Implementation of a structured care programme for diabetes mellitus in a defined population in mid-Sweden.

A local programme for diabetes care, based on the Swedish national programme, was introduced in the Köping-Arboga-Kungsör area in mid-Sweden in 1984. Before the programme was implemented, a survey of diabetes care was performed in the area. Two years later, a new survey involving 92% of 1253 diabetes patients in the area was performed to see if the programme was associated with an improved level of care, more optimal treatment and better metabolic control.

Intervention against ischaemic heart disease risk factors in primary health care in a semi-rural community. The population study "50-year-old people in Kungsör".

Objective: To see whether a program for screening and intervention against ischaemic heart disease (IHD) risk factors could be integrated into the ordinary work of a primary health care centre.

Design: Longitudinal population study with baseline screening, intervention, and one-year follow up.

Setting: Kungsör, a semi-rural community in mid-Sweden.

Myocardial infarction risk factors and well-being among 50-year-old women before and after the menopause. The population study "50-year-old people in Kungsör".

Objective: To examine whether early menopause has a negative influence on the traditional ischaemic heart disease (IHD) risk factor pattern and on well-being.

Design: Cross-sectional population study.

Setting: Kungsör, a semirural community in mid-Sweden.

Myocardial infarction risk factors in a semirural area in mid-Sweden. The population study "50-year-old people in Kungsör".

The mortality from ischaemic heart disease is higher in predominantly rural northern and western Sweden than in the more urban eastern and southern districts. This study was performed in a small semirural area in mid-Sweden with lower mortality from ischaemic heart disease in middle-aged men and higher mortality in middle-aged women than the national average. Smoking habits, serum cholesterol, and blood pressure were measured in all 50-year-old men and women in the community (n = 314) during a four-year period.

Well-being among 50-year-old people. The population study "50-year-old people in Kungsör".

Self-assessed well-being has been shown to be related to earlier medical events and to be a predictor of mortality. 50-year-old men and women (n = 314) in Kungsör were invited to an examination for traditional risk factors for cardiovascular diseases and different aspects of self-assessed well-being. The differences between men and women concerning their well-being could not explain the differences in the expected mortality between the sexes.

Pulsatile administration of gonadotropin releasing hormone (Gn-RH) to lactating sows using a portable automatic pump ("Zyklomat").

The object of this investigation was to study the feasibility of using a “Zyklomat roller peristaltic pump” for pulsatile administration of gonadotropin releasing hormone (Gn-RH) in primiparous lactating sows. Four primiparous sows were used. The pump catheter was inserted into a jugular vein on day 8 or day 22 of lactation.

Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levels.

A cytofluorimeter is described, using the combination of Argon UV (351-363 nm) and Argon Blue (488 nm) lasers. The dual excitation makes it possible to monitor simultaneously the redox state of flavins and NAD(P)H as indicative of cell metabolic state. Light scatter, absorption, and staining with exogenous fluorescent dyes can add additional information.

Metabolic control and compartmentation in single living cells.

Microspectrofluorometry of cell coenzymes (NAD(P)H, flavins) in conjunction with sequential microinjections into the same cell of metabolites and modifiers, reveals aspects of the regulatory mechanisms of transient redox changes of mitochondrial and extramitochondrial nicotinamide adenine dinucleotides. The injection of ADP in the course of an NAD(P)H transient produced by glycolytic (e.g.

The differential effects of the carcinogen dimethylnitrosamine on isocitrate and 6-phosphogluconate metabolism in single intact cells.

A microspectrofluorimetric study is made of the influence of dimethylnitrosamine on NADP reduction, following sequential microinjections into the same L cell, of two substrates: (1) isocitrate, with activity of isocitrate dehydrogenase both in the extramitochondrial and intramitochondrial compartments, (2) 6-phosphogluconate, with activity of the dehydrogenase in the extramitochondrial compartment. In control L cells a two-step reduction of NAD(P) is obtained followed by relatively slow reoxidation. In the minutes which follow addition of carcinogen, e.

Design and synthesis of new reactive fluorescent dyes for cytofluorometry.

Our purpose has been to synthesize reactive fluorescent compounds with different excitation and/or emission spectra that can be used for multiple fluorescence analysis in combination with the "natural" cell fluorescence exhibited by reduced coenzyme (NAD(P)H) or flavins. Synthesis of condensation products of p-bis(2-chloroethyl)-amino-benzaldehyde is described. Their absorption spectra range from 360 to 480 nm and their emission spectra lie between 520 and 590 mm.

