PCR for the detection of Streptobacillus moniliformis.

Streptobacillus moniliformis is a Gram-negative bacterium found in various laboratory animal species and is the cause of rat bite fever and Haverhill fever in man. In order to evaluate a polymerase chain reaction (PCR) for the detection of this zoonotic bacterium in animal tissues a set of primers was designed based on the DNA base sequence of part of the 16S rRNA gene from 11 S. moniliformis strains.

Transmission of rat and guineapig Haemophilus spp. to mice and rats.

Mice and rats, free from Pasteurellaceae, were exposed to Haemophilus spp. (V-factor dependent Pasteurellaceae) by housing in proximity to infected rats or guinea pigs, and monitored by culture and enzyme-linked immunosorbent assay (ELISA) for cross infection. A minority of mice became infected when exposed to Haemophilus-infected rats but none when exposed to guinea pigs.

Comparison of assays for antibodies to Encephalitozoon cuniculi in rabbits.

Two indirect immunofluorescence (IIF) assays, two enzyme-linked immunosorbent assays (ELISAs) and the carbon immunoassay (CIA) for determination of antibodies to Encephalitozoon cuniculi were compared using 210 sera of rabbits, 135 of which originated from seven infected colonies, while 75 originated from four uninfected colonies. There was no evidence of a difference between the different assays with respect to the number of positive sera. There was a clear correlation between the quantitative response measured by IIF and CIA and the other assays, and between both IIF tests, while no such correlation was found in the quantitative response measured by ELISAs, which might be explained by the less quantitative nature of the ELISA.

Serological relationship of some V-factor dependent Pasteurellaceae (Haemophilus sp.) from guineapigs and rabbits.

Culture of guinea pig and rabbit respiratory tracts for bacteria using X- (haemin) and V- (NAD) factor in agar media detected infection by V-factor dependent Pasteurellaceae (Haemophilus sp.) in three colonies of guinea pigs and a group of rabbits. The 12 Haemophilus strains comprised three API NH codes classed as Haemophilus parainfluenzae and two codes classed as Haemophilus aphrophilus/paraphrophilus.

Growth medium affects the cellular fatty acid composition of Pasteurellaceae.

We studied the cellular fatty acid composition of 10 Actinobacillus (A.) and Pasteurella (P.) reference strains grown on 2 types of agar by the MIDI Microbial Identification System (MIS).

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies.

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity.

Serological studies of Corynebacterium kutscheri and coryneform bacteria using an enzyme-linked immunosorbent assay (ELISA).

An enzyme-linked immunosorbent assay (ELISA) to measure Corynebacterium kutscheri antibodies in mice and rats was developed. Seven C. kutscheri isolates showed considerable serological relationship, but Japanese isolates differed from the British isolates.

An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies to SP group Pasteurellaceae in guineapigs.

Cross-reactivity studies with Pasteurellaceae from guineapigs revealed 5 serologically distinct groups, comprising Pasteurella multocida, Sp group bacteria, SP-like bacteria, Pasteurella pneumotropica and an actinobacillus-like bacterium. Guineapig Pasteurellaceae differed serologically from mouse-derived P. pneumotropica NCTC 8284.

Multifactorial analysis of antibiotic sensitivity of Bordetella bronchiseptica isolates from guineapigs, rabbits and rats.

The possible relationship between antibiotic sensitivity of B. bronchiseptica isolates and host species was examined. Growth inhibition of 11 B.

Assessment of occupational exposure to diesel exhaust. The use of an immunoassay for the determination of urinary metabolites of nitroarenes and polycyclic aromatic hydrocarbons.

In a repair shop for train engines a pilot study was conducted to investigate occupational exposure to diesel exhaust. 1-Nitropyrene was determined in stationary sampled total suspended particulate matter collected on 2 consecutive workdays. Air concentrations of particulate associated 1-nitropyrene varied from non-detectable to 5.

Colonization and antibody response in mice and rats experimentally infected with Pasteurellaceae from different rodent species.

Mice and rats were experimentally infected with Pasteurellaceae isolated from mice, rats, hamsters and gerbils. Mice and rats were most heavily colonized by strains originally isolated from mice and rats respectively, and to a lesser extent by Pasteurellaceae from hamsters and gerbils. Colonization was generally accompanied by seroconversion.

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Streptobacillus moniliformis antibodies.

An enzyme-linked immunosorbent assay (ELISA) to measure Streptobacillus moniliformis antibodies in mice and rats was developed. Twelve S. moniliformis strains originating from cases of rat-bite fever and Haverhill fever in man and from various rodent species, showed considerable serological relationship.

An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies.

An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs.

Hemagglutination by Pasteurellaceae isolated from rodents.

Pasteurellaceae notably P. pneumotropica, have been associated with severe outbreaks of respiratory disease in several species of rodents. Host-specific parasitism of Pasteurellaceae in rodents has hardly been studied.