Effective Gene Expression Prediction and Optimization from Protein Sequences.

High soluble protein expression in heterologous hosts is crucial for various research and applications. Despite considerable research on the impact of codon usage on expression levels, the relationship between protein sequence and expression is often overlooked. In this study, a novel connection between protein expression and sequence is uncovered, leading to the development of SRAB (Strength of Relative Amino Acid Bias) based on AEI (Amino Acid Expression Index).

Achieving Photocatalytic Overall Nitrogen Fixation via an Enzymatic Pathway on a Distorted CoP Configuration.

Photocatalytic nitrogen (N) fixation over semiconductors has always suffered from poor conversion efficiency owing to weak N adsorption and the difficulty of N≡N triple bond dissociation. Herein, a Co single-atom catalyst (SAC) model with a C-defect-evoked CoP distorted configuration was fabricated using a selective phosphidation strategy, wherein P-doping and C defects co-regulate the local electronic structure of Co sites. Comprehensive experiments and theoretical calculations revealed that the distorted CoP configuration caused a strong charge redistribution between the Co atoms and adjacent C atoms, minimizing their electronegativity difference.

Corrole-based photothermal nanocomposite hydrogel with nitric oxide release for diabetic wound healing.

The management of chronic diabetic wounds remains a significant challenge due to persistent bacterial infections and impaired angiogenesis. Herein, we reported a nanocomposite hydrogel (M/P-SNO/G) incorporated with M/P-SNO nanoparticles engineered by supramolecular assembly of the photosensitizing mono-carboxyl corrole (MCC) and S-nitrosothiol-modified polyethylene glycol (mPEG-SNO) for synergistic photothermal therapy (PTT)/nitric oxide (NO) treatment of diabetic wounds. The strong π-π interaction among aggregated MCC in M/P-SNO enhances the optical absorption and photothermal ability, thereby facilitating the precise release of NO upon laser irradiation.

Achieving thermostability of a phytase with resistance up to 100 °C.

The development of enzymes with high-temperature resistance up to 100 °C is of significant and practical value in advancing the sustainability of industrial production. Phytase, a crucial enzyme in feed industrial applications, encounters challenges due to its limited heat resistance. Herein, we employed rational design strategies involving the introduction of disulfide bonds, free energy calculation, and B-factor analysis based on the crystal structure of phytase APPAmut4 (1.

On the remarkable resistance to oxidation by the Bi cluster.

The reactivity of Bi clusters ( = 2 to 30) with O is found to display even-odd alternations. The open-shell even-sized Bi clusters are more reactive than the closed-shell odd-sized clusters, except Bi, which exhibits no observable reactivity toward O. We have investigated the structure and bonding of Bi to understand its remarkable resistance to oxidation.

Electric Field and Nanocontact Effects in Metal-Organic Framework/LiLaZrTaO Ionic Conductors for Fast Interfacial Lithium-Ion Transport Kinetics.

The slow ion transport kinetics inside or between the nanofillers in composite polymer electrolytes (CPEs) lead to the formation of lithium dendrites for solid-state lithium batteries. To address the critical issues, CPEs (U@UNL) composed of a UIO-66@UIO-66-NH (U@UN) core-shell heterostructure and LiLaZrTaO (LLZTO) filler is designed. Due to the different band structures of the U@UN heterostructure, a built-in electric field is constructed to promote the transfer kinetics of carriers.

Engineering dual-functional and thermophilic BMHETase for efficient degradation of polyethylene terephthalate.

Polyethylene terephthalate (PET) biodegradation is hindered by the intermediates bis (2-hydroxyethyl) terephthalate (BHET) and mono (2-hydroxyethyl) terephthalate (MHET). BMHETase, a thermophilic hydrolase identified from the UniParc database, exhibits degradation activity towards both BHET and MHET. BMHETase showed higher activity on BHET than LCCICCG and FASTPETase at temperatures ranging from 50 to 70℃.

Unveiling the Influences of In Situ Carbon Content on the Structure and Electrochemical Properties of MoS/C Composites.

In this work, a MoS/C heterostructure was designed and prepared through an in situ composite method. The introduction of carbon during the synthesis process altered the morphology and size of MoS, resulting in a reduction in the size of the flower-like structures. Further, by varying the carbon content, a series of characterization methods were employed to study the structure and electrochemical lithium storage performance of the composites, revealing the effect of carbon content on the morphology, structure characteristics, and electrochemical performance of MoS/C composites.

Dipyridamole-grafted copolymer electrospun nanofiber membranes for suppression of peritendinous adhesions.

Post-traumatic tendon adhesions significantly affect patient prognosis and quality of life, primarily stemming from the absence of effective preventive and curative measures in clinical practice. Current treatment modalities, including surgical excision and non-steroidal anti-inflammatory drugs, frequently exhibit limited efficacy or result in severe side effects. Consequently, the use of anti-adhesive barriers for drug delivery and implantation at the injury site to address peritendinous adhesion (PA) has attracted considerable attention.

