The medulloblastoma cell line DAOY but not eleven other tumor cell lines expresses minichromosome maintenance protein 4.

Minichromosome maintenance proteins (MCM) are required for initiation and elongation of chromosomal DNA, ensuring that DNA replication takes place only once. Although MCMs are considered of utmost importance in tumor biology and as potential marker proteins, they were not unambiguously identified at the protein level and we therefore aimed to characterize MCM 4 in a medulloblastoma cell line and provide a protein chemical analytical tool. In addition, we searched for this protein in twelve tumor cell lines and a series of non-tumor cells.