Publications by authors named "Tia Salter"
Article Synopsis
- There is an urgent need for new antibiotics due to increasing bacterial resistance to existing ones, with the bacterial Sec system being a promising target for this research.* -
- A new, scalable assay for screening Sec inhibitors using split NanoLuc luciferase has been developed, allowing for testing in living cells and distinguishing specific Sec targets.* -
- Initial tests on a library of 5000 compounds have identified some moderate inhibitors of Sec, showcasing the assay's effectiveness, with hopes for scaling up to find more potent options for drug development.*
Jasmonate-induced protein 60 (JIP60) is a ribosome-inactivating protein (RIP) from barley () and is involved in the plant immune response dependent on jasmonate hormones. Here, we demonstrate in that transient expression of the N-terminal domain of JIP60, from which the inhibitor domain (amino acids 163-185) is removed, initiates cell death, leading to extensive necrosis of leaf tissues. We used structure prediction of JIP60 to identify potential catalytic amino acids in the active site and tested these by mutagenesis and in planta assays of necrosis induction by expression in , as well as through an in vitro translation-inactivation assay.
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