Publications by authors named "Tia Utami"
Article Synopsis
- Nonalcoholic fatty liver disease (NAFLD) can lead to severe liver issues and the study investigated how palmitic acid (PA), a common dietary fat, affects liver cells using organ-on-a-chip technology.
- After exposure to palmitic acid, the liver cells showed a decrease in liver transcription factor activity and expression changes in 318 genes, indicating early signs of liver cell dedifferentiation.
- Despite these changes, there was no lipid accumulation in the cells, but an increase in collagen production was observed, suggesting that palmitic acid contributes to the early stages of lipotoxicity associated with NAFLD.
Recent advancements in bioengineering have introduced potential alternatives to liver transplantation via the development of self-assembled liver organoids, derived from human-induced pluripotent stem cells (hiPSCs). However, the limited maturity of the tissue makes it challenging to implement this technology on a large scale in clinical settings. In this study, we developed a highly efficient method for generating functional liver organoids from hiPSC-derived carboxypeptidase M liver progenitor cells (CPM+ LPCs), using a microwell structure, and enhanced maturation through direct oxygenation in oxygen-permeable culture plates.
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- Human pluripotent stem cell-derived liver organoids (HLOs) show promise for liver regenerative therapy, requiring large amounts of hiPSC-derived liver cells for transplantation.
- A new dialysis-based medium conditioning method was developed to enhance the hepatic differentiation of hiPSCs by efficiently accumulating essential growth factors.
- This approach allowed for high-density production of HLOs, achieving better or comparable liver cell functionality while minimizing growth factor usage and costs.
Oxygen, as an external environmental factor, plays a role in the early differentiation of human stem cells, such as induced pluripotent stem cells (hiPSCs). However, the effect of oxygen concentration on the early-stage differentiation of hiPSC is not fully understood, especially in 3D aggregate cultures. In this study, we cultivated the 3D aggregation of hiPSCs on oxygen-permeable microwells under different oxygen concentrations ranging from 2.
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