by teriparatide in MLO-Y4 cell lines based on the evaluation of reactive oxygen species (ROS) through AKT pathways, which regulate apoptosis of bone cells.

Methods: We performed the DCFH-DA assay to investigate the role of ROS in MLO-Y4 cells caused by dexamethasone (Dex). Four groups were included: Dex group, Dex+NAC, Dex+ teriparatide group and control group (without any dispose).

[Fine root morphology and chemistry characteristics in different branch orders of Castanopsis platyacantha and their responses to nitrogen addition].

Fine root morphology and chemistry characteristics of dominant species Castanopsis platya-cantha in an evergreen broad-leaved forest in subtropical China and their response to nitrogen (N) addition were determined in order 1st to 5th. With the increase of root order, C. platyacantha root diameter, tissue density (RTD), and K content increased, and specific root length (SRL), speci-fic surface area (SRA) and contents of N, P and Mg decreased.

Intralesional bleomycin A5 injection for the treatment of nasal polyps through inducing apoptosis.

Conclusion: An intralesional bleomycin A5 (BLE) injection might be used as an alternative therapy for eosinophilic-type nasal polyps (NPs). BLE-induced apoptosis might play an important role in shrinkage of NPs.

Objectives: The aim of this study is to determine the effect and explore the possible role of apoptosis in shrinkage of NPs.

A wafer-scale 1 nm Ni(OH) nanosheet with superior electrocatalytic activity for the oxygen evolution reaction.

A wafer-scale 1.4 nm ultrathin Ni(OH) nanosheet was synthesized by ionic layer epitaxy. This free-standing Ni(OH) nanosheet was directly used to catalyze the oxygen evolution reaction (OER).

Carbon quality and soil microbial property control the latitudinal pattern in temperature sensitivity of soil microbial respiration across Chinese forest ecosystems.

Understanding the temperature sensitivity (Q ) of soil organic C (SOC) decomposition is critical to quantifying the climate-carbon cycle feedback and predicting the response of ecosystems to climate change. However, the driving factors of the spatial variation in Q at a continental scale are fully unidentified. In this study, we conducted a novel incubation experiment with periodically varying temperature based on the mean annual temperature of the soil origin sites.

Induction of apoptosis in nasal polyp-derived fibroblasts by bleomycin A5 in vitro.

The present study aimed to evaluate the pro-apoptotic effects of bleomycin A5 on nasal polyp‑derived fibroblasts (NPDFs) and the underlying molecular mechanisms. Nasal polyp tissue was acquired from 10 patients during surgery and NPDFs were isolated from surgical tissues. Fibroblasts were identified using immunohistochemistry.

The complete mitochondrial genome of the (Scleractinia: Acroporidae).

In this study, the complete mitogenome sequence of stony coral, (Scleractinia), has been decoded for the first time by next generation sequencing and genome assembly. The assembled mitogenome, consisting of 17,884 bp, has unique 13 protein coding genes (PCGs), three transfer RNAs, and two ribosomal RNAs genes. The complete mitogenome of showing 99% identities to .

A Bacterial Surface Display System Expressing Cleavable Capsid Proteins of Human Norovirus: A Novel System to Discover Candidate Receptors.

Human noroviruses (HuNoVs) are the dominant cause of food-borne outbreaks of acute gastroenteritis. However, fundamental researches on HuNoVs, such as identification of viral receptors have been limited by the currently immature system to culture HuNoVs and the lack of efficient small animal models. Previously, we demonstrated that the recombinant protruding domain (P domain) of HuNoVs capsid proteins were successfully anchored on the surface of BL21 cells after the bacteria were transformed with a plasmid expressing HuNoVs P protein fused with bacterial transmembrane anchor protein.

Forest conversion induces seasonal variation in microbial β-diversity.

World-wide conversion of natural forests to other land uses has profound effects on soil microbial communities. However, how soil microbial β-diversity responds to land-use change and its driving mechanisms remains poorly understood. In this study, therefore, we examined the effect of forest conversion from native broad-leaved forest to coniferous plantation on soil microbial β-diversity and its underlying mechanisms in both summer and winter in subtropical China.

The Effect of Obesity on Clinical Outcomes After Minimally Invasive Surgery of the Spine: A Systematic Review and Meta-Analysis.

Background: Obesity is associated with increasing morbidity and mortality in many prevalent diseases, especially lumbar degenerative disease. The relationship between minimally invasive surgery (MIS) of the spine and perioperative adverse events in obese patients with lumbar degenerative disease has not been well evaluated.

Methods: We conducted a systematic review and meta-analysis to identify relevant studies involving obese patients with spine MIS in electronic databases up to June 2017, including Web of Science, Embase, PubMed, the Cochrane Controlled Trials Register, and the Cochrane Library.

Binding of Does Not Protect Tulane Virus from Heat-Inactivation Regardless the Expression of HBGA-Like Molecules.

Histo-blood group antigens (HBGAs) are considered as receptors/co-receptors for human norovirus (HuNoV). It has been reported that binding of HuNoV-derived virus-like particles (VLPs) to HBGA-like molecules-expressing bacteria increased the stability of VLPs to heat-denaturation (HD). In this study, we tested for HBGA-like-binding-conveyed protection against HD on viral replication using Tulane virus (TV) and O86:H2 (O86:H2), with K-12 (K-12) used as a control.

The complete mitochondrial genome of the .

In this study, the complete mitogenome sequence of stony coral, (Scleractinia), has been decoded for the first time by next generation sequencing and genome assembly. The assembled mitogenome, consisting of 18,480 bp, has unique 13 protein-coding genes (PCGs), three transfer RNAs, and two ribosomal RNAs genes. The complete mitogenome of showing 99% identities to .

Concurrent Detection of Human Norovirus and Bacterial Pathogens in Water Samples from an Agricultural Region in Central California Coast.

Bacterial pathogens and human norovirus (HuNoV) are major cause for acute gastroenteritis caused by contaminated food and water. Public waterways can become contaminated from a variety of sources and flood after heavy rain events, leading to pathogen contamination of produce fields. We initiated a survey of several public watersheds in a major leafy green produce production region of the Central California Coast to determine the prevalence of HuNoV as well as bacterial pathogens.

Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method.

Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV in environmental water samples. This assay was based on capturing encapsidated HuNoV by viral receptors, followed by in situ amplification of the captured viral genomes by RT-qPCR.

Effect of micro strain stress on proliferation of endothelial progenitor cells in vitro by the MAPK-ERK1/2 signaling pathway.

Background: The aim of this study was to analyze the effect of micro strain stress on the proliferation of endothelial progenitor cells in vitro by the MAPK-ERK1/2 signaling pathway.

Material And Methods: In this study, we report the effect of micro strain stress on proliferation of endothelial progenitor cells (EPCs) in vitro by loading different types of micro strain stress on EPCs. We also explore the possible mechanisms of this phenomenon.

Effects of three years of simulated nitrogen deposition on soil nitrogen dynamics and greenhouse gas emissions in a Korean pine plantation of northeast China.

Continuously enhanced nitrogen (N) deposition alters the pattern of N and carbon (C) transformations, and thus influences greenhouse gas emissions. It is necessary to clarify the effect of N deposition on greenhouse gas emissions and soil N dynamics for an accurate assessment of C and N budgets under increasing N deposition. In this study, four simulated N deposition treatments (control [CK: no N addition], low-N [L: 20kgNhayr], medium-N [M: 40kgNhayr], and high-N [H: 80kgNhayr]) were operated from 2014.