Two-stage continuous cultivation of microalgae overexpressing cytochrome P450 improves nitrogen and antibiotics removal from livestock and poultry wastewater.

Improper treatment of livestock and poultry wastewater (LPWW) rich in ammonium nitrogen (NH-N) and antibiotics leads to eutrophication, and contributes to the risk of creating drug-resistant pathogens. The design-build-test-learn strategy was used to engineer a continuous process using Chlorella vulgaris to remove NH-N and antibiotics. The optimized system removed NH-N at a rate of 306 mg/L/d, degraded 99 % of lincomycin, and reduced the hydraulic retention time to 4 days.

Network meta-analysis of integrated traditional Chinese and Western medicine in the treatment of Sjogren's syndrome.

Objective: To evaluate efficacy and safety of traditional Chinese medicine (TCM) combined with Western medicine in treatment of Sjogren's syndrome (SS).

Methods: CNKI, WanFang, VIP, CBM, Sinomed, PubMed, Embase, and Web of Science were searched to collect randomized controlled trials (RCTs) of TCM combined with conventional western medicine (CWM) in treating SS from the time of their estalishment to May 2023. The researchers independently screened the literature and extracted data for quality evaluation.

Mesoporous Silica Nanoparticles Carrying Ligustrazine Inhibit Metastatic Properties of Colon Cancer Cells.

Objective: Finding methods that can interfere with Wnt/β-catenin signaling has become an important research direction in inhibiting colon cancer metastasis. Mesoporous silica nanoparticles can efficiently carry and release drugs. Therefore, combining ligustrazine, miR-570, and mesoporous silica nanoparticles as carriers will provide a theoretical basis for development of new therapeutic strategies and drugs.

Innovative plasma treatment of orange juice to improve bioactive concentration: The effects of various parameters using response surface analysis.

Orange juice is a highly nutritious beverage. Traditional pasteurization methods cause nutrient loss and taste changes. Plasma treatment (PT) is an emerging method with a high sterilization rate.

Rational design of diblock copolymer enables efficient cytosolic protein delivery.

Polymer-mediated cytosolic protein delivery demonstrates a promising strategy for the development of protein therapeutics. Here, we propose a new designed diblock copolymer which realizes efficient cytosolic protein delivery both in vitro and in vivo. The polymer contains one protein-binding block composed of phenylboronic acid (PBA) and N-(3-dimethylaminopropyl) (DMAP) pendant units for protein binding and endosomal escape, respectively, followed by the response to ATP enriched in the cytosol which triggers the protein release.

Development of an enzyme-linked immunosorbent assay using a monoclonal antibody to a dominant epitope in non-structural protein 4 of porcine reproductive and respiratory syndrome virus.

Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is one of the most severe swine diseases causing great economic losses for the international swine industry. Non-structural protein 4 (NSP4) is critical to the life cycle of PRRSV and contains dominant B cell epitopes. This study prepared a monoclonal antibody against Nsp4, and 2D11, which contained the sequence KQGGGIVTRPSGQFCN, was confirmed as the epitope.

An innovative exploration to identify and isolate the dominant-flocculated-species from polytitanium chloride synthesized by electrodialysis.

Abundance of dominant-flocculated-species is the key to determine coagulation performance of coagulant. Titanium-based coagulants have garnered considerable attention due to their high coagulation efficiency, but with a current challenge of the identification and isolation of the dominant-flocculated-species. Herein, polytitanium chloride (PTC), enriched with dominant-flocculated-species, was successfully synthesized by electrodialysis through accurate micro-interface control of the reaction among Ti-hydrolyzed-species and OH.

Spiral Large-Dimension Microfluidic Channel for Flow-Rate- and Particle-Size-Insensitive Focusing by the Stabilization and Acceleration of Secondary Flow.

Inertial microfluidics has demonstrated its ability to focus particles in a passive and straightforward manner. However, achieving flow-rate- and particle-size-insensitive focusing in large-dimension channels with a simple design remains challenging. In this study, we developed a spiral microfluidic with a large-dimension channel to achieve inertial focusing.

Co-fermentation of titanium-flocculated-sludge with food waste towards simultaneous water purification and resource recovery.

Recovery of resources from domestic sewage and food waste has always been an international-thorny problem. Titanium-based flocculation can achieve high-efficient destabilization, quick concentration and separation of organic matter from sewage to sludge. This study proposed co-fermentation of the titanium-flocculated sludge (Ti-loaded sludge) and food waste towards resource recovery by converting organic matter to value-added volatile fatty acids (VFAs) and inorganic matter to struvite and TiO nanoparticles.