The seed endophytic microbe Microbacterium testaceum M15 enhances the cold tolerance and growth of rice (Oryza sativa L.).

The potential of seed endophytic microbes to enhance plant growth and resilience is well recognized, yet their role in alleviating cold stress in rice remains underexplored due to the complexity of these microbial communities. In this study, we investigated the diversity of seed endophytic microbes in two rice varieties, the cold-sensitive CB9 and the cold-tolerant JG117. Our results revealed significant differences in the abundance of Microbacteriaceae, with JG117 exhibiting a higher abundance under both cold stress and room temperature conditions compared to CB9.

Advanced Synthesis of Hierarchically Porous Zeolite Catalysts Utilizing Biomass Templates for the Catalytic Pyrolysis of Stearic Acid into Short-Chain Olefins.

In this study, hierarchically porous ZSM-5 catalysts were fabricated by one-pot assembling ZSM-5 particles onto diverse biomass templates (e.g., rice husk, tea seed husk, tung shell, and coconut shell), wherein the biomass template was transformed into bio-SiO or biochar depending on the calcination conditions.

[Evolution Characteristics of PM and O and Their Synergistic Effects on Atmospheric Compound Pollution in Tangshan].

The concentrations of atmospheric pollutants PM, O, SO, NO, and CO together with the meteorological factors of temperature （）, relative humidity （RH）, wind speed, and other relevant data in Tangshan from 2015 to 2021 were collected to study the variation characteristics of PM and O at different periods in Tangshan City in the past seven years and their influencing factors, to discuss the contributions of air mass transport to PM and O pollution, and to reveal the synergistic influence mechanism of PM and O on atmospheric compound pollution by using correlation analysis and backward trajectory cluster analysis techniques. The results showed that PM concentrations in Tangshan decreased year by year from 2015 to 2021, whereas O concentration showed a unimodal trend, with the peak appearing in 2017. Both PM and O concentrations showed obvious seasonal variation trends； PM was characterized by the highest concentration in winter and the lowest concentration in summer, whereas O was characterized by the highest concentration in summer and the lowest concentration in winter.

Cu-Bi Bimetallic Sulfides Loaded on Two-Dimensional TiCT MXene for Efficient Electrocatalytic Nitrogen Reduction under Ambient Conditions.

In this paper, TiCT MXene/Cu-Bi bimetallic sulfide (TiCT/BiCuS) composites were prepared by a simple deposition method for electrocatalytic nitrogen reduction reaction (eNRR). Compared to TiCT/BiS and TiCT/CuS, the eNRR performance of TiCT/BiCuS is significantly improved. The results show that TiCT/BiCuS exhibits a NH yield of 62.

Optimal Structure and Photocatalytic Performance of CN@TiO Composite by Controlling the Molar Ratio of Components.

Based on the heterogeneous composite design of CN and TiO, composite photocatalysts of CN@TiO (with varied molar ratios of CN to TiO) were synthesized by a water bath method to degrade RhB in wastewater. The composition, morphology, structure, and photocatalytic properties of the materials were assessed through a variety of characterization techniques. The results show that TiO nanoparticles are uniformly coated on two-dimensional g-CN nanosheets, forming relatively dense heterostructures within the CN@TiO composite.

Enhanced Photoemission Performance of InGaN Inclined Nanowire Array.

We propose to improve the solar energy utilization by using InGaN inclined nanowire array photocathodes. We first study vertical nanowire array. On the basis of vertical nanowire array, we study inclined nanowires by changing the inclination angle of nanowires.

Synergistic effect of interstitial phosphorus doping and MoS modification over ZnCdS for efficient photocatalytic H production.

ZnCdS photocatalysts have been widely investigated due to their diverse morphologies, suitable band gaps/band edge positions, and high electronic mobility. However, the sluggish charge separation and severe charge recombination impede the application of ZnCdS for hydrogen evolution reaction (HER). Herein, doping of phosphorus (P) atoms into ZnCdS has been implemented to elevate S vacancies concentration as well as tune its Fermi level to be located near the impurity level of S vacancies, prolonging the lifetime of photogenerated electrons.

Long-Term Outcomes of Sex Differences in Three-Vessel Coronary Disease with Different Treatment Strategies: A Large Cohort Study.

Aim: The information assessing sex differences in outcomes of patients with three-vessel coronary disease (TVD) after different treatment strategies is sparse. This study aimed to investigate long-term outcomes of TVD among women compared with men after medical therapy (MT) alone, percutaneous coronary intervention (PCI), or coronary artery bypass grafting surgery (CABG).

Methods: Consecutive 8943 patients with TVD were enrolled.

Research on Active Safety Situation of Road Passenger Transportation Enterprises: Evaluation, Prediction, and Analysis.

The road passenger transportation enterprise is a complex system, requiring a clear understanding of their active safety situation (ASS), trends, and influencing factors. This facilitates transportation authorities to promptly receive signals and take effective measures. Through exploratory factor analysis and confirmatory factor analysis, we delved into potential factors for evaluating ASS and extracted an ASS index.