A sensitive method for the determination of per- and polyfluoroalkyl substances in food and food contact material using high-performance liquid chromatography coupled with tandem mass spectrometry.

Dietary intake is the major pathway of human exposure to per- and polyfluoroalkyl substances (PFAS). Due to their generally very low concentrations in food, especially for foods of plant origin, and their toxicological relevance, demand is growing for improved selective and sensitive analytical methods for the determination of PFAS in the lower ng/kg range. The relevance is pointed out due to the fact that the European Commission has published limits of quantification (LOQs) in the lower ng/kg range for different food matrices in Recommendation (EU) 2022/1431 on the monitoring of perfluoroalkyl substances in food.

Transfer of cannabinoids into the milk of dairy cows fed with industrial hemp could lead to Δ-THC exposure that exceeds acute reference dose.

The industrial hemp sector is growing and, in recent years, has launched many novel hemp-derived products, including animal feed. It is, however, unclear to what extent individual cannabinoids from industrial hemp transfer from the feed into products of animal origin and whether they pose a risk for the consumer. Here we present the results of a feeding experiment with industrial hemp silage in dairy cows.

Unraveling Congener-Specific Transfer of Non-Dioxin-Like Polychlorinated Biphenyls (ndl-PCBs) from Feed into Chicken Meat.

Non-dioxin-like polychlorinated biphenyls (ndl-PCBs) are a subclass of persistent bioaccumulative pollutants able to enter the food chain. We investigated the transfer of ndl-PCBs from contaminated feed into meat and liver of fattening chickens. A total of 48 chicks were divided into five treatment and one control groups.

Structural Identification and Quantification of Chlorinated Paraffins in Fish Samples Using Comprehensive Two-Dimensional Gas Chromatography with Negative Chemical Ionization Quadrupole Time-of-Flight Mass Spectrometry and Comparison to a Direct Injection-Atmospheric Pressure Chemical Ionization-Orbitrap/Mass Spectrometry Method.

This study presents a comprehensive two-dimensional gas chromatography with negative chemical ionization quadrupole time-of-flight mass spectrometry (GC × GC-NCI-QTOF/MS) method, which allows for a precise chromatographic separation of short-chain chlorinated paraffins (SCCPs) and medium-chain chlorinated paraffins (MCCPs). A new reversed-phase column setup was used, which allows for more accurate separation of MCCPs compared to known GC × GC methods. In a pilot study, 25 freshwater fish samples were analyzed with this method to characterize chlorinated paraffin (CP) compositions.

Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs.

Recent European regulations have indicated the need for new bioanalytical screening methods capable of monitoring dioxin and dioxin-like compounds in foodstuffs and environmental samples, cost-effectively and with a quicker turnaround. Cryo-cells of the hepatic H4IIE line preserved in 96-well plates were exposed to sample extracts prepared from various foodstuffs and analysed for their content of dioxins and dioxin-like compounds by means of the 7-Ethoxyresorufin-O-Deethylase (EROD)-assay in two laboratories. Assay data were compared between both laboratories and results from instrumental analysis used as a confirmatory method.

Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiation.

UVB radiation-induced signaling in mammalian cells involves two major pathways: one that is initiated through the generation of DNA photoproducts in the nucleus and a second one that occurs independently of DNA damage and is characterized by cell surface receptor activation. The chromophore for the latter one has been unknown. Here, we report that the UVB response involves tryptophan as a chromophore.