Dynamic Transformation of Nano-MoS in a Soil-Plant System Empowers Its Multifunctionality on Soybean Growth.

Molybdenum disulfide (nano-MoS) nanomaterials have shown great potential for biomedical and catalytic applications due to their unique enzyme-mimicking properties. However, their potential agricultural applications have been largely unexplored. A key factor prior to the application of nano-MoS in agriculture is understanding its behavior in a complex soil-plant system, particularly in terms of its transformation.

Deep learning models for preoperative T-stage assessment in rectal cancer using MRI: exploring the impact of rectal filling.

Background: The objective of this study was twofold: firstly, to develop a convolutional neural network (CNN) for automatic segmentation of rectal cancer (RC) lesions, and secondly, to construct classification models to differentiate between different T-stages of RC. Additionally, it was attempted to investigate the potential benefits of rectal filling in improving the performance of deep learning (DL) models.

Methods: A retrospective study was conducted, including 317 consecutive patients with RC who underwent MRI scans.

Effectiveness of a 10-year continuous reduction of controlled-release nitrogen fertilizer on production, nitrogen loss and utilization of double-cropping rice.

Considerable literature has demonstrated the advantage of controlled-release nitrogen (CRN) fertilizer in improving crop productivity. However, few researches have explored the long-term impacts of using CRN fertilizers as alternative to common urea on production and N utilization in double-cropping paddy. To address this gap, our study utilized a database derived from a 10-year field experiment from 2013 to 2022.

Flow-Rate-Insensitive Plasma Extraction by the Stabilization and Acceleration of Secondary Flow in the Ultralow Aspect Ratio Spiral Channel.

Although microfluidic devices have made remarkable strides in blood cell separation, there is still a need for further development and improvement in this area. Herein, we present a novel ultralow aspect ratio (/ = 1:36) spiral channel microfluidic device with ordered micro-obstacles for sheathless and flow-rate-insensitive blood cell separation. By introducing ordered micro-obstacles into the spiral microchannels, reduced magnitude fluctuations in secondary flow across different loops can be obtained through geometric confinement.

Utilization of halophytes in saline agriculture and restoration of contaminated salinized soils from genes to ecosystem: Suaeda salsa as an example.

Halophytes can be used to screen genes for breeding salt-tolerant crops and are of great value in the restoration of salinized or contaminated soils. However, the potential of halophytes in improving saline soils remains limited. In this paper, based on the latest research progress, we use Suaeda salsa L.

High-Throughput Blood Plasma Extraction in a Dimension-Confined Double-Spiral Channel.

Microfluidic technologies enabling the control of secondary flow are essential for the successful separation of blood cells, a process that is beneficial for a wide range of medical research and clinical diagnostics. Herein, we introduce a dimension-confined microfluidic device featuring a double-spiral channel designed to regulate secondary flows, thereby enabling high-throughput isolation of blood for plasma extraction. By integrating a sequence of micro-obstacles within the double-spiral microchannels, the stable and enhanced Dean-like secondary flow across each loop can be generated.

[Clinical and genetic analysis of a child with X-linked dominant Alport syndrome].

Objective: To investigate the clinical features and genetic variant of a child with X-linked dominant Alport syndrome (XLAS).

Methods: A child who had presented at the First Affiliated Hospital of Zhengzhou University in May 2019 was selected as the study subject. Clinical data of the child was collected.

Molybdenum Nanofertilizer Boosts Biological Nitrogen Fixation and Yield of Soybean through Delaying Nodule Senescence and Nutrition Enhancement.

Soybean () is a crop of global significance and has low reliance on N fertilizers due to its biological nitrogen fixation (BNF) capacity, which harvests ambient N as a critical ecosystem service. BNF can be severely compromised by abiotic stresses. Enhancing BNF is increasingly important not only to alleviate global food insecurity but also to reduce the environmental impact of agriculture by decreasing chemical fertilizer inputs.

Satellite record reveals 1960s acceleration of Totten Ice Shelf in East Antarctica.

Wilkes Land and Totten Glacier (TG) in East Antarctica (EA) have been losing ice mass significantly since 1989. There is a lack of knowledge of long-term mass balance in the region which hinders the estimation of its contribution to global sea level rise. Here we show that this acceleration trend in TG has occurred since the 1960s.