Characterization of eggplant grown in animal manure amended soil.

A field experiment was conducted with native soil; sewage sludge (SS); horse manure (HM); chicken manure (CM); vermicompost, organic fertilizer and inorganic fertilizer mixed with the native soil. The soil in each of the seven treatments was also mixed with biochar and planted with eggplant. Eggplant fruits were analyzed for vitamin C, total phenols, and soluble sugars.

Emerging technology for increasing glucosinolates in arugula and mustard greens.

Two plant species, arugula (Eruca sativa) and mustard (Brassica juncea) were field-grown under four soil management practices: soil mixed with municipal sewage sludge (SS), soil mixed with horse manure (HM), soil mixed with chicken manure (CM), and no-mulch bare soil (NM) to investigate the impact of soil amendments on the concentration of glucosinolates (GSLs) in their shoots. GSLs, hydrophilic plant secondary metabolites in arugula and mustard were extracted using boiling methanol and separated by adsorption on sephadex ion exchange disposable pipette tips filled with DEAE, a weak base, with a net positive charge that exchange anions such as GSLs. Quantification of GSLs was based on inactivation of arugula and mustard myrosinase and liberation of the glucose moiety from the GSLs molecule by addition of standardized myrosinase (thioglucosidase) and spectrophotometric quantification of the liberated glucose moiety.