Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone.

Thermodisinfection of human femoral heads from living donors harvested during hip joint replacement is an established processing procedure. This study was designed to examine the influence of heat sterilization on pull out strength of cancellous bone and storage at different temperatures up to 2 years since we had previously studied the storage of unprocessed cancellous bone. Porcine cancellous bone resembling human bone structure was obtained from 140 proximal humerus of 6-8 months old piglets.

Effect of Storage Temperature on Allograft Bone.

BACKGROUND: The recommendations for storage temperature of allogeneic bone are varying between -20 °C and -70 °C and down to -80 °C. The necessary temperature of storage is not exactly defined by scientific data, and the effect of different storage temperatures onto the biomechanical and the biological behavior is discussed controversially. METHODS: The historical development of storage temperature of bone banks is described.

The sternal fracture: radiographic analysis of 200 fractures with special reference to concomitant injuries.

Background: The assessment of the sternal fracture and concomitant injuries is discussed.

Methods: Two hundred sternal fractures were analyzed with respect to accident circumstances, fracture morphology and topography, and associated injuries.

Results: Traffic accidents were frequent; 89.

Validation of the 'Marburg bone bank system' for thermodisinfection of allogenic femoral head transplants using selected bacteria, fungi, and spores.

The Marburg Bone Bank System 'Lobator sd-2' is widely used to process human femoral heads removed during aseptic surgery by thermal disinfection. The inactivating capacity of the thermodisinfection system was validated in compliance with current standards using a newly developed femoral head model. The following micro-organisms, bacteria and fungi, taken from the American Type Culture Collection were investigated: Staphylococcus aureus, Staphyloccus epidermidis, Enterococcus faecium, Pseudomonas aeruginosa, Bacillus subtilis including spores, Clostridium sporogenes, Mycobacterium terrae, Candida albicans and Aspergillus niger spores.

Lavage of contaminated surfaces: an in vitro evaluation of the effectiveness of different systems.

Introduction: Lavage is an effective, additive therapeutic procedure with a broad application in surgery. In addition to irrigation with the conventional syringe, the pulsed jet lavage system is used. The effectiveness of bacterial reduction depends on, among other things, the irrigation pressure.

Virus inactivation in bone tissue transplants (femoral heads) by moist heat with the 'Marburg bone bank system'.

Several virus inactivation procedures like heat treatment, gamma irradiation and chemical sterilization are used to increase the safety of bone tissue transplants. In this study we present data on the virus-inactivating effect of heat disinfection on human femoral heads, using the Marburg bone bank system 'Lobator sd-2'. Three enveloped viruses (human immunodeficiency virus type 2 [HIV-2], bovine viral diarrhoea virus as a model for Hepatitis C virus [HCV], and the herpesvirus pseudorabies virus), and three non-enveloped viruses (hepatitis A virus, poliomyelitis virus, and bovine parvovirus) were investigated.