High-level production of poly-γ-glutamic acid by a newly isolated sp. YJY-8 and potential use in increasing the production of tomato.

Strain YJY-8, a new γ-polyglutamic acid producer, was separated from fermented soybean paste samples. The strain was identified as a genus of by morphological and 16S rDNA sequence analysis and was named YJY-8. The optimal medium composition and cultural conditions were studied using a single-factor experiment and a response surface experiment.

Optimization of fermentation conditions for production of neutral metalloprotease by SCK6 and its application in goatskin-dehairing.

In this study, a high protease-producing strain was screened by spread plate method and identified by molecular biology and morphological identification. It was identified as sp. LCB14.

sp. nov., isolated from chromium-containing chemical plant soil.

A Gram-stain-positive, rod-shaped, aerobic, non-motile, non-sporulating bacterial strain, designated CSA1, was isolated from chromium-containing soil sampled at a chemical plant. Growth of strain CSA1 occurred at pH 6-10 (optimum, pH 7), 15-45 °C (optimum, 30 °C) and in the presence of 0.5-6.

Cloning, heterologous expression and characterization of a novel streptomyces trypsin in Bacillus subtilis SCK6.

A novel streptomyces trypsin GM2938 was selected as the object of study. The active GM2938 contains 223 amino acid residues. Constructing recombinant plasmid and transforming Bacillus subtilis SCK6, the heterogenous expression of GM2938 was achieved.

Enhanced extracellular recombinant keratinase activity in SCK6 through signal peptide optimization and site-directed mutagenesis.

Keratinase has a great commercial value owing to its applications in the enzymatic dehairing of goatskins. In this study, we adopted a combined strategy to enhance the extracellular recombinant keratinase activity in SCK6. First, nine signal peptides were screened to enhance the expression of extracellular keratinase.

Glycine betaine enhances biodegradation of phenol in high saline environments by the halophilic strain sp. PT-20.

The halophilic bacterial strain PT-20, isolated from saline alkali soil samples and identified as a member of the genus , exhibited a robust ability to degrade phenol under high salt conditions. It was determined that strain PT-20 was capable of degrading 1000 mg L phenol completely in the presence of 10% NaCl within 120 h. Under the optimal degradation conditions, pH 8.

High-expression keratinase by Bacillus subtilis SCK6 for enzymatic dehairing of goatskins.

An extracellular keratinase gene from Bacillus sp. LCB12, isolated from saline-alkali soil, was cloned and high-effectively expressed in Bacillus subtilis SCK6. The enzyme was purified by ammonium sulphate precipitation and cation-exchange chromatography.

Culturable epiphytic bacteria isolated from Teleogryllus occipitalus crickets metabolize insecticides.

The development of insecticide resistance is attributed to evolutionary changes in pest insect genomes, such as alteration of drug target sites, upregulation of degrading enzymes, and enhancement of drug excretion. Beyond these well-known mechanisms, symbiotic bacteria may confer insecticide resistance to host crickets. The current study was designed to screen all possible culturable bacterial groups found living in and on the bodies of Teleogryllus occipitalis crickets.

Adaptations of HNA-14 required for long-term survival under osmotic challenge: a multi-omics perspective.

Genomic sequence, transcriptomic, metabolomic and fatty acid analyses of strain HNA-14 were performed to understand the mechanism of salt tolerance for long-term survival. The results indicated that strain HNA-14 has different osmotic resistance mechanisms for long-term survival and short-term salt stress. The cells mainly synthesized compatible solutes to resist osmotic pressure when cultured under nutrient deficient conditions, while they can slow down the synthesis rate and uptake from the environment when cultured under a nutritionally rich environment.

Streptomyces dioscori sp. nov., a Novel Endophytic Actinobacterium Isolated from Bulbil of Dioscorea bulbifera L.

A novel endophytic actinobacterium strain, A217, was isolated from the bulbil of Dioscorea bulbifera L. Its taxonomic position was characterized using a polyphasic study. Morphological and chemotaxonomic characteristics of strain A217 were consistent with those of members of the genus Streptomyces: long straight to flexuous spore chain; cellular components contained LL-diaminopimelic acid, ribose, and small traces of glucose in whole-cell hydrolysates; MK-9(H) and MK-9(H) as predominant menaquinones.

Actinocorallia populi sp. nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.).

An endophytic actinobacterium, strain A251, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy. Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201 (98.0 %) and Actinocorallia libanotica IFO 10495 (98.

Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil.

A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169, was isolated from a saline soil sample from Gansu Province, PR China. The cells of LCB169 grew at 10-52 °C (optimum 30 °C), at pH 6.0-10.

Ornithinibacillus salinisoli sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.

A taxonomic study was performed on strain LCB256, which was isolated from a saline-alkali soil sample taken from northwestern China. Cells of strain LCB256 were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.

Screening cyhalothrin degradation strains from locust epiphytic bacteria and studying Paracoccus acridae SCU-M53 cyhalothrin degradation process.

All locust epiphytic bacteria were screened and a total of 62 epiphytic bacteria were obtained from samples of Acrida cinerea. Via phylogenetic analysis, the 62 epiphytic bacteria were allocated to 27 genera, 18 families, 13 orders, six classes, and four phylums. Then, cyhalothrin degradation experiments were conducted, and the 10 strains that degraded more than 30% cyhalothrin and Paracoccus acridae SCU-M53 showed the highest cyhalothrin degradation rate of 70.

Planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.

A novel aerobic, Gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated LCB217, was isolated from a saline-alkali soil in north-western China and identified using a polyphasic taxonomic approach. Growth occurred with 3-15 % (w/v) NaCl (optimum 3-5 %), at 10-45 °C (optimum 30 °C) and at pH 7.0-9.

Microbial transformation of resveratrol by endophyte Streptomyces sp. A12 isolated from Polygonum cuspidatum.

Resveratrol (1) undergoes microbial transformation when fermented with Streptomyces sp. A12 to yield 3, 5, 4'-trimethoxy-trans-stilbene (2). The structure of the compound 2 was elucidated using the modern spectroscopic techniques.

Diversity, bioactivities, and metabolic potentials of endophytic actinomycetes isolated from traditional medicinal plants in Sichuan, China.

The present study was designed to determine the taxonomic diversity and metabolic activity of the actinomycetes community, including 13 traditional medicinal plants collected in Sichuan province, China, using multiple approaches such as morphological and molecular identification methods, bioactivity assays, and PCR screening for genes involved in antibiotics biosynthesis. 119 endophytic actinomycetes were recovered; 80 representative strains were chosen for 16S rRNA gene partial sequence analyses, with 66 of them being affiliated to genus Streptomyces and the remaining 14 strains being rare actinomycetes. Antimicrobial tests showed that 12 (15%) of the 80 endophytic actinomycetes displayed inhibitory effects against at least one indicator pathogens, which were all assigned to the genus Streptomyces.

Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.

A moderately halophilic bacterium, strain HNA-14(T), was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province. On the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus Bacillus. The organism grew optimally at 30 °C and pH 8.

Purification and characterization of a cold-adapted lipase from Oceanobacillus strain PT-11.

We isolated a moderately halophilic lipase-producing bacterium from the saline soil. Based on the morphological, physiological, chemotaxonomic and phylogenetic analysis, the isolate PT-11 was postulated to be a novel species identified as Oceanobacillus rekensis PT-11. The lipase was purified 2.