Flow cytometric analysis of cellular endogenous fluorescence simultaneously with emission from exogenous fluorochromes, light scatter and absorption.

A cytofluorimeter capable of simultaneous 4-parameter analysis (i.e., endogenous fluorescence (NAD(P)H), exogenous fluorescent dyes, absorption and small angle scatter) is described.

Multichannel analysis of intracellular control and intercellular transfer of molecules.

The metabolic regulation and exchanges within intracellular organelles or a cell cluster are studied by multichannel microfluorometry and microinjection of metabolites or tracers. The determination of structure-function relationships relies on the retrieval of cells after microfluorometry, for subsequent morphological evaluation. Rate constants of coenzyme reduction-reoxidation were deduced from a mathematical model of NAD(P) in equilibrium with NAD(P)H transients due to microinjection of metabolites into cultured cells belonging to a variety of normal or malignant lines.

Intracellular red-ox steady states as basis for cell characterization by flow cytofluorometry.

The blue fluorescence of intracellular reduced coenzymes [NAD(P)H] can be used for cell characterization by rapid flow cytofluorometry. Different types of cells show different metabolic (red-ox) steady states under given conditions. Examples are given for yeast and isolated rat liver cells.

Quantitative reflection contrast microscopy of living cells.

Mammalian cells in culture (BHK-21, PtK2, Friend, human flia, and glioma cells) have been observed by reflection contrast microscopy. Images of cells photographed at two different wavelengths (546 and 436 nm) or at two different angles of incidence allowed discrimination between reflected light and light that was both reflected and modulated by interference. Interference is involved when a change in reflected intensity (relative to glass/medium background reflected intensity) occurs on changing either the illumination wavelength or the reflection incidence angle.

Intercellular communication in pancreatic islet monolayer cultures: a microfluorometric study.

Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry. Fluorescein spread from an injected islet cell directly into neighboring islet cells, and, in the presence of 16.7 millimolar glucose, significantly more islet cells communicated with the injected cell than in glucose-free medium.

Metabolic rates and intercellular transfer of molecules in cultures of human glia and glioma cells.

Metabolic rates and intercellular transfer of metabolites were studied in human glia and glioma culture cells via topographic scan of NAD(P)H fluorescence by multichannel microfluorometry in conjunction with microinjection of glucose-6-P + allosteric activators. Metabolic rates evaluated from NAD(P) in equilibrium NAD(P)H transients and the required substrate levels were 3--4 times lower in glioma cells as compared to glia cells. Both glia and glioma cells showed variability in the occurrence of intercellular metabolite transfer, detectable via observation of a transient in a neighbour of the cell injected with substrate.

The effect of atebrine and an acridine analog (BCMA) on the coenzyme fluorescence spectra of cultured melanoma and Ehrlich ascites (EL2) cells.

Coenzyme fluorescence spectra of single living cells are due to free pyridine nucleotides (folded configuration), bound pyridine nucleotides (unfolded configuration) and a third component, possibly a mixture or flavins. Such spectra can be used to recognize possible differences in coenzyme composition between cell lines or changes of metabolic pathways due to chemicals acting at levels below or above cytotoxicity, by high resolution spectrofluorometry. A study of spectra recorded from cultured Ehrlich ascites (EL2), and Harding Passey melanoma cells (HPM-67 and HPM-73 line) grown under comparable conditions, shows that free NAD(P)H predominates in HPM-67 and EL2, while this coenzyme is bound in HPM-73.

Characterization of the normoblast population in beta-thalassaemic blood by rapid-flow cytofluorometry.

Samples of peripheral blood from patients with beta-thalassaemia major which contained significant numbers of nucleated normoblasts were stained with acridine orange and analyzed with rapid-flow cytofluorometry. The pyknotic normoblast-nuclei gave less green 'DNA' fluorescence than the (diploid) leucocytes and constituted a separate, distinct subpopulation. Mean values of the fluorescence intensities and standard deviations as displayed by multichannel analyses gave a numerical value for normoblasts with regard to their maturation stages.

Mithramycin fluorescence for quantitative determination of deoxyribonucleic acid in single cells.

The use of the antibiotic drug mithramycin for cytoflurometric assessment of deoxyribonucleic acid in single cells has been studied in smears of a standard cell population of rat thymocytes. The optimal staining conditions have been determined including the influence of fixation (freeze-drying, formalin and ethanol). The drug equilibration time has been estimated in relation to the concentration of the mithramycin and to Mg++ was found to enhance the fluorescence intensity produced by the mithramycin-deoxyribonucleic acid interaction.