Publications by authors named "Thomas Retzer"
Isolation and Analysis of Rare Enzymatic Events with Multiplex Flow Magnetic Tweezers.
Methods Mol Biol
December 2024
Article Synopsis
- New techniques in biomolecular dynamics allow manipulation of forces at the single-molecule level, helping to advance our understanding.
- A newly developed method significantly increases the throughput of force spectroscopy, enabling analysis of thousands of molecules simultaneously, including rare enzymatic events.
- The chapter includes experimental procedures for studying DNA gyrase's supercoiling dynamics and introduces a software platform to classify dynamic behaviors, making it applicable to various complex enzymatic processes.
The rapid development of new imaging approaches is generating larger and more complex datasets, revealing the time evolution of individual cells and biomolecules. Single-molecule techniques, in particular, provide access to rare intermediates in complex, multistage molecular pathways. However, few standards exist for processing these information-rich datasets, posing challenges for wider dissemination.
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