Diagnostic Ambiguity Caused by an Atypical e18a2 Transcript in a Chronic Myeloid Leukemia Patient.

We describe the case of a chronic myeloid leukemia (CML) patient with a rare atypical e18a2 :: transcript. The generation of this transcript was explained by a detailed molecular analysis, including the identification of both chromosomal breakpoints (:: on der(22) and :: on der(9)) at the genomic level. The use of a cryptic splice site in intron 1 of led to the generation of an in-frame :: fusion transcript.

p14(ARF)-induced apoptosis in p53 protein-deficient cells is mediated by BH3-only protein-independent derepression of Bak protein through down-regulation of Mcl-1 and Bcl-xL proteins.

The p14(ARF) tumor suppressor plays a central role in regulating cell cycle arrest and apoptosis. We reported previously that p14(ARF) is capable of triggering apoptosis in a p53-independent manner. However, the mechanism remained unclear.