Publications by authors named "Thomas Skelly"
Article Synopsis
- Gene isoforms perform different functions in response to environmental changes, making their study crucial for understanding biological processes and diseases, but identifying them across genomes is technically challenging due to their high sequence similarity.
- Traditional methods like Sanger sequencing are time-consuming and inefficient, while next-generation sequencing (NGS) technologies face issues with short read lengths that complicate accurate gene assembly.
- The new analysis pipeline integrating long-read data from the PacBio platform successfully profiles similar gene isoforms and has shown superior performance in reconstructing known isoforms compared to traditional NGS techniques.
Towards Better Precision Medicine: PacBio Single-Molecule Long Reads Resolve the Interpretation of HIV Drug Resistant Mutation Profiles at Explicit Quasispecies (Haplotype) Level.
J Data Mining Genomics Proteomics
January 2016
Development of HIV-1 drug resistance mutations (HDRMs) is one of the major reasons for the clinical failure of antiretroviral therapy. Treatment success rates can be improved by applying personalized anti-HIV regimens based on a patient's HDRM profile. However, the sensitivity and specificity of the HDRM profile is limited by the methods used for detection.
View Article and Find Full Text PDFBackground: A minor but significant fraction of samples subjected to next-generation sequencing methods are either mixed-up or cross-contaminated. These events can lead to false or inconclusive results. We have therefore developed SASI-Seq; a process whereby a set of uniquely barcoded DNA fragments are added to samples destined for sequencing.